Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOG

Zhao, Hongxi; Yang, Li; Jin, Hai-feng; Xie, Li; Liu, Chuang; Jiang, Feng; Luo, Yunping; Guo, Yijia; Li, Yi; Wang, Jun; Li, Lingsong; Yao, Yuanqing; Wang, Xiaohong

doi:10.1016/j.diff.2010.03.002

Cited by 77 publications

(64 citation statements)

References 21 publications

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“…Kim et al first demonstrated that PSCs can be induced from neural progenitor cells with the use of only two of the reprogramming factors: exogenous OCT4 and either KLF4 or C-MYC [57]. Since then, other groups have also reported simplified reprogramming methods with other somatic cells, such as the conversion of human amnion-derived cells [58] and fetal hepatocytes [59] into iPSCs using only three reprogramming factors, OCT4, SOX2, and NANOG.…”

Section: Discussionmentioning

confidence: 99%

Direct Reprogramming of Human Primordial Germ Cells into Induced Pluripotent Stem Cells: Efficient Generation of Genetically Engineered Germ Cells

Bazley

Liu

Yuan

et al. 2015

Stem Cells and Development

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Primordial germ cells (PGCs) share many properties with embryonic stem cells (ESCs) and innately express several key pluripotency-controlling factors, including OCT4, NANOG, and LIN28. Therefore, PGCs may provide a simple and efficient model for studying somatic cell reprogramming to induced pluripotent stem cells (iPSCs), especially in determining the regulatory mechanisms that fundamentally define pluripotency. Here, we report a novel model of PGC reprogramming to generate iPSCs via transfection with SOX2 and OCT4 using integrative lentiviral. We also show the feasibility of using nonintegrative approaches for generating iPSC from PGCs using only these two factors. We show that human PGCs express endogenous levels of KLF4 and C-MYC protein at levels similar to embryonic germ cells (EGCs) but lower levels of SOX2 and OCT4. Transfection with both SOX2 and OCT4 together was required to induce PGCs to a pluripotent state at an efficiency of 1.71%, and the further addition of C-MYC increased the efficiency to 2.33%. Immunohistochemical analyses of the SOderived PGC-iPSCs revealed that these cells were more similar to ESCs than EGCs regarding both colony morphology and molecular characterization. Although leukemia inhibitory factor (LIF) was not required for the generation of PGC-iPSCs like EGCs, the presence of LIF combined with ectopic exposure to C-MYC yielded higher efficiencies. Additionally, the SO-derived PGC-iPSCs exhibited differentiation into representative cell types from all three germ layers in vitro and successfully formed teratomas in vivo. Several lines were generated that were karyotypically stable for up to 24 subcultures. Their derivation efficiency and survival in culture significantly supersedes that of EGCs, demonstrating their utility as a powerful model for studying factors regulating pluripotency in future studies.

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Section: Discussionmentioning

confidence: 99%

Direct Reprogramming of Human Primordial Germ Cells into Induced Pluripotent Stem Cells: Efficient Generation of Genetically Engineered Germ Cells

Bazley

Liu

Yuan

et al. 2015

Stem Cells and Development

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“…Teratoma-like masses containing mesoderm, ectoderm and endoderm proteins were observed 6-8 weeks after the injection of hADc-iPS into immunodeficient mice. 56 In conclusion, hADCs could be an ideal source to efficiently reprogram into individual-specific iPS cells.…”

Section: Placental Stem Cellsmentioning

confidence: 97%

Induced pluripotent stem (iPS) cells from human fetal stem cells (hFSCs)

2013

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“…It has also been reported that a specific expression profile of stem cell markers corresponds to either a completely or partially reprogrammed cell (Chan et al, 2009). Recently, iPSCs have been derived from a variety of human tissues, such as umbilical cord matrix (Cai et al, 2010), fetal and juvenile tissues (Park et al, 2008a;Li et al, 2010;Aasen et al, 2008), placental tissue (Nagata et al, 2009;Cai et al, 2010;Zhao et al, 2010), and primary human hepatocytes. However, evidence that iPSC retain epigenetic memory of their cells of origin exist for both mouse (Kim et al, 2010;Polo et al, 2010) and human (Hu et al, 2010), which can affect their differentiation potential.…”

Section: Discovery and Characterization Of Induced Pluripotent Stem Cmentioning

confidence: 99%

Cell Transplantation: A Possible Alternative to Orthotopic Liver Transplant (OLT)

Skvorak¹,

Gramignoli²,

Hansel³

et al. 2012

Liver Transplantation - Technical Issues and Complications

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Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOG

Cited by 77 publications

References 21 publications

Direct Reprogramming of Human Primordial Germ Cells into Induced Pluripotent Stem Cells: Efficient Generation of Genetically Engineered Germ Cells

Direct Reprogramming of Human Primordial Germ Cells into Induced Pluripotent Stem Cells: Efficient Generation of Genetically Engineered Germ Cells

Induced pluripotent stem (iPS) cells from human fetal stem cells (hFSCs)

Cell Transplantation: A Possible Alternative to Orthotopic Liver Transplant (OLT)

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