Rapid and sensitive analysis of three polyphenols in tobacco by CE using homemade C⁴D with a mini detection cell

Abstract

A rapid, sensitive, and practical CE with C(4) D detection was developed for the analysis of three polyphenols (rutin, scopoletin, and chlorogenic acid) in tobacco samples. The constructed mini detection cell (12 mm × 10 mm × 10 mm) of C(4) D featured with small inner cell volume (∼2 nL), smaller noise (<0.9 mV), repeatability, high strength and durableness. Three polyphenols were ultrasonically extracted with methanol-water (70:30, v/v) solution following SPE cleanup. The CE method was optimized with the running buffer of 150 mmol L(-1) 2-amino-2-methyl-1-propanol (pH 11.2), and the applied separation voltage of +20 kV over a capillary of 50 μm id × 375 μm od × 50 cm (38 cm to the C(4) D window, 41.5 cm to the UV detector window), which gave a baseline separation of three polyphenols within ca. 6 min. The method provided the limits of quantification (S/N = 10) at about 0.08-0.15 μg g(-1) for three polyphenols, whereas the overall recoveries ranged from 82% to 88%. The proposed method has been successfully applied to measure three polyphenols in the actual tobacco samples, and their contents were calculated and evaluated.

Determination of oxalate and citrate in urine by capillary electrophoresis using solid‐phase extraction and capacitively coupled contactless conductivity based on an improved mini‐cell

“…For K + and Na + , the theoretical plates are about 15 800 and 17 900 plates/m, respectively. This is higher than the published figures , in which K + and Na + were reported to possess 8000 and 13 000 plates/m, respectively. Using the improved C 4 D mini‐cell, the noise was in the range of 0.2∼0.5 mV and changed with different CE buffer solutions.…”

“…The reproducibility and stability of the improved minicell used in C 4 D can reach the same level as reported previously [37] and in addition, the smaller inner cell volume contributes to an improvement in the column efficiency. For K + and Na + , the theoretical plates are about 15 800 and 17 900 plates/m, respectively.…”

“…Using the improved C 4 D mini‐cell, the noise was in the range of 0.2∼0.5 mV and changed with different CE buffer solutions. This noise level was smaller than 0.9 mV published previously .…”

Contactless conductivity detection for analytical techniques—Developments from 2012 to 2014

“…Other applications include the determination of three polyphenols in tobacco leafs , a set of 14 lanthanides in simulated spent nuclear reactor fuel , peroxycarboxylates in commercial peracetic acid , and PCR products in genetically modified soybeans . Enzymatic assays of myrosinase were evaluated on the basis of sulphate production and its subsequent CE‐C 4 D determination .…”

Influence of pH on the Effectiveness of Hydrogen Peroxide Whitening

“…One of the main polyphenol components of tobacco is rutin, 29-31 whose structure is shown in Figure 5B. 31 According to the measured values of DE, it is evident that the chromogen solution present in tobacco is more resistant to oxidation by hydrogen peroxide than the one present in wine. Such a difference may be due to the chemical structure of these molecules.…”