Rapid and cheap prototyping of a microfluidic cell sorter

Islam, Md. Zahurul; McMullin, J.N.; Tsui, Ying Y.

doi:10.1002/cyto.a.21063

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…As an example, we have simulated the flows in the microchannels of a microfluidic cytometer chip using the Q3D model. One potential application of such a microfluidic cytometer is the sorting of cells from blood [33,34]. The schematic diagram of the cytometer chip is shown in Figure 9.…”

Section: Comparison Of Experimental and Simulation Resultsmentioning

confidence: 99%

Quasi-3D Modeling and Efficient Simulation of Laminar Flows in Microfluidic Devices

Islam

Tsui

2016

Sensors

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A quasi-3D model has been developed to simulate the flow in planar microfluidic systems with low Reynolds numbers. The model was developed by decomposing the flow profile along the height of a microfluidic system into a Fourier series. It was validated against the analytical solution for flow in a straight rectangular channel and the full 3D numerical COMSOL Navier-Stokes solver for flow in a T-channel. Comparable accuracy to the full 3D numerical solution was achieved by using only three Fourier terms with a significant decrease in computation time. The quasi-3D model was used to model flows in a micro-flow cytometer chip on a desktop computer and good agreement between the simulation and the experimental results was found.

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Section: Comparison Of Experimental and Simulation Resultsmentioning

confidence: 99%

Quasi-3D Modeling and Efficient Simulation of Laminar Flows in Microfluidic Devices

Islam

Tsui

2016

Sensors

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“…Inexpensive fluidic switches can also be realized using solenoid valves. 22 However, compared to these, our approach has two main advantages: First of all, the Braille valves are aligned with the channels and thus produce minimal or no dead volumes. In contrast, external solenoid valves typically have dead volumes of several microliters which are not tolerable when working with precious samples or limited material such as primary cells.…”

Section: ■ Conclusionmentioning

confidence: 99%

A Versatile, Low-Cost, Multiway Microfluidic Sorter for Droplets, Cells, and Embryos

et al. 2018

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Partitioning and sorting particles, including molecules, cells and organisms, is an essential prerequisite for a diverse range of applications. Here, we describe a very economical microfluidic platform (built from parts costing about U.S. $6800 for a stand-alone system or U.S. $3700, when mounted on an existing fluorescence microscope connected to a computer) to sort droplets, cells and embryos, based on imaging data. Valves operated by a Braille display are used to open and close microfluidic channels, enabling sorting at rates of >2 Hz. Furthermore, we show microfluidic 8-way sorting for the first time, facilitating the simultaneous separation and collection of objects with diverse characteristics/phenotypes. Due to the high flexibility in the size of objects that can be sorted, the low cost, and the many possibilities enabled by imaging technology, we believe that our approach nicely complements existing FACS and μFACS technology.

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“…However, it needs large investment, is relatively slow when high numbers of cells with a high purity are needed and aerosol formation by the droplet sorting may render a risk [11]. Microfluidic cell sorters avoid aerosol borne risk but are mostly slower than FACS and allow sorting of one cell population only [12]. …”

Section: Introductionmentioning

confidence: 99%

An Innovative Cascade System for Simultaneous Separation of Multiple Cell Types

et al. 2013

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Isolation of different cell types from one sample by fluorescence activated cell sorting is standard but expensive and time consuming. Magnetic separation is more cost effective and faster by but requires substantial effort. An innovative pluriBead-cascade cell isolation system (pluriSelect GmbH, Leipzig, Germany) simultaneously separates two or more different cell types. It is based on antibody-mediated binding of cells to beads of different size and their isolation with sieves of different mesh-size. For the first time, we validated the pluriSelect system for simultaneous separation of CD4+- and CD8+-cells from human EDTA-blood samples. Results were compared with those obtained by magnetic activated cell sorting (MACS; two steps -first isolation of CD4+, then restaining of the residual cell suspension with anti-human CD8+ MACS antibody followed by the second isolation). pluriSelect separation was done in whole blood, MACS separation on density gradient isolated mononuclear cells. Isolated and residual cells were immunophenotyped by 7-color 9-marker panel (CD3; CD16/56; CD4; CD8; CD14; CD19; CD45; HLA-DR) using flow cytometry. Cell count, purity, yield and viability (7-AAD exclusion) were determined. There were no significant differences between both systems regarding purity (MACS (median[range]: 92.4% [91.5-94.9] vs. pluriSelect 95% [94.9-96.8])) of CD4+ cells, however CD8+ isolation showed lower purity by MACS (74.8% [67.6-77.9], pluriSelect 89.9% [89.0-95.7]). Yield was not significantly different for CD4 (MACS 58.5% [54.1-67.5], pluriSelect 67.9% [56.8-69.8]) and for CD8 (MACS 57.2% [41.3-72.0], pluriSelect 67.2% [60.0-78.5]). Viability was slightly higher with MACS for CD4+ (98.4% [97.8-99.0], pluriSelect 94.1% [92.1-95.2]) and for CD8+-cells (98.8% [98.3-99.1], pluriSelect 86.7% [84.2-89.9]). pluriSelect separation was substantially faster than MACS (1h vs. 2.5h) and no pre-enrichment steps were necessary. In conclusion, pluriSelect is a fast, simple and gentle system for efficient simultaneous separation of two and more cell subpopulation directly from whole blood and provides a simple alternative to magnetic separation.

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Rapid and cheap prototyping of a microfluidic cell sorter

Cited by 8 publications

References 21 publications

Quasi-3D Modeling and Efficient Simulation of Laminar Flows in Microfluidic Devices

Quasi-3D Modeling and Efficient Simulation of Laminar Flows in Microfluidic Devices

A Versatile, Low-Cost, Multiway Microfluidic Sorter for Droplets, Cells, and Embryos

An Innovative Cascade System for Simultaneous Separation of Multiple Cell Types

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