Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry

Majchrzykiewicz-Koehorst, J.A.; Heikens, E.; Trip, Hein; Hulst, A.G.; Jong, A.L. de; Viveen, Marco C.; Sedee, N. J. A.; Plas, J. van der; Coenjaerts, Frank E. J.; Paauw, Armand

doi:10.1016/j.jviromet.2014.11.014

Cited by 37 publications

(50 citation statements)

References 25 publications

(31 reference statements)

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“…At present, mass spectrometry has had limited application in clinical virology6232425. In this light, the main purpose of this study was the development of a new tool based on MALDI-TOF MS as a model to be applied to the identification of cultivable respiratory viruses after a cell culture step.…”

Section: Discussionmentioning

confidence: 99%

“…Despite many literature data about MALDI-TOF MS application in routine clinical microbiology, and in experimental approaches related to bacteria, parasites, yeasts and moulds616171819202122, very few reports are available about its application in virus research and laboratory diagnosis of viral infections6232425. In most of the studies, specific viral genome sequences are previously amplified by PCR and then the amplicons are analysed by MALDI-TOF MS15.…”

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confidence: 99%

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Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

Calderaro

Arcangeletti

Rodighiero

et al. 2016

Sci Rep

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In this study matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), a reliable identification method for the diagnosis of bacterial and fungal infections, is presented as an innovative tool to investigate the protein profile of cell cultures infected by the most common viruses causing respiratory tract infections in humans. MALDI-TOF MS was applied to the identification of influenza A and B viruses, adenovirus C species, parainfluenza virus types 1, 2 and 3, respiratory syncytial virus, echovirus, cytomegalovirus and metapneumovirus. In this study MALDI-TOF MS was proposed as a model to be applied to the identification of cultivable respiratory viruses using cell culture as a viral proteins enrichment method to the proteome profiling of virus infected and uninfected cell cultures. The reference virus strains and 58 viruses identified from respiratory samples of subjects with respiratory diseases positive for one of the above mentioned viral agents by cell culture were used for the in vitro infection of suitable cell cultures. The isolated viral particles, concentrated by ultracentrifugation, were used for subsequent protein extraction and their spectra profiles were generated by MALDI-TOF MS analysis. The newly created library allowed us to discriminate between uninfected and respiratory virus infected cell cultures.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

Calderaro

Arcangeletti

Rodighiero

et al. 2016

Sci Rep

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show abstract

“…From these peptide sequences, not only proteins of interest can be identified, also the micro organism itself can be specified to, at least, species level [16,17]. Recent developments in proteomic analysis using LC-MS/MS reduced the time to result, making it possible to analyze a sample within 6 h [18].…”

Section: Research Articlementioning

confidence: 99%

Identification of Microorganisms Grown in Blood Culture Flasks using Liquid Chromatography–Tandem Mass Spectrometry

et al. 2017

Self Cite

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Aim:Bloodstream infections are a common cause of disease and a fast and accurate identification of the causative agent or agents of bloodstream infections would aid the start of adequate treatment. Materials & methods: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics method was developed for the identification of bacterial species directly from blood cultures that were simulated by inoculating blood culture bottles with single or multiple clinically relevant microorganisms. Results: Using LC-MS/MS, the single species were correctly identified in 100% of the blood cultures, whereas for polymicrobial infections, 78% of both species were correctly identified in blood cultures. Conclusion: The LC-MS/MS method allows for the identification of the causative agent of positive blood cultures.

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“…The potential for clinical applications of MS for studying host cell interaction and analyzing biomarkers of the virus itself or linked to the host response has been reviewed elsewhere . The detection of viral proteins can be performed directly in various fluids or cells from infected patients . However, viral identification suffers from their highly evolutive nature.…”

Section: Emerging and Other Specific Approaches In The Environmentalmentioning

confidence: 99%

Mass spectrometry for the detection of bioterrorism agents: from environmental to clinical applications

Duriez¹,

Armengaud

Fenaille

et al. 2016

J. Mass Spectrom.

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In the current context of international conflicts and localized terrorist actions, there is unfortunately a permanent threat of attacks with unconventional warfare agents. Among these, biological agents such as toxins, microorganisms, and viruses deserve particular attention owing to their ease of production and dissemination. Mass spectrometry (MS)-based techniques for the detection and quantification of biological agents have a decisive role to play for countermeasures in a scenario of biological attacks. The application of MS to every field of both organic and macromolecular species has in recent years been revolutionized by the development of soft ionization techniques (MALDI and ESI), and by the continuous development of MS technologies (high resolution, accurate mass HR/AM instruments, novel analyzers, hybrid configurations). New possibilities have emerged for exquisite specific and sensitive detection of biological warfare agents. MS-based strategies for clinical application can now address a wide range of analytical questions mainly including issues related to the complexity of biological samples and their available volume. Multiplexed toxin detection, discovery of new markers through omics approaches, and identification of untargeted microbiological or of novel molecular targets are examples of applications. In this paper, we will present these technological advances along with the novel perspectives offered by omics approaches to clinical detection and follow-up.

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Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry

Cited by 37 publications

References 25 publications

Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

Identification of Microorganisms Grown in Blood Culture Flasks using Liquid Chromatography–Tandem Mass Spectrometry

Mass spectrometry for the detection of bioterrorism agents: from environmental to clinical applications

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