Rapid and Efficient Ambient Temperature X-ray Crystal Structure Determination at Turkish Light Source

Abstract

High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish Light Source (Turkish DeLight). The complete dataset was collected in 18.5 mins with resolution extending to 2.39 A and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage.

The crystal structure of Vibrio cholerae (6-4) photolyase reveals interactions with cofactors and a DNA-binding region

“…N16961( Vc ) which possesses the catalytic cofactor FAD, an antenna chromophore DMRL, and an additional cofactor [4Fe-4S] cluster ( 15 ). We determined the crystal structure of Vc (6-4) PL at 2.5 Å resolution at cryogenic temperature at the Turkish Light Source known as “ Turkish DeLight ” ( 24 , 25 ). The structure contains all the cofactors with well-defined electron densities and contains a total of 24 α helixes and five β sheets ( Fig.…”

Structural Characterization of TRAF6 N-terminal for Therapeutic Uses

“…The crystal belongs to the P1 triclinic space group with a=45.885, b=51.7048, c=54.3003, α=91.04, β=112.15, γ=108.43. The dimerized structure of TRAF6 N-terminal region was determined to 2.6 Å resolution at cryogenic temperature at the Turkish Light Source “ Turkish DeLight ” [40] (Figure 1). The determined structure was deposited to PDB database with the ID: 8HZ2.…”

The crystal structure ofVibrio cholerae(6-4) photolyase reveals interactions with cofactors and a DNA binding region

“…N16961( Vc ) which possesses catalytic cofactor FAD, an antenna chromophore DMRL, and additional cofactor [4Fe-4S] cluster (15). We determined the crystal structure of Vc (6-4) PL at 2.5 Å resolution at cryogenic temperature at the Turkish Light Source known as “Turkish DeLighf (24, 25). The structure contains all the cofactors with well-defined electron densities and contains a total of 24 α helixes and 5 β sheets ( Figure 1A ).…”