Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs

Shan, Qiwei; Wang, Yanpeng; Chen, Kunling; Liang, Zhen; Jun, Li; Zhang, Yi; Zhang, Kang; Liu, Jin-Xing; Voytas, Daniel F.; Zheng, Xuelian; Zhang, Yong; Gao, Caixia

doi:10.1093/mp/sss162

Cited by 252 publications

(145 citation statements)

References 12 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…show that genome-editing dramatically simplifies plant breeding even in major crops with potential impact on the future of agriculture and human nutrition. However, the modification efficiency appears to vary based on the locus selected [17,19], although the selection of genome-editing systems [15] and crop species [17,23] has no significant effect on the efficiency [ Although it is difficult to identify off-target mutations in the plant genome, breeders should demonstrate that there are no significant off-target mutations that are associated with potential health or environmental risks. Otherwise, the imprudent use of genome-editing may lead to its rejection in agricultural and environmental applications.…”

Section: Genome-editing-mediated Plant Breedingmentioning

confidence: 99%

Towards social acceptance of plant breeding by genome editing

Araki

Ishii

2015

Trends in Plant Science

199

132

View full text Add to dashboard Cite

Although genome-editing technologies facilitate efficient plant breeding without introducing a transgene, it is creating indistinct boundaries in the regulation of genetically modified organisms (GMOs). Rapid advances in plant breeding by genome-editing require the establishment of a new global policy for the new biotechnology, while filling the gap between process-based and product-based GMO regulations. In this opinion article we review the recent developments for producing major crops using genome-editing and propose a regulatory model that takes into account the various methodologies to achieve genetic modifications as well as the resulting types of mutation. Moreover, we discuss the future integration of genome-edited crops into society, specifically a possible response to the Right-to-Know movement which demands labelling of food that contains genetically-engineered ingredients.The need for regulatory models Genome-editing via technologies such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)/Cas systems (e.g. Cas9) offers the ability to perform robust genetic engineering in many species [1][2][3] Genome-editing-mediated plant breedingConventional genetic engineering begins with extracellular DNA manipulation to construct a plasmid vector harboring a gene or a specific DNA sequence to be transferred into the chosen organism. The entire plasmid or only the DNA fragment is then shot into plant cells by using particle bombardment or delivered into the cells by

show abstract

Section: Genome-editing-mediated Plant Breedingmentioning

confidence: 99%

Towards social acceptance of plant breeding by genome editing

Araki

Ishii

2015

Trends in Plant Science

199

132

View full text Add to dashboard Cite

show abstract

“…Moreover, TALENs appear to be more mutagenic than ZFNs (Chen et al, 2013) and are highly specific (Juillerat et al, 2014). To promote TALEN technology, we developed a streamlined TALEN assembly method (Cermak et al, 2011), which was later used for successful genome editing in many plant species Shan et al, 2013a;Wendt et al, 2013;Zhang et al, 2013;Lor et al, 2014;Wang et al, 2014). Unfortunately, both ZFN and TALEN technologies require engineering of DNA-binding domains for individual targeting applications, demanding significant effort and expertise in molecular cloning.…”

mentioning

confidence: 99%

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

et al. 2015

Self Cite

View full text Add to dashboard Cite

The relative ease, speed, and biological scope of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated Protein9 (Cas9)-based reagents for genomic manipulations are revolutionizing virtually all areas of molecular biosciences, including functional genomics, genetics, applied biomedical research, and agricultural biotechnology. In plant systems, however, a number of hurdles currently exist that limit this technology from reaching its full potential. For example, significant plant molecular biology expertise and effort is still required to generate functional expression constructs that allow simultaneous editing, and especially transcriptional regulation, of multiple different genomic loci or multiplexing, which is a significant advantage of CRISPR/Cas9 versus other genome-editing systems. To streamline and facilitate rapid and wide-scale use of CRISPR/Cas9-based technologies for plant research, we developed and implemented a comprehensive molecular toolbox for multifaceted CRISPR/Cas9 applications in plants. This toolbox provides researchers with a protocol and reagents to quickly and efficiently assemble functional CRISPR/Cas9 transfer DNA constructs for monocots and dicots using Golden Gate and Gateway cloning methods. It comes with a full suite of capabilities, including multiplexed gene editing and transcriptional activation or repression of plant endogenous genes. We report the functionality and effectiveness of this toolbox in model plants such as tobacco (Nicotiana benthamiana), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa), demonstrating its utility for basic and applied plant research.

show abstract

“…Binding of TALEN monomers to the targeted gene allows the FokI nuclease domain to dimerize and cleave the DNA (Chen and Gao, 2013;Voytas, 2013). TALENs have been successfully used to make sequence-specific mutations in a variety of plant species, including Arabidopsis (Arabidopsis thaliana), tobacco (Nicotiana tabacum), rice (Oryza sativa), Brachypodium spp., and barley (Hordeum vulgare; Cermak et al, 2011;Li et al, 2012;Christian et al, 2013;Shan et al, 2013a;Wendt et al, 2013;Zhang et al, 2013). More recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein9 (Cas9) system has also been demonstrated to be a promising genome-editing tool (Jiang et al, 2013;Li et al, 2013;Nekrasov et al, 2013;Shan et al, 2013b;Upadhyay et al, 2013).…”

mentioning

confidence: 99%