Rapid and Non-destructive Detection and Identification of Two Strains of Wolbachia in Aedes aegypti by Near-Infrared Spectroscopy

Sikulu-Lord, Maggy T.; Maia, Marta; Milali, Masabho P.; Henry, Michael; Mkandawile, Gustav; Kho, Elise A.; Wirtz, Robert A.; Hugo, Leon E.; Dowell, Floyd E.; Devine, Gregor J.

doi:10.1371/journal.pntd.0004759

Cited by 65 publications

(70 citation statements)

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“…aegypti colony was established (at the insectory facilities of the QIMR Berghofer Medical Research Institute) from eggs of Wolbachia -free adult females collected at Innisfail, Australia, in April 2016. Mosquitoes were reared as previously described (48). Adult 3- to 4-day-old A.…”

Section: Methodsmentioning

confidence: 99%

De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case

Setoh

Prow

Peng

et al. 2017

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The major complications of an ongoing Zika virus outbreak in the Americas and Asia are congenital defects caused by the virus’s ability to cross the placenta and infect the fetal brain. The ability to generate molecular tools to analyze viral isolates from the current outbreak is essential for furthering our understanding of how these viruses cause congenital defects. The majority of existing viral isolates and infectious cDNA clones generated from them have undergone various numbers of passages in cell culture and/or suckling mice, which is likely to result in the accumulation of adaptive mutations that may affect viral properties. The approach described herein allows rapid generation of new, fully functional Zika virus isolates directly from deep sequencing data from virus-infected tissues without the need for prior virus passaging and for the generation and propagation of full-length cDNA clones. The approach should be applicable to other medically important flaviviruses and perhaps other positive-strand RNA viruses.

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Section: Methodsmentioning

confidence: 99%

De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case

Setoh

Prow

Peng

et al. 2017

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“…Differences in NIR spectra have been used to distinguish young (e.g. <7 days old) from older (7+ days old) malaria vectors, to identify morphologically identical Anopheles sibling species, and to detect the presence of the endosymbiont Wolbachia in Aedes aegypti mosquitoes [10–12]. Most recently, NIRS has been used to detect rodent malaria infections in laboratory-reared Anopheles stephensi mosquitoes [22] and Zika virus in Ae.…”

Section: Introductionmentioning

confidence: 99%

Detection of malaria in insectary-reared Anopheles gambiae using near-infrared spectroscopy

Maia

Kapulu

Muthui

et al. 2019

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23Large-scale surveillance of mosquito populations is crucial to assess the intensity of vector-borne 24 disease transmission and the impact of control interventions. However, there is a lack of accurate, cost-25 effective and high-throughput tools for mass-screening of vectors. This study demonstrates proof-of-26 concept that near-infrared spectroscopy (NIRS) is capable of rapidly identifying laboratory strains of 27 human malaria infection in African mosquito vectors. By using partial least square regression models 28 based on malaria-infected and uninfected Anopheles gambiae mosquitoes, we showed that NIRS can 29 detect oocyst-and sporozoite-stage Plasmodium falciparum infections with 88% and 95% accuracy, 30 respectively. Accurate, low-cost, reagent-free screening of mosquito populations enabled by NIRS 31 could revolutionize surveillance and elimination strategies for the most important human malaria 32 parasite in its primary African vector species. Further research is needed to evaluate how the method 33 performs in the field following adjustments in the training datasets to include data from wild-caught 34 infected and uninfected mosquitoes. 35 36 Introduction 37 Malaria is holding back development in endemic countries and remains one of the leading causes of 38 death in children under 5 years-old in sub-Saharan Africa [1-3]. During the past decade, the large-scale 39 roll-out of long-lasting insecticide treated nets and indoor residual spraying across Africa has resulted in 40 a substantial reduction in malaria cases [4]. The WHO's Global Technical Strategy for Malaria 2016-41 2030 seeks to reduce malaria incidence and related mortality by at least 90% and to eliminate the 42 disease in a minimum of 35 countries [1]. These bold goals will require new interventions that can 43 address residual malaria transmission as well as new tools to better monitor their impact on vector-44 borne disease transmission. Mosquito surveillance is a cornerstone of the control of malaria and other 45vector-borne diseases [5]. However, presently, there is no high-throughput, cost-efficient method to 46 identify Plasmodium infection and infectiousness in mosquitoes. Molecular methods such as ELISA and 47PCR are used to determine parasite infection, but these are expensive and laborious [6][7][8], challenging 48 resource-poor countries with few funds and limited access to reagents and equipment, and thus are 49 unsuitable for large-scale surveillance. A further complication is that typically only 1-2% of mosquitoes 50 may be infected with transmission stage parasites (sporozoites), meaning that very large sample sizes 51 must be tested to accurately quantify site and time-specific estimates of mosquito infection rates as will 52 be required to assess progress towards malaria elimination [9]. 54Recent advances indicate several mosquito traits can be accurately identified through analysis of their 55 tissues with near infrared spectroscopy (NIRS) [10][11][12][13] . Here, visible and NIR light (wavelength 400-56 2500 nanometers) ...

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“…The above approaches require destruction of specimens for DNA isolation or for tissue fixation. As a rapid and non-destructive alternative, Near-Infrared Spectroscopy (NIR) has been developed for identification of specimens infected with Wolbachia , including the distinction of two different Wolbachia strains [106]. This method, however, requires standardization according to species, sex, age, or any other condition that may affect absorbance, and is not 100% efficient.…”

Section: Methods To Study Wolbachiamentioning

confidence: 99%

Wolbachia pipientisassociated to tephritid fruit fly pests: from basic research to applications

Mateos

Martínez

Lanzavecchia

et al. 2018

Preprint

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23hominivorax Coquerel); several species of tsetse fly (Glossina spp.); and the codling 71 moth (Cydia pomonella L.) [reviewed in 17, 18]. 72 73 Successful SIT programs as part of Area-wide Integrated Pest Management (IPM) 74 strategies have also been implemented for several tephritids: Ceratitis capitata 75 Wiedemann; Anastrepha ludens Loew; Anastrepha obliqua Macquart; Anastrepha 76 fraterculus Wiedemann; Zeugodacus cucurbitae Coquillett; Bactrocera dorsalis Hendel; 77and Bactrocera tryoni Froggatt [6, 12, 13, 15]. SIT is currently being developed for two 78 additional tephritid species: Dacus ciliatus Loew and Bactrocera tau Walker [19, 20]. 79The advantages of the SIT over other pest control approaches (e.g. use of pesticides) 80 are that it is the most environmentally friendly and resistance is unlikely to evolve [21, 81 22]. 82 83 Another autocidal strategy where mating between mass-reared and wild insects can be 84 used to suppress pest populations is the incompatible insect technique (IIT). IIT also 85 relies on the principle of reducing female fertility, but utilizes endosymbiotic bacteria 86 instead of radiation, to induce a context-dependent sterility in wild females. It is based 87 on the ability of certain maternally inherited bacteria (namely from the genus Wolbachia) 88to induce a form of reproductive incompatibility known as cytoplasmic incompatibility (CI; 89 explained in the section below). Herein we review the current knowledge on taxonomic 90 diversity of Wolbachia-tephritid associations and their phenotypic consequences, and 91 identify gaps in knowledge and approaches in the context of potential application of IIT in 92 AW-IPM programs to control tephritid pests. We also discuss scenarios where these 93 two autocidal strategies, SIT and IIT, could be potentially combined for the population 94 suppression of tephritid pests. 95 96 5 The influence of Wolbachia on host ecology 97Insects and other arthropods are common hosts of maternally inherited bacteria 98 [reviewed in 23]. These heritable endosymbionts can have a strong influence on host 99 ecology. Such vertically transmitted bacteria are typically vastly (or fully) dependent on 100 the host for survival and transmission. Certain associations are obligate for both 101 partners, and generally involve a nutritional benefit to the host. Other heritable bacteria 102 are facultative, with such associations ranging from mutualistic to parasitic from the 103 host's perspective. Among these, Wolbachia is the most common and widespread 104 facultative symbiont of insects and arthropods [24][25][26][27]. 105 106 Wolbachia is a diverse and old genus [possibly older than 200 million years; 28] of 107 intracellular gram-negative Alphaproteobacteria (within the order Rickettsiales) 108 associated with arthropods and filarial nematodes. Wolbachia cells resemble small 109 spheres 0.2-1.5 μ m, occur in all tissue types, but tend to be more prevalent in ovaries 110 and testicles of infected hosts, and are closely associated with the female germline 111 [...

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Rapid and Non-destructive Detection and Identification of Two Strains of Wolbachia in Aedes aegypti by Near-Infrared Spectroscopy

Cited by 65 publications

References 55 publications

De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case

De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case

Detection of malaria in insectary-reared Anopheles gambiae using near-infrared spectroscopy

Wolbachia pipientisassociated to tephritid fruit fly pests: from basic research to applications

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