Rapid and highly sensitive high-performance liquid chromatographic method for the determination of histamine and 3-methylhistamine in biological samples using fluorescamine as the derivatizing agent

Separation assay of histamine and its metabolites in biological specimens

“…Intact HA and the metabolites without derivatization are simultaneously separated by a cation-exchange column (Yamatodani et al, 1985;Itoh et al, 1991;Mine et al, 1986;Houdi et al, 1987;Hermann et al, 1993). Although the other types of columns such as the octadesylsilyl column (ODS; Itoh et al, 1992;Allenmark et al, 1985;Ronnberg et al, 1984;Harsing et al, 1986a,b;Tsikas et al, 1993;Hui and Taylor, 1984;van Haaster et al, 1993;Saito et al, 1992;Skofitsch et al, 1981;Devalia et al, 1985;Kasziba et al, 1988) and the cyanopropylsilyl column (Siegel et al, 1990a,b) can be used for the separation, not only is the separation efficiency not very high, but also peak tailing based on adsorption by the resins sometimes occurs. In such a case, an ion-pair reagent (usually acidic compound) is commonly added to the mobile-phase (Vind et al, 1991).…”

“…As other pre-column derivatization reagents, fluorescamine (van Haaster et al, 1993(van Haaster et al, , 1994, 4-BrMC (Tsuruta et al, 1987), DBD-F and DBD-PZ (Toyo'oka et al, 2004;Kawanishi et al, 2006Kawanishi et al, , 2007 are also applicable for the determination of HA and related compounds (MHA, MIAA, IAA, etc.). The samples in coronary perfusates, extracts of homogenized rat hearts and supernatants of stimulated peritoneal mast cells were purified and concentrated on cation-exchange columns and derivatized with fluorescamine in borate buffer (pH 9.1) at 40°C for 1 min (van Haaster et al, 1993).…”

“…The separated HA and the metabolites, in spite of the derivatives and non-derivatives, are determined by several detectors such as ultraviolet-visible (UV-vis; Hui and Taylor, 1984), FL (Yamatodani et al, 1985;Itoh et al, 1991Itoh et al, , 1992Allenmark et al, 1985;Ronnberg et al, 1984;Harsing et al, 1986a;Tsikas et al, 1993;van Haaster et al, 1993;Saito et al, 1992;Skofitsch et al, 1981;Devalia et al, 1985;Kasziba et al, 1988;Siegel et al, 1990a-c;Vind et al, 1991;Robert et al, 1983;Kitanaka et al, 2004), electrochemical (EC; Mine et al, 1986;Harsing et al, 1986a,b;Borycz et al, 2000;Han and Vohra, 1991), chemiluminescence (CL) and MS. The UV detector is widely used for the determination of various organic compounds.…”

“…Although FL detection is another sensitive determination method for HA and the metabolites, the labeling with an FL reagent is essential. The pre-, post-and oncolumn labeling of amino groups at the N α -and/or N τ -position(s) of HA and the metabolites are generally carried out by suitable FL reagents, such as orthophthalaldehyde (OPA)/thiol (Yamatodani et al, 1985;Itoh et al, 1991Itoh et al, , 1992Saito et al, 1992;Skofitsch et al, 1981;Devalia et al, 1985;Kasziba et al, 1988;Siegel et al, 1990a,b;Robert et al, 1983), fluorescamine (van Haaster et al, 1994), 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F; Toyo'oka et al, 2004;Kawanishi et al, 2006Kawanishi et al, , 2007 and 4-bromomethyl-7-methoxycoumarine (4-BrMC; Tsuruta et al, 1987). 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) is also adaptable for the labeling of the carboxylic acid in MIAA and IAA (Kawanishi et al, 2006).…”

Development of an automated on-line pre-column derivatization procedure for sensitive determination of histamine in food with high-performance liquid chromatography–fluorescence detection

“…As the reaction between OPA and histamine completes within 30 s in the presence of 2-mercaptoethanol at room temperature, pre-column derivatization with OPA is commonly adopted for HPLC-FLD analysis of histamine. However, critical conditions have to be controlled to eliminate the influence of the relative instability of the derivatives [27,28].…”