Rapid and reliable detection of HLA-specific antibodies with the luminex platform

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

“…Additionally, similar studies have been carried out using samples derived from various animal models (Keyes et al, 2003(Keyes et al, , 2002Hutchinson et al, 2001). The multiplex analysis capability of the Luminex technology is being adapted to other assays including antibody screening (Biagini et al, 2004(Biagini et al, , 2003Jones et al, 2002;Opalka et al, 2003;Pickering et al, 2002a,b;Martins, 2002), HLA typing Osowski et al, 2003;Chesterton et al, 2003;Brailey and Susskind, 2003), single nucleotide polymorphism or mutation analysis (Hutchings et al, 2003;Kube et al, 2003;Smith et al, 1998;Yang et al, 2001;Ye et al, 2001), and pathogen detection (Jenison et al, 2001;Earley et al, 2002;Dunbar et al, 2003;Cowan et al, 2004). The flexibility of this technology along with its inherent advantages suggests that additional applications will be forthcoming.…”

“…Initial efforts to categorize immune responses and the functional status of the immune system focused on two opposing, polarized classes of immune responses, T helper type 1 (Th1) and type 2 (Th2), defined in part by their associated cytokine expression profiles. It is now clear that the balance of the immune system can be established anywhere along a continuum spanning the entire spectrum from Th1 to Th2 (Chaouat et al, 2003;Kidd, 2003;Raghupathy, 2001;Chtanova and Mackay, 2001). Additionally, it has recently become clear that a multitude of physiologic processes, some involving other non-immune systems such as the nervous and endocrine systems, can impact the balance or status of the immune system (Elenkov and Chrousos, 2002;von Hertzen, 2002;Schwarz et al, 2001;Besedovsky and del Rey, 2000;Ader et al, 2001;Piccinni et al, 2000;Kovalovsky et al, 2000;Marshall and Agarwal, 2000;Becher et al, 2000).…”