Rapid and selective determination of urinary lysozyme based on magnetic molecularly imprinted polymers extraction followed by chemiluminescence detection

Emprego de polímeros de impressão molecular em preparo de amostras para análise de compostos orgânicos: aplicações e tendências

“…O método demonstrou ser confiável e muito prático, deixando claro para os pesquisadores que este poderá ser muito promissor para análises de rotina a fim de se diagnosticar doenças renais [111] . Para análise simultânea de três esteróis (ergosterol, stigmasterol e β-sitosterol) em amostras complexas, um MIP magnético, utilizando β-sistrol como MM, foi sintetizado por Zhan et al [112] .…”

Magnetic-graphene based molecularly imprinted polymer nanocomposite for the recognition of bovine hemoglobin

“…The obtained magnetic MIPs can not only selectively recognition and separation the target molecule from mixture through imprinted polymer shell but also can be easily extracted from the reaction medium by an external magnet. For example, Jing et al [26,27] developed a chemiluminescence method employing magnetic molecularly imprinted polymers for the determination of lysozyme in human serum samples. Gao et al [28] exploited thin MIP to imprint Lyz, BHb, BSA and RNase based on magnetic Fe 3 O 4 nanoparticles (NPs) with a uniform core-shell structure for the recognition of proteins.…”

Heat induced aggregation of gold nanorods for rapid visual detection of lysozyme

“…Being a low molecular weight protein, it can be filtered easily through glomerulus and reabsorbed by the proximal tubular cells via the megalin receptor [15][16][17]. Excretion of elevated levels of lysozyme in urine could be a sign of damage to tubular cells of kidneys and myelomonocytic leukemia [15,18,19]. In certain cancers (especially myelomonocytic leukemia), there is excessive production of lysozyme by cancer cells that elevates to toxic levels in the blood.…”

“…In many cases, determination of lysozyme plays an important role in toxicological experiments and monitoring the influence of environmental pollution on human health. It can be used as a biomarker in occupational and environmental medicine (cadmium or mercury exposure) [15]. Therefore, development of a simple technique for monitoring this protein could be very important in diagnostic approaches.…”

“…With well-known structure, function, dynamics and physico-chemical properties [11][12][13][14], it is an important defense molecule of the innate immune system, mediating protection against microbial invasion. Being a low molecular weight protein, it can be filtered easily through glomerulus and reabsorbed by the proximal tubular cells via the megalin receptor [15][16][17]. Excretion of elevated levels of lysozyme in urine could be a sign of damage to tubular cells of kidneys and myelomonocytic leukemia [15,18,19].…”