Rapid and label-free bioanalytical method of alpha fetoprotein detection using LSPR chip

Kim, Dongjoo; Kim, Jinwoon; Kwak, Cheol Hwan; Heo, Nam Su; Oh, Sejong; Lee, Hoomin; Lee, Go-Woon; Vilian, A.T. Ezhil; Han, Young‐Kyu; Kim, Woo‐Sik; Kim, Gi Bum; Kwon, Soonjo; Huh, Yun Suk

doi:10.1016/j.jcrysgro.2016.09.066

Cited by 17 publications

(3 citation statements)

References 22 publications

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“…Research is also being conducted on other types of nanoparticle-based sensing devices for bacterial pathogens, virus particles, hazardous chemicals, toxic proteins, peptides, and other entities ( Kim et al, 2016a , b ). Figure 3 represents the evolution of various kinds of liposome nanoparticles for their potential commercial usage.…”

Section: Liposome Nanoparticles As Detection Sensor In Food Sectormentioning

confidence: 99%

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

et al. 2017

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Safety of food is a noteworthy issue for consumers and the food industry. A number of complex challenges associated with food engineering and food industries, including quality food production and safety of the food through effective and feasible means can be explained by nanotechnology. However, nanoparticles have unique physicochemical properties compared to normal macroparticles of the same composition and thus could interact with living system in surprising ways to induce toxicity. Further, few toxicological/safety assessments have been performed on nanoparticles, thereby necessitating further research on oral exposure risk prior to their application to food. Liposome nanoparticles are viewed as attractive novel materials by the food and medical industries. For example, nanoencapsulation of bioactive food compounds is an emerging application of nanotechnology. In several food industrial practices, liposome nanoparticles have been utilized to improve flavoring and nutritional properties of food, and they have been examined for their capacity to encapsulate natural metabolites that may help to protect the food from spoilage and degradation. This review focuses on ongoing advancements in the application of liposomes for food and pharma sector.

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Section: Liposome Nanoparticles As Detection Sensor In Food Sectormentioning

confidence: 99%

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

et al. 2017

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“…This characteristic can be used to prepare LSPR sensors for biological and chemical detection. LSPR biosensors can detect low-concentration cancer markers and provide a reference for auxiliary diagnosis, condition monitoring, and efficacy evaluation. − The LSPR sensor based on Au NPs has been used to realize the rapid detection of low-concentration AFP, CEA, and other cancer markers. − Recently, Lee et al developed a multiplex cancer marker biosensor by immobilizing chemically synthesized plasmonic nanoparticles . Aćimović et al demonstrated a nanoplasmonic biosensor of Au nanorods with eight independent microfluidic channels by the standard electron beam lithography (EBL)–reactive ion etching (RIE) process to achieve 50% human serum cancer marker detection .…”

Section: Introductionmentioning

confidence: 99%

Au Nanoparticle-Based Integrated Microfluidic Plasmonic Chips for the Detection of Carcinoembryonic Antigen in Human Serum

Fang

et al. 2022

ACS Appl. Nano Mater.

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A nanoplasmonic biosensor with merits of label-free and rapid detection provides a key tool for the detection of cancer markers in ultralow concentrations. Herein, a localized surface plasmon resonance (LSPR) biosensor integrated with microfluidics, which has multiple sensing units, was developed by nanomaterial fabrication technology. The performance of the LSPR biosensor was experimentally explored using nine clinical sample tests after the biofunctionalization of Au nanoparticles by biomodification and verified by four characterization methods. The linear detection response of carcinoembryonic antigen (CEA) appeared in the range of 0.5−100 ng/mL, with a detection limit below 1 ng/mL. The results illustrated that the LSPR platform improves the signal-to-noise ratio by about 85 times after adjusting testing system parameters. Furthermore, the simultaneous detection results of the proposed biosensor in nine serum samples of 50 μL from breast cancer patients were proved to be consistent with enzyme-linked immunosorbent assay (ELISA) results (r = 0.94). The low cost and remarkable performance of this LSPR biosensor make it promising to develop a precise clinical detection platform for auxiliary diagnosis, condition monitoring, and efficacy evaluation of cancer.

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“…A conventional universal strategy for enhancing the detection sensitivity is to amplify output signals by introducing as many enzyme molecules as possible . Currently, immunoassays that measure protein biomarkers by means of specific recognition include enzyme-linked immunosorbent assays (ELISA), , colorimetric methods, − electrochemiluminescence assays, fluoroimmunoassays, , electrochemical immunosensor-based assays, , surface plasmon resonance-based assays , and photoelectrochemical-based immunoassays. , Despite ongoing efforts, the detection sensitivity of enzymes has been low due to their inferior stability and vulnerable catalytic activity, and consequently so has their limitation . The need for an effective enzyme-free signal amplification immunoassay has become increasingly urgent since this type of method enables ultrasensitive detection with rapid response and long-term stability, which are highly desirable for clinical diagnosis.…”

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confidence: 99%

Plasmon-Induced Photoreduction System Allows Ultrasensitive Detection of Disease Biomarkers by Silver-Mediated Immunoassay

Hua

Han³

et al. 2020

ACS Sens.

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Current strategies for the detection of disease biomarkers often require enzymatic assays that may have limited sensitivity due to inferior stability and vulnerable catalytic activity of the enzyme. A new enzyme-free amplification method for identifying suitable biomarkers is necessary to lower the limit of detection and improve many critical diagnosis applications. Here, we presented an enzyme-free amplified plasmonic immunoassay that enhanced the detection sensitivity of disease biomarkers by combining a novel plasmon-induced silver photoreduction system with a silver nanoparticle (AgNP)-linked immunoassay. The key step to achieving ultrasensitivity was to use Ag + from dissolved AgNPs that control the growth rate of the silver coating on plasmonic nanosensors under visible light illumination. We demonstrated the outstanding sensitivity and robustness of this assay by detecting the disease biomarker alpha-fetoprotein (AFP) at a low concentration of 3.3 fg mL −1 . The detection of AFP was further confirmed in the sera of hepatocellular carcinoma patients.

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Rapid and label-free bioanalytical method of alpha fetoprotein detection using LSPR chip

Cited by 17 publications

References 22 publications

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

Au Nanoparticle-Based Integrated Microfluidic Plasmonic Chips for the Detection of Carcinoembryonic Antigen in Human Serum

Plasmon-Induced Photoreduction System Allows Ultrasensitive Detection of Disease Biomarkers by Silver-Mediated Immunoassay

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