Rapid and low cost replication of complex microfluidic structures with PDMS double casting technology

Yang, Luxia; Hao, Xiaojian; Wang, Chunshui; Zhang, Binzhen; Wang, Wanjun

doi:10.1007/s00542-013-2004-8

Cited by 31 publications

(26 citation statements)

References 32 publications

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“…55 Initially, to produce the polydimethylsiloxane (PDMS) leaf imprints (Figure 1b) we followed standard PDMS double-casting protocols, which have also been used to produce leaf replicas. 42,45,46,50,56 However, the high temperatures (~ 80 °C ) required for curing the PDMS to produce the imprint, resulted in the A. thaliana leaf samples degrading. Thus, having a dramatic effect on the quality of the imprint.…”

Section: Resultsmentioning

confidence: 99%

“…The imprint was then rinsed with deionised water, and subsequently dried with nitrogen. 45,46 Alternatively, (2) treatment with trichloro(1H,1H,2H,2H-perfluorooctyl)silane (448931, Sigma-Aldrich), can be undertaken.…”

Section: Anti-adhesionmentioning

confidence: 99%

“…23,42 A ratio of 10:1 is also commonly used in other doublecasting applications, such as bioimprinting. [44][45][46] Prior to casting the leaf imprint the A. thaliana samples were briefly dried to remove the surface moisture of the leaves. This was done to ensure the PDMS cures on the surface.…”

Section: Introductionmentioning

confidence: 99%

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Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology

Soffe

Bernach

Remus-Emsermann

et al. 2019

Preprint

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words)Artificial surfaces are commonly used in place of leaves in phyllosphere microbiology to study microbial behaviour on plant leaf surfaces. Studies looking into individual environmental factors influencing microorganisms are routinely carried out using artificial surfaces.Commonly used artificial surfaces include nutrient agar, isolated leaf cuticles, and reconstituted leaf waxes. However, interest is growing in using microstructured surfaces mimicking the complex topography of leaf surfaces for phyllosphere microbiology. As such replica leaf surfaces, produced by microfabrication, are appearing in literature. Replica leaf surfaces have been produced in agar, epoxy, polystyrene, and polydimethylsiloxane (PDMS). However, these protocols are not suitable for replicating fragile leaves such as of the model plant Arabidopsis thaliana. This is of importance as A. thaliana is a model system for molecular plant genetics, molecular plant biology, and microbial ecology. Here we present a versatile replication protocol for replicating fragile leaf surfaces into PDMS. We display the capacity of our replication process using optical microscopy, atomic force microscopy (AFM), and contact angle measurements to compare living and PDMS replica A. thaliana leaf surfaces. To highlight the use of our replica leaf surfaces for phyllosphere microbiology, we visualised bacteria on the replica leaf surfaces in comparison to living leaf surfaces.

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Section: Resultsmentioning

confidence: 99%

Section: Anti-adhesionmentioning

confidence: 99%

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Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology

Soffe

Bernach

Remus-Emsermann

et al. 2019

Preprint

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“…However, these are expensive and complex for multi-level high-aspect-ratio microstructures. SU-8 negative resist masters represent a less expensive alternative to silicon, but the fabrication of SU-8 molds with negative microstructures (deep cavities) is difficult due to lack of exchange of fresh SU-8 developer in micro-cavities [26].…”

Section: Resultsmentioning

confidence: 99%

Fabrication of In-Channel High-Aspect Ratio Sensing Pillars for Protrusive Force Measurements on Fungi and Oomycetes

Sun

Tayagui

Garrill

et al. 2018

J. Microelectromech. Syst.

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This paper reports the fabrication and application of a Lab-on-a-Chip platform containing single-elastomeric micropillars in channel constrictions, which enable the measurement of protrusive forces exerted by individual fungal hyphae. We show the device design, the fabrication process, and photoresist optimization required to adapt the microfluidic platform to relatively thin hyphae. To demonstrate the applicability of the devices, the oomycete Achlya bisexualis and the fungus Neurospora crassa were cultured on PDMS chips. Devices were combined with confocal imaging to study the interaction of A. bisexualis hyphae with the measurement pillars. The force exerted by individual hyphae of N. crassa was measured and compared with a hyphal growth rate and diameter. The platform provides a new tool to help understand the molecular processes that underlie protrusive growth and this may present new ways to tackle the pathogenic growth of these organisms and thus combat the loss of diversity that they cause. This paper is based on the conference proceedings presented at the 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018), Belfast. [2018-0090] Index Terms-Lab-on-a-chip, force sensor, PDMS micropillars, fungi and oomycetes.

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“…Both polarities can be transferred into casein using PDMS double-casting, which has been found to generate average replication errors of less than 4%. 27 Once the PDMS mould is obtained, repeated cleaning of the replica with 10% SDS/HCl and 1% trypsin-EDTA was used to ensure that no biological material remains on the mould and only topographical information is transferred. Figure 6(c) shows the final positive casein replica of the same area as in Figs.…”

Section: Replication Of Bioimprints Onto Casein Filmsmentioning

confidence: 99%

Fabrication of free-standing casein devices with micro- and nanostructured regular and bioimprinted surface features

Hashemi

Mutreja

Alkaisi

et al. 2015

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

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This work introduces a novel process for the fabrication of free-standing biodegradable casein devices with micro-and nanoscale regular and biomimetic surface features. Fabrication of intermediate polydimethylsiloxane (PDMS) moulds from photoresist masters and liquid-casting of casein is used to transfer arbitrary geometrical shapes onto the surface of casein devices. Casein film composition was optimized for mechanical stability and pattern resolution. It was found that 15% casein in 0.2% NaOH solution, mixed with 10% glycerol, and cross-linked by addition of 2% glutaraldehyde produced the best pattern transfer results. Biomimetic cell-like shapes were transferred onto casein by use of bioimprinting of two-dimensional cell-cultures into PDMS. To demonstrate this process, C2C12 mouse myoblasts were cultured on microscope slides, replicated into PDMS and casein using liquid casting and drying. Recessed alignment grids were integrated into the microscope glass slides to facilitate direct comparison of original cells and their bioimprints on PDMS and casein. Optical microscopy and atomic force microscopy confirmed the transfer of micron-scale morphological features, such as cell outlines, nuclei and larger lamellipodia, into the casein surface. Nanoscale feature resolution in casein was found to be limited compared to the PDMS intermediate moulds, which was attributed to limited wetting of the aqueous casein solution. Strategies to increase resolution of the casein transfer step, as well as degradation behavior of the fabricated devices in cell culture media are currently underway. Substrates fabricated with this process have applications in stem cell engineering, regenerative medicine, and implantable devices.

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Rapid and low cost replication of complex microfluidic structures with PDMS double casting technology

Cited by 31 publications

References 32 publications

Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology

Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology

Fabrication of In-Channel High-Aspect Ratio Sensing Pillars for Protrusive Force Measurements on Fungi and Oomycetes

Fabrication of free-standing casein devices with micro- and nanostructured regular and bioimprinted surface features

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