Rapid and quantitative detection of tear MMP-9 for dry eye patients using a novel silicon nanowire-based biosensor

Lu, Zicheng; Liu, Tingting; Zhou, Xujiao; Yang, Yi; Liu, Yanxiang; Zhou, Hong; Wei, Shanhong; Zhai, Zimeng; Wu, Yuqing; Sun, Fengrui; Wang, Zifei; Li, Tie; Hong, Jiaxu

doi:10.1016/j.bios.2022.114498

Cited by 22 publications

(14 citation statements)

References 43 publications

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“…As for sensing TNT, a few electrons were transmitted from the peptide functional group to TNT, causing the value of negative gate voltage on the SiNW surface to decrease. 23 As a result, the degree of band bending shifts, and the concentration of accumulated holes in the nanowire decreases.…”

Section: Resultsmentioning

confidence: 99%

A highly sensitive and selective detection of 2,4,6-trinitrotoluene (TNT) using a peptide-functionalized silicon nanowire array sensor

Liu

Zhang²,

Huang³

et al. 2023

Anal. Methods

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Section: Resultsmentioning

confidence: 99%

A highly sensitive and selective detection of 2,4,6-trinitrotoluene (TNT) using a peptide-functionalized silicon nanowire array sensor

Liu

Zhang²,

Huang³

et al. 2023

Anal. Methods

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“…ECM-modulating enzymes such as matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) have been well characterized with respect to their role in DED. [ 79 , 80 ] Increased levels of MMPs, driven by inflammation are an indication of excessive ECM remodulation, which leads to epithelial cell loss,[ 24 , 30 , 52 ]which eventually leads to a loss of epithelial layer integrity. MMP9 has not only been suggested to be the biomarker for DED but MMP9 levels along with tear osmolarity have been found to be indicative of disease severity.…”

Section: Enzymesmentioning

confidence: 99%

Tear biomarkers in dry eye disease: Progress in the last decade

Kumar

Machiraju

Narasimhan

et al. 2023

Indian Journal of Ophthalmology

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Dry eye disease (DED) is a commonly occurring, multifactorial disease characterized by reduced tear film stability and hyperosmolarity at the ocular surface, leading to discomfort and visual compromise. DED is driven by chronic inflammation and its pathogenesis involves multiple ocular surface structures such as the cornea, conjunctiva, lacrimal glands, and meibomian glands. The tear film secretion and its composition are regulated by the ocular surface in orchestration with the environment and bodily cues. Thus, any dysregulation in ocular surface homeostasis causes an increase in tear break-up time (TBUT), osmolarity changes, and reduction in tear film volume, all of which are indicators of DED. Tear film abnormalities are perpetuated by underlying inflammatory signaling and secretion of inflammatory factors, leading to the recruitment of immune cells and clinical pathology. Tear-soluble factors such as cytokines and chemokines are the best surrogate markers of disease severity and can also drive the altered profile of ocular surface cells contributing to the disease. Soluble factors can thus help in disease classification and planning treatment strategies. Our analysis suggests increased levels of cytokines namely interleukin-1β (IL-1β), IL-2, IL-4, IL-6, IL-9, IL-12, IL-17A, interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α); chemokines (CCL2, CCL3, CCL4, CXCL8); MMP-9, FGF, VEGF-A; soluble receptors (sICAM-1, sTNFR1), neurotrophic factors (NGF, substance P, serotonin) and IL1RA and reduced levels of IL-7, IL-17F, CXCL1, CXCL10, EGF and lactoferrin in DED. Due to the non-invasive sample collection and ease of quantitively measuring soluble factors, tears are one of the best-studied biological samples to molecularly stratify DED patients and monitor their response to therapy. In this review, we evaluate and summarize the soluble factors profiles in DED patients from the studies conducted over the past decade and across various patient groups and etiologies. The use of biomarker testing in clinical settings will aid in the advancement of personalized medicine and represents the next step in managing DED.

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“…For example, Lu et al developed a uniform and stable silicon nanowire-based (SiNW) FET using an improved controllable process to detect MMP-9 levels in standard and human tear samples. [112] The antibody was attached to SiNW through a CHO and NH 2 reaction, and was used to capture MMP-9 in tear samples. The use of these SiNW devices was demonstrated for the detection of dry eye with human tear samples.…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

Recent Development and Applications of Sensors for the Detection of Matrix Metalloproteinases

Zhang

et al. 2023

Adv Materials Technologies

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of ECM cleavage. As the number of identified MMPs types increased, researchers began to classify the MMPs according to the protein structure. [3] Despite some structural differences, most of the structural modules of MMPs are still conservative, including i) an amino-terminal peptide which can leads MMPs to the endoplasmic reticulum, ii) a propeptide region (companying with a zinc-interacting thiol group) and a zinc-binding site which can maintains MMPs as inactive zymogens or as a catalytic domain, iii) a linker region of varying residue length that may determine the substrates to which the MMPs can accommodate, and iv) a hemopexin-like domain. [4] With the application of engineered genetic models these years, more biological roles of MMPs have been revealed in recently years. For example, through investigating MMPs' substrates of other proteins, such as pro-proteases, chemokines, antimicrobial peptides, cell surface proteins, coagulation factors, adhesion molecules, cryptic growth factors, and cytokines, [5] it has been revealed that the MMPs have a broad and dramatic impact on physiological processes, for example, immunity, [6] tissue repair, [7,8] cell differentiation, [9,10] and cell invasion. [11] In these physiological processes, MMPs are secreted as zymogens. [12] The cysteine (Cys) residues of MMPs' propeptide regions are able to interact with Zn 2+ of the catalytic domains. The formation of the thiols-Zn 2+ bonds play important roles in maintaining the inactivity of MMPs, i.e., MMPs' activations are achieved by breaking these bonds. It is conceivable that MMPs can be activated by a variety of endogenous or exogenous substances, such as mercurial compounds and chaotropic agents. [4] To regulate the activity of MMPs, organisms have evolved a family of two-domain proteins (TIMPs) as the endogenous inhibitors. [13] Most MMPs are ubiquitously secreted in mammalian organisms. The level of MMPs' expression is generally low under normal physiological conditions, while the abnormally elevated expression and activity levels are often associated with diseases. For example, as of the positive roles of MMPs in tumor growth and angiogenesis, [14] recent studies revealed that MMPs overexpression is implicated in a variety of cancers. [15] MMP-2 and MMP-9 are overexpressed in breast cancer, [16] cervical cancer, [17] etc., while MMP-7 are correlated with colorectal cancer (CRC). [18] Moreover, the prognostic value of MMPs has also been well demonstrated in treatment of different cancers. [19,20] For example, patients with high levels of MMP-2 and MMP-9 were clinically defined as a poor prognosis. [21] Therefore, MMPs' Matrix metalloproteinases (MMPs) are a class of zinc-dependent endopeptidases that play important roles in disease progression. High expression of MMPs is associated with various diseases, including cancer, cardiovascular diseases, neurological diseases, and inflammatory diseases. For better understanding the roles of MMPs in these diseases' development and the potential in early diagnosis and treatment m...

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Rapid and quantitative detection of tear MMP-9 for dry eye patients using a novel silicon nanowire-based biosensor

Cited by 22 publications

References 43 publications

A highly sensitive and selective detection of 2,4,6-trinitrotoluene (TNT) using a peptide-functionalized silicon nanowire array sensor

A highly sensitive and selective detection of 2,4,6-trinitrotoluene (TNT) using a peptide-functionalized silicon nanowire array sensor

Tear biomarkers in dry eye disease: Progress in the last decade

Recent Development and Applications of Sensors for the Detection of Matrix Metalloproteinases

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