Rapid and selective recognition of <i>Vibrio parahaemolyticus</i> assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film

Fu, Kaiyue; Zhang, Huiwen; Guo, Yuanyuan; Li, Juan; Nie, Heran; Song, Xiaohong; Xu, Kun; Wang, Juan; Zhao, Chao

doi:10.1039/d0ra00306a

Cited by 14 publications

(6 citation statements)

References 39 publications

(37 reference statements)

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“…The method of Fu establishment, per fluorinated alkoxysilane modified molecularly imprinted polymer film, takes only two hours, but the detection line can only reach 10 4 cfu mL −1 . 40 It is obvious to see that in this assay the detection method was with higher sensitivity and more time-saving. This is attributed mainly to the effective immunomagnetic separation, high catalytic activity of IMS and sensitive QDs fluorescent reporting system.…”

Section: Resultsmentioning

confidence: 91%

Rapid detection of Vibrio parahaemolyticus using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence

Zhai

Meng

et al. 2021

RSC Adv.

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Section: Resultsmentioning

confidence: 91%

Rapid detection of Vibrio parahaemolyticus using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence

Zhai

Meng

et al. 2021

RSC Adv.

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“…However, after incubation of S. aureus (1 × 10 6 cfu mL –1 ), the fluorescence intensity dramatically increased for 15 min incubation and then remained stable. Finally, the incubation time of POTS-modified BIPF with S. aureus was 2 h according to our previous work . On the basis of these results, the optimum conditions were chosen here: (a) CA-CuNCs concentration, 4.64 mM; (b) DA-AuNPs concentration, 6.35 mM; (c) incubation time, 2 h; (d) fluorescence quenching time, 1 min; (e) fluorescence recovery time, 15 min.…”

Section: Results and Discussionmentioning

confidence: 99%

Label-Free Detection of Staphylococcus aureus Based on Bacteria-Imprinted Polymer and Turn-on Fluorescence Probes

Guo

Song

et al. 2020

ACS Appl. Bio Mater.

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The effective identification and quantitative determination of Staphylococcus aureus is a major public health concern. Here, an innovative strategy that combines a bacteria-imprinted polydimethylsiloxane film for bacterial recognition and fluorescence resonance energy transfer platform for turn-on fluorescence sensing is demonstrated. The bacteria-imprinted polydimethylsiloxane film was facilely fabricated to generate corresponding specific sites on the polydimethylsiloxane surface via stamp imprinting using Staphylococcus aureus as template followed by modification with 1H,1H,2H,2H-perfluorooctyltriethoxysilane. The fluorescence resonance energy transfer platform was developed through electrostatic interaction between citrate-functional copper clusters and dopamine-stabilized gold nanoparticles. When the Staphylococcus aureus are present, the 1H,1H,2H,2H-perfluorooctyltriethoxysilane-modified bacteria-imprinted polydimethylsiloxane film can precisely capture the target; subsequently, the negatively charged bacteria compete with citrate-functional copper clusters and bind to dopamine-stabilized gold nanoparticles, leading to the fluorescence recovery of citrate-functional copper clusters. The entire detection process was achieved within 135 min, showing a wide linear calibration response from 10 to 1 × 107 cfu mL–1 with a low detection limit of 11.12 cfu mL–1. Furthermore, the recoveries from spiked samples were from 97.7 to 101.90% with relative standard derivations lower than 10%. The established label-free assay of measuring Staphylococcus aureus is rapid, sensitive, specific, and efficient.

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“…Microcontact/stamp imprinting Microcontact imprinting has been extensively used to prepare MIPs that can detect different types of pathogenic bacteria [16,[40][41][42]. The processing procedure is straightforward:…”

Section: Whole Cell Imprintingmentioning

confidence: 99%

“…In contrast to natural receptors such as antibodies or enzymes, MIPs are highly physically and chemically stable and can be reused for a long period. Therefore, MIP-based sensors have been employed for the detection of a large variety of chemical and biological hazards in food products, ranging from small molecules (mycotoxins [10,11] and illegal food additives [12,13]) to macromolecules (allergenic proteins and bacterial Published in the topical collection Analytical Chemistry for Infectious Disease Detection and Prevention with guest editors Chaoyong Yang and XiuJun (James) Li. exotoxins) [14,15] and even larger analytes (pathogenic bacteria and viruses) [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Molecular imprinting technology for sensing foodborne pathogenic bacteria

Zhang

Wang

2021

Anal Bioanal Chem

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Foodborne diseases caused by bacterial pathogens pose a widespread and growing threat to public health in the world. Rapid detection of pathogenic bacteria is of great importance to prevent foodborne diseases and ensure food safety. However, traditional detection methods are time-consuming, labour intensive and expensive. In recent years, many attempts have been made to develop alternative methods for bacterial detection. Biosensors integrated with molecular imprinted polymers (MIPs) and various transducer platforms are among the most promising candidates for the detection of pathogenic bacteria in a highly sensitive, selective and ultra-rapid manner. In this review, we summarize the most recent advances in molecular imprinting for bacterial detection, introduce the underlying recognition mechanisms and highlight the applications of MIP-based biosensors. In addition, the challenges and future perspectives are discussed with the aim of accelerating the development of MIP-based biosensors and extending their applications.

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Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film

Abstract: Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts.

Cited by 14 publications

References 39 publications

Rapid detection of Vibrio parahaemolyticus using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence

Rapid detection of Vibrio parahaemolyticus using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence

Label-Free Detection of Staphylococcus aureus Based on Bacteria-Imprinted Polymer and Turn-on Fluorescence Probes

Molecular imprinting technology for sensing foodborne pathogenic bacteria

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