Rapid and convenient separation of phospholipids and non phosphorus lipids from rat heart using silica cartridges

Juanéda, Pierre; Rocquelin, G.

doi:10.1007/bf02534360

Cited by 560 publications

(251 citation statements)

References 5 publications

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“…Polar (PL) and neutral (NL) lipids were separated according to Juaneda and Rocquelin (1985) using SepPak® silica cartridges (Waters, Milford, MA, USA).…”

Section: Animals and Dietsmentioning

confidence: 99%

The use of stoned olive cake and rolled linseed in the diet of intensively reared lambs: effect on the intramuscular fatty-acid composition

Mele

Serra

Pauselli

et al. 2014

Animal

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The aim of the present study was to evaluate the effect of the inclusion of stoned olive cake and rolled linseed in a concentratebased diet for lambs on the fatty-acid composition of polar and non-polar intramuscular lipids of the longissimus dorsi muscle. To achieve this objective, 32 Appenninica lambs were randomly distributed into four groups of eight lambs each and were fed conventional cereal-based concentrates (diet C); concentrates containing 20% on a dry matter (DM) basis of rolled linseed (diet L); concentrates containing 35% DM of stoned olive cake (diet OC); and concentrates containing both rolled linseed (10% DM) and stoned olive cake (17% DM; diet OCL). The concentrates were administered together with grass hay at a 20 : 80 forage : concentrate ratio. Growing performances and carcass traits were evaluated. The fatty-acid composition was analysed in the total intramuscular lipids, as well as in the polar and neutral lipids. The average feed intake and the growth performance of lambs were not affected by the dietary treatments, as a consequence of similar nutritional characteristics of the diets. The inclusion of rolled linseed in the L and OCL diets increased the content of C18:3 n-3 in intramuscular total lipids, which was threefold higher in meat from the L lambs and more than twofold higher in meat from the OCL lambs compared with the C and OC treatments. The n-6 : n-3 ratio significantly decreased in the meat from lambs in the L and OCL groups, reaching values below 3. The L treatment resulted in the highest level of trans-18:1 fatty acids in the muscle. Regardless of the dietary treatment, the t10-18:1 was the major isomer, representing 55%, 45%, 49% and 45% of total trans-18:1 for C, L, OC and OCL treatments, respectively. Neutral lipids from the OC-fed lambs contained the highest amount of c9-18:1 (more than 36% of total fatty acids); however, the content of c9-18:1 did not differ between the OC and C lambs, suggesting an intensive biohydrogenation of dietary c9-18:1 in the case of OC treatment. The highest content of c9,t11-18:2 was detected in the intramuscular fat from the L-fed lambs, followed by the OCL treatment. A similar trend was observed in the neutral lipid fraction and, to a lower extent, in the polar lipids.

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“…Polar (PL) and neutral (NL) lipids were separated according to Juaneda and Rocquelin (1985) using SepPak® silica cartridges (Waters, Milford, MA, USA).…”

Section: Animals and Dietsmentioning

confidence: 99%

The use of stoned olive cake and rolled linseed in the diet of intensively reared lambs: effect on the intramuscular fatty-acid composition

Mele

Serra

Pauselli

et al. 2014

Animal

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“…For quantitative analysis, a silica cartridge (25 mm x 10 mm) (SEP-Pack waters, Framingham, USA) was used to separate non-phosphorus lipids from phospholipids with 30 ml of chloroform followed by 5 ml of chloroform/methanol (Juaneda and Rocquelin, 1985). Phospholipid molecular classes were separated by high-performance liquid chromatography (HPLC) using a Beckman apparatus.…”

mentioning

confidence: 99%

Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids

Frémont¹,

Riazi²

1988

Reprod. Nutr. Dévelop.

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Summary. Vitellogenin was obtained from three year-old vitellogenic trout.Two procedures of isolation were compared : dialysis against distilled water and ultracentrifugation in the density interval 1 .21 -1 .28 g/ml.Similar patterns were observed by gel filtration and electrophoresis for both preparations of vitellogenin, indicating that electric charge and molecular weight were not modified by either procedure.The apparent M, of the native form was 560,000 in gel filtration, whereas that of the monomer was estimated as 170,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Minor proteins were also detected (100,000-88,000-78,000).The main components were protein (79%), and lipids (19 %), Carbohydrates accounted for 0.3 % when protein phosphorus and calcium each represented 0.7 % of total weight.Phospholipids (70 % of total lipids) mainly consisted of phosphatidylcholine in which n-3 docosahexanenoic acid accounted for one-third of total fatty acids.The results show the high levels of essential fatty acids in structural lipids which are known to be involved in embryo development.Introduction.

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“…Lipids from fish muscle/liver were separated into neutral lipid and polar lipid according to the procedures described by Juaneda and Rocquelin (1985). The total lipid extracts (100 − 200 mg) were fractionated on silica cartridges (Sep-Pak ® Vac 6cc, Waters); neutral lipid was eluted with chloroform and polar lipid with methanol.…”

Section: Lipid Extract and Fatty Acid Analysis Of Fishmentioning

confidence: 99%

Lipid and fatty acid compositions of cod (Gadus morhua), haddock (Melanogrammus aeglefinus) and halibut (Hippoglossus hippoglossus)

Zeng

Mai

et al. 2010

J. Ocean Univ. China

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Rapid and convenient separation of phospholipids and non phosphorus lipids from rat heart using silica cartridges

Cited by 560 publications

References 5 publications

The use of stoned olive cake and rolled linseed in the diet of intensively reared lambs: effect on the intramuscular fatty-acid composition

The use of stoned olive cake and rolled linseed in the diet of intensively reared lambs: effect on the intramuscular fatty-acid composition

Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids

Lipid and fatty acid compositions of cod (Gadus morhua), haddock (Melanogrammus aeglefinus) and halibut (Hippoglossus hippoglossus)

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