Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus

Hou, Han Wei; Petchakup, Chayakorn; Tay, Hui Min; Tam, Zhi Yang; Dalan, Rinkoo; Chew, Daniel Ek Kwang; Li, King Ho Holden; Boehm, Bernhard O.

doi:10.1038/srep29410

Cited by 54 publications

(56 citation statements)

References 62 publications

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“…Further, blood monocytes and neutrophils are sentinel cells that drive inflammation and concomitant pro-inflammatory cytokine production in vivo 3 , 9 . Many studies have also shown that the gene expression and phenotypic profiles of monocytes and neutrophils are altered in response to glucose and in a T2DM milieu 9 – 13 . Given this, we opted to study whether alterations in inflammatory gene expression occurred in both monocytes and neutrophils in the context of T2DM.…”

Section: Introductionmentioning

confidence: 99%

Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications

Gupta

Maratha

Siednienko

et al. 2017

Sci Rep

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The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as Toll-like receptors (TLRs) and inflammatory mediators such as IL-6, TNFα and IL-1β have been linked with T2DM. Herein, we sought to establish whether patients with T2DM and underlying complications exhibit perturbations in cytokine and TLR expression. Serum cytokine and mRNA levels of cytokines/TLRs in monocytes (M) and neutrophils (N) were measured in a cohort of 112 diabetic patients: good glycaemic control without complications (GC), good glycaemic control with complications (GCC), poor glycaemic control without complications (PC) and poor glycaemic control with complications (PCC) and compared them with 34 non-diabetic volunteers (NGT). Serum cytokine levels were normal in all study participants. In the GC group, cytokine and TLR gene expression were enhanced compared to NGT. In contrast, suppressed cytokine and TLR gene expression were evident in PC, GCC & PCC groups when compared to the GC. In conclusion, whereas serum pro-inflammatory cytokine levels are unaltered in T2DM patients, differences in inflammatory gene profiles exist among the T2DM patient groups.

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Section: Introductionmentioning

confidence: 99%

Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications

Gupta

Maratha

Siednienko

et al. 2017

Sci Rep

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show abstract

“…Likewise, the NLRP3 inflammasome is also activated during hypertension [ 222 ]. Interestingly, the gene expression profiles of cultured peripheral immune cells have been shown to change in response to high glucose conditions and T2DM, affecting neutrophil movement [ 223 ], increasing monocyte activation markers [ 224 ] and increasing monocytic TLR expression [ 225 ]. Monocytes and neutrophils from T2DM patients have also been found to have increased pro-inflammatory cytokine and TLR expression, with the extent of this effect changing based on glycemic control [ 226 ].…”

Section: The Metabolic Disorders and Admentioning

confidence: 99%

Inflammation: the link between comorbidities, genetics, and Alzheimer’s disease

Newcombe

Camats-Perna

Silva

et al. 2018

J Neuroinflammation

390

302

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Alzheimer’s disease (AD) is a neurodegenerative disorder, most cases of which lack a clear causative event. This has made the disease difficult to characterize and, thus, diagnose. Although some cases are genetically linked, there are many diseases and lifestyle factors that can lead to an increased risk of developing AD, including traumatic brain injury, diabetes, hypertension, obesity, and other metabolic syndromes, in addition to aging. Identifying common factors and trends between these conditions could enhance our understanding of AD and lead to the development of more effective treatments. Although the immune system is one of the body’s key defense mechanisms, chronic inflammation has been increasingly linked with several age-related diseases. Moreover, it is now well accepted that chronic inflammation has an important role in the onset and progression of AD. In this review, the different inflammatory signals associated with AD and its risk factors will be outlined to demonstrate how chronic inflammation may be influencing individual susceptibility to AD. Our goal is to bring attention to potential shared signals presented by the immune system during different conditions that could lead to the development of successful treatments.

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“…Quantitative tools are required to monitor these features. Morphology, as observed by light microscopy is a label-free and easily accessible phenotype (3). However engagement of surface markers has been shown to influence cell fate (2).…”

Section: Introductionmentioning

confidence: 99%

“…However engagement of surface markers has been shown to influence cell fate (2). Morphology, as observed by light microscopy is a label-free and easily accessible phenotype (3). Moreover it is suitable for long-term follow-up.…”

Section: Introductionmentioning

confidence: 99%

Combination of imaging flow cytometry and time‐lapse microscopy for the study of label‐free morphology dynamics of hematopoietic cells

et al. 2017

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Cell differentiation is a longitudinal and dynamic process. Studying and quantifying such a process require tools combining precise time resolution and statistical power. Imaging flow cytometry (IFC) provides statistically significant number of microscopy images of individual cells in a sample at a given time point. Time-lapse microscopy (TLM) is the method of choice for studying the dynamics of cell processes at a high temporal, but low statistical resolution. In this work, we show that the dynamic changes of cord-blood derived CD34+ cells in response to cytokine stimulation can be successfully studied, in a label-free way, by the combination of the IFCs statistical power and the TLM's high time resolution. Cell morphology phenotypes were quantified through roundness and surface area, measured both in IFC and with a homemade segmentation algorithm in TLM. Two distinct morphologies-polarized and round-were observed in cord-blood derived CD34+. We show that some cells have the ability to fluctuate between these morphologies, suggesting that the apparent stable composition of round and polarized cells may actually represent a dynamic equilibrium. This example demonstrates that the different resolutions and modalities of IFC and TLM are complementary and allow the study of complex dynamic biological processes. © 2017 International Society for Advancement of Cytometry.

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Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus

Cited by 54 publications

References 62 publications

Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications

Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications

Inflammation: the link between comorbidities, genetics, and Alzheimer’s disease

Combination of imaging flow cytometry and time‐lapse microscopy for the study of label‐free morphology dynamics of hematopoietic cells

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