Rapid and nondestructive determination of seed oil by pulsed nuclear magnetic resonance technique

Abstract

The pulsed NMR technique for rapid and nondestructive determination of oil in oilseeds has been developed. The effects of spin‐lattice relaxation time, spin‐spin relaxation time, seed moisture, angular position of the seeds, sample tube thickness, and sample height upon the magnitude and reproducibility of the NMR signal were studied. Based upon these studies, various parameters for seed oil analysis have been fixed. The oil content of Brassica, peanut, and sunflower seeds was determined. The reproducibility of the measurement is ± 1 %. The technique was tested by measuring the oil content of the same seeds by the cold percolation method (CCl4 extraction). It was further tested by determining the oil content of 60 Brassica seed samples independently at three laboratories. The results of these tests are given.

Study of Heterosis and Pollen Fertility in CGMS Based Pigeonpea [Cajanus cajan (L.) Millspaugh] Hybrids

“…Days to flower (DF) on the basis of 50 % plants of each genotype flowered, days to maturity (DM) on the basis of 80 % plants of each genotype matured were recorded. The protein content (PC) was estimated in percentage by using Nuclear Magnetic Resonance Technique (Tiwari et al, 1974). Harvest Index was calculated by using following formula [(Economic yield/Biological yield) x 100].…”

Components of Genetic Variation and Graphical Analysis (Wr-Vr) in Indian Mustard (Brassica juncea L. Czern & Coss.)

“…The data was recorded on five randomly selected plants of each genotypes per replication and the mean data calculate from it for different traits viz., plant height, number of branches per plant, number of siliquae per plant, days to flowering, days to maturity, seed yield per plant, harvest index, 1000-seed weight, oil content and fatty acid composition. Oil content of each sample was estimated in percentage by using Nuclear Magnetic Resonance Technique (Tiwari et al, 1974), while fatty acids composition of each sample was estimated in percentage by using Fourier Transferable Near Infrared (FT-NIR) Technique. The material under present investigation was tested for the agreement with assumptions basic to Hayman diallel analysis (Hayman, 1954a).…”

Weather, Quality Parameters and Yield of Rainfed Sunflower (Helianthus annuus L.) under Integrated Nutrient Management in Alfisols

“…The oil content of seed for each treatment was determined by using continuous type pulsed Nuclear Magnetic Resonance (NMR -oxford MQC) as suggested by Tiwari et al, (1974) and thereby oil percent in the seed was recorded directly. By multiplying oil content with the seed yield ha -1 oil yield (kg ha -1 ) was estimated.…”