Rapid and selective characterization of influenza virus constituents in monovalent and multivalent preparations using non-porous reversed-phase high performance liquid chromatography columns

Regulation of Antinucleoprotein IgG by Systemic Vaccination and Its Effect on Influenza Virus Clearance

“…In fact, transfer of NP-immune C57BL/6 (but not AID.S) mouse serum (3) or NP-specific MAb (25a) accelerates viral clearance in previously naive recipient mice. Seasonal trivalent inactivated influenza virus vaccine (TIV) is known to contain NP (4,18,19). It is also known that the amount of NP can vary somewhat among TIV preparations (4), and it is unclear how much of this antigen is available to B cells in a typical vaccine dose.…”

“…2) (4,18,19), we asked whether anti-NP antibody is induced by vaccination. Two human sample sets were analyzed: plasma from before and after 2008/09 TIV immunization (study 1) and serum from different individuals before and after 2003/04 TIV immunization (study 2).…”

“…The difficulty of performing cause-and-effect experiments in humans leaves it uncertain as to whether one or both can mediate cross-protection. Doubt about anti-NP antibody in particular has been raised, given the presence of NP in seasonal inactivated influenza virus vaccines (4,18,19), which are considered to be poorly cross-protective (2). This doubt is based upon the assumption that the NP in the vaccines induces high titers of specific antibody that is ineffective against virus encounter.…”

Influenza A Virus Matrix Protein 1-Specific Human CD8+T-Cell Response Induced in Trivalent Inactivated Vaccine Recipients

“…TIV induced a significant increase in influenza A virus-reactive T cells in 6-monthto 4-year-old children (LAIV was not evaluated in this age group) (11). We reported earlier that an influenza virus subunit vaccine which was presented with Iscomatrix significantly (20), and recently the presence of matrix protein 1 (M1) in TIV was reported by two groups (6,7,22). García-Cañas et al identified it by two-dimensional high-performance liquid chromatography and mass spectrometry in one of three TIVs analyzed (6), and Rastogi et al detected it by Western blotting using anti-M1 antibody (data were not shown in the article) (22).…”

Evaluation of pretransplant influenza vaccination in hematopoietic SCT: a randomized prospective study

“…This might be explained by that the ELISA measures IgG against epitopes from the hemagglutinin, the neuraminidase and trace amounts of the matrix 1 protein. [28][29][30][31] Apart from the neutralization epitopes of HA, these other epitopes may be beneficial in Ab-dependent cell-mediated cytotoxicity and complement fixation.…”