Rapid and Efficient Methods to Isolate, Type Strains and Determine Species of Agrobacterium spp. in Pure Culture and Complex Environments

Shams, Malek; Campillo, Tony; Lavire, Céline; Müller, Daniel; Nesme, Xavier; Vial, Ludovic

doi:10.5772/36068

Cited by 9 publications

(5 citation statements)

References 38 publications

(27 reference statements)

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“…For the total cell count, the suspension was plated on R2A agar (Sigma Aldrich, Diegem, Belgium) with cycloheximide (200 mg/l). Agrobacteria colonies were selected and identified following Shams, et al 40 . A. rhizogenes was isolated using 2E-Te containing erythritol and 320 mg/l K 2 TeO 3 with cycloheximide.…”

Section: Methodsmentioning

confidence: 99%

Mineral and organic growing media have distinct community structure, stability and functionality in soilless culture systems

Grunert

Hernandez-Sanabria

Vílchez-Vargas

et al. 2016

Sci Rep

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The choice of soilless growing medium for plant nutrition, growth and support is crucial for improving the eco-sustainability of the production in horticultural systems. As our current understanding of the functional microbial communities inhabiting this ecosystem is still limited, we examined the microbial community development of the two most important growing media (organic and mineral) used in open soilless horticultural systems. We aimed to identify factors that influence community composition over time, and to compare the distribution of individual taxa across growing media, and their potential functionality. High throughput sequencing analysis revealed a distinctive and stable microbial community in the organic growing medium. Humidity, pH, nitrate-N, ammonium-N and conductivity were uncovered as the main factors associated with the resident bacterial communities. Ammonium-N was correlated with Rhizobiaceae abundance, while potential competitive interactions among both Methylophilaceae and Actinobacteridae with Rhizobiaceae were suggested. Our results revealed that soilless growing media are unique niches for diverse bacterial communities with temporal functional stability, which may possibly impact the resistance to external forces. These differences in communities can be used to develop strategies to move towards a sustainable horticulture with increased productivity and quality.

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Section: Methodsmentioning

confidence: 99%

Mineral and organic growing media have distinct community structure, stability and functionality in soilless culture systems

Grunert

Hernandez-Sanabria

Vílchez-Vargas

et al. 2016

Sci Rep

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“…Non-pathogenic Agrobacterium strains have also been isolated from tumors (Bélanger et al, 1995; Llop et al, 2009; Shams et al, 2012). Some strains could be mutants of the initial tumor-inducing strain (e.g., following spontaneous deletions inside pTi), but most of these non-pathogenic isolates may have an environmental origin.…”

Section: Introductionmentioning

confidence: 99%

Ecological Conditions and Molecular Determinants Involved in Agrobacterium Lifestyle in Tumors

et al. 2019

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The study of pathogenic agents in their natural niches allows for a better understanding of disease persistence and dissemination. Bacteria belonging to the Agrobacterium genus are soil-borne and can colonize the rhizosphere. These bacteria are also well known as phytopathogens as they can cause tumors (crown gall disease) by transferring a DNA region (T-DNA) into a wide range of plants. Most reviews on Agrobacterium are focused on virulence determinants, T-DNA integration, bacterial and plant factors influencing the efficiency of genetic transformation. Recent research papers have focused on the plant tumor environment on the one hand, and genetic traits potentially involved in bacterium-plant interactions on the other hand. The present review gathers current knowledge about the special conditions encountered in the tumor environment along with the Agrobacterium genetic determinants putatively involved in bacterial persistence inside a tumor. By integrating recent metabolomic and transcriptomic studies, we describe how tumors develop and how Agrobacterium can maintain itself in this nutrient-rich but stressful and competitive environment.

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“…In addition to the Ti plasmid, A. tumefaciens cells often harbor a companion At plasmid which can co-transfer with the virulent plasmid (Lassalle et al, 2011;Shams et al, 2012;Lang et al, 2013). The bacterial gene blcC (=attM), located on the companion At plasmid of A. tumefaciens C58, codes for a lactonase, which both confers the ability to grow on gamma-butyrolactone as the sole source of carbon and is able to cleave the butyrolactone ring of the QS signal OC8HSL (Zhang et al, 2002;Carlier et al, 2004;Chai et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

The plant GABA signaling downregulates horizontal transfer of the Agrobacterium tumefaciens virulence plasmid

et al. 2015

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SummaryIn the tumor-inducing (Ti) Agrobacterium tumefaciens, quorum sensing activates the horizontal transfer of the virulent Ti plasmid. In pure culture, this process can be impaired by the A. tumefaciens BlcC lactonase, whose expression is induced by gamma-aminobutyrate (GABA). It was therefore hypothesized that host GABA content might modulate quorum sensing and virulence gene dissemination during A. tumefaciens infection.We examined GABA metabolism and transport in Arabidopsis thaliana tumors combining transcriptomic, metabolomic and histological approaches. In addition, using genetically modified plants and bacteria, we evaluated the impact of plant host GABA content on Ti plasmid dissemination.The results showed that GABA and free proline, which acts as an antagonist of GABA uptake in A. tumefaciens, accumulated in wild-type tumors relative to uninfected plant tissues. Moreover, comparisons of tumors induced on Col-0 and her1 plants showed that the increase in the plant GABA : proline ratio was associated with both the upregulated expression of the blcC gene and the decreased dissemination of Ti plasmid in tumor-colonizing A. tumefaciens populations.This work demonstrates experimentally that the variation in the GABA content in plant tumors can interfere with the dissemination of A. tumefaciens Ti plasmids, and therefore highlights plant GABA content as an important trait in the struggle against pathogenic bacteria.

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Rapid and Efficient Methods to Isolate, Type Strains and Determine Species of Agrobacterium spp. in Pure Culture and Complex Environments

Cited by 9 publications

References 38 publications

Mineral and organic growing media have distinct community structure, stability and functionality in soilless culture systems

Mineral and organic growing media have distinct community structure, stability and functionality in soilless culture systems

Ecological Conditions and Molecular Determinants Involved in Agrobacterium Lifestyle in Tumors

The plant GABA signaling downregulates horizontal transfer of the Agrobacterium tumefaciens virulence plasmid

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