Rapid and global detection and characterization of the constituents in ShengMai San by ultra‐performance liquid chromatography–high‐definition mass spectrometry

Abstract

An ultra-performance liquid chromatography-high definition mass spectrometry (UPLC-HDMS) method was developed for detection and characterization of the chemical constituents in ShengMai San (SMS), a traditional Chinese medical formula (TCMF). The full-scan LC-MS/MS data sets combined with extra mass were acquired within 14 min using UPLC-HDMS in the MS(E) mode in a single injection. As a result, 92 compounds were identified by comparing the accurate mass and fragments information with that of the authentic standards as well as by MS analysis and the correlative references data. These constituents included ginsenosides, lignans, steroidal saponins and homoisoflavanones. Among them, 25-hydroxyginsenosides were discovered in SMS for the first time. Compare with the previous studies, our research detected more compounds and presented more rapid by applying UPLC-HDMS. It is concluded that a rapid and effective method has been established based on UPLC-HDMS with the utilization of MS(E) , which shows high sensitivity and resolution that is suitable for identifying the constituents of SMS, and this method could be applied to other TCMF.

Simultaneous Determination of Isoflavones, Saponins and Flavones in Flos Puerariae by Ultra Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

“…Concerning saponins, only kaikasaponin III, kakkasaponin I and soyasaponin I were analyzed, 9,18) which possess hypoglycemic, hypolipidemic, and hepatoprotective activities. 12,23) It is known that saponins are one of the important components in Pueraria sp., 24) thus, chemical profiling and quantification of saponins are necessary for the quality control of the raw materials of Flos Puerariae as well.…”

Shengmai Formula suppressed over-activated Ras/MAPK pathway in C. elegans by opening mitochondrial permeability transition pore via regulating cyclophilin D

“…Baill.) (FS)1. It has been used for nearly 1,000 years in China, and is currently listed in the Chinese Pharmacopoeia mainly for treating coronary heart disease1234.…”

Dose-dependent targeted knockout methodology combined with deep structure elucidation strategies for Chinese licorice chemical profiling

“…Effluent collected in the first 5 min under 2% B and labeled as fraction 0 was used to flush the strong polar compounds, such as monosaccharides, polysaccharides and amino acids, to reduce interference in the MS and NMR signals. The remaining 20 fractions (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were collected from the p-HPLC every 3 min. Data were processed by ChemStation B.04.03 software.…”

“…Nowadays, TCM is attracting increasing attention worldwide due to its distinguished efficiency and minimal or negligible toxicity [1][2][3]. However, the disadvantages, including poor quality control, ambiguous pharmacodynamics and indefinable therapeutic mechanisms, limit the worldwide spread of TCM [4].…”