Rapid and Highly Sensitive Detection of Lead Ions in Drinking Water Based on a Strip Immunosensor

Abstract

In this study, we have first developed a rapid and sensitive strip immunosensor based on two heterogeneously-sized gold nanoparticles (Au NPs) probes for the detection of trace lead ions in drinking water. The sensitivity was 4-fold higher than that of the conventional LFA under the optimized conditions. The visual limit of detection (LOD) of the amplified method for qualitative detection lead ions was 2 ng/mL and the LOD for semi-quantitative detection could go down to 0.19 ng/mL using a scanning reader. The method suffered from no interference from other metal ions and could be used to detect trace lead ions in drinking water without sample enrichment. The recovery of the test samples ranged from 96% to 103%. As the detection method could be accomplished within 15 min, this method could be used as a potential tool for preliminary monitoring of lead contamination in drinking water.

Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples

“…Mouse spleen lymphocytes were fused with myeloma cells using polyethylene glycol (PEG) to form hybrid cells Kong et al, 2015;Kuang et al, 2013). One week after cell fusion, the ic-ELISA method was used to screen for positive cells with high anti-PEF activity.…”

Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples

“…Both of the conjugates were dialyzed against 0.01 M phosphate-buffered saline (PBS) for 3 d in the dark. The anti-AOH mAb was prepared in our laboratory and purified via the caprylic acidammonium sulfate precipitation method (Kuang et al, 2013). The mAb was characterized by indirect competitive ELISA (ic-ELISA), which is similar to conventional protocols .…”

Development of a monoclonal antibody assay and immunochromatographic test strip for the detection of amikacin residues in milk and eggs

“…Each of the five selected hybridoma cell lines was injected intraperitoneally into at least two mature female BALB/c mice (primed with paraffin oil) to produce ascites fluid. The ascites fluid was harvested 7-14 days after injection, then purified using the caprylic acid-ammonium sulfate precipitation method (Kuang et al, 2013) and dialyzed against PBS for 3 days before storage at −20°C.…”