Rapid and automated detection of fluorescent total bacteria in water samples

Lepeuple, A; Gilouppe, S.; Pierlot, E; Roubín, Mirian Raposeiras

doi:10.1016/j.ijfoodmicro.2003.08.013

Cited by 15 publications

(15 citation statements)

References 9 publications

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“…Normally, VBNC bacteria possess negligible metabolic activity and can be resuscitated in the proper conditions; therefore, they are able to be cultivated again [38,39]. In this case, evaluating bacterial inactivation using the colony-counting methodology would be inaccurate [40,41]. However, critical point in the VBNC definition is the resuscitation.…”

Section: Introductionmentioning

confidence: 99%

Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Doležalová

Lukeš

2015

Bioelectrochemistry

148

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Section: Introductionmentioning

confidence: 99%

Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Doležalová

Lukeš

2015

Bioelectrochemistry

148

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“…2NBDG can be taken only by the cells with high viability and concentrated in the cells [29]. This contrasts other dyes that enter the cells by passive diVusion [11,13,22]. In comparison with DNA staining dyes, 2NBDG is thought to be more speciWc to viable cells and therefore less liable to cause pseudo positive data.…”

Section: Discussionmentioning

confidence: 99%

Rapid evaluation of the efficacy of microbial cell removal from fabrics

Fujioka¹,

Kozone

Saito

et al. 2006

J Ind Microbiol Biotechnol

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The efficacy of microbial cell removal (EMR) from fabrics is a practically important indicator for the evaluation of cleansers and detergents. EMR is expressed quantitatively by the relative number of viable cells remaining on a fabric swatch after the treatment with these reagents. In order to count the viable cells on the swatch directly and rapidly, we have developed a unique microscopic imaging system with an ultra-deep focusing range. Standard swatches of cotton fabric were inoculated with microorganisms such as Pseudomonas fluorescence, Staphylococcus aureus, or Candida albicans. After the incubation on an agar medium, each swatch was treated with a fluorescent glucose, 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxyglucose, to stain only viable cells. The images of every cell distributed within the surface layer with no greater than 130 microm thickness could be integrated into one image. Thus visualized cells could be counted automatically by a novel imaging program. Using a pair of cotton swatches (0.5 x 1.0 cm(2)) inoculated with C. albicans, EMR was evaluated quantitatively. Before washing, the total number of viable cells found on the observation area (3.8 x 10(-4 )cm(2)) was 288 cells. After washing with a test detergent, no cell (<1) was detected. For this case, EMR was given by the formula: log(288/<1)=greater than 2.5. The imaging and cell count of a test fabric could be performed within 1 h.

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“…Le développement des techniques analytiques de biologie moléculaire et cellulaire a rendu possible l'étude directe des espèces microbiennes au niveau cellulaire sans étape de culture (AMANN et al, 1995;JOUX et LEBARON, 2000). Le développement d'instruments de mesure, tels que la cytométrie en phase solide ou en flux, a également contribué à l'utilisation de ces méthodes directes en microbiologie industrielle (ALLEGRA et al, 2008;LEPEUPLE et al, 2004;PHE et al, 2005). En comparant les données obtenues avec ces méthodes directes et les techniques de cultures traditionnelles, il a été montré que seulement à 0,001 à 15 % de la flore microbienne totale est cultivable dans un environnent donné (AMANN et al, 1995).…”

Section: La Découverte De La Partie Cachée De L'iceberg : Les Microorunclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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L’émergence de pathogènes dans l’eau destinée à la consommation humaine représente une préoccupation majeure en matière de santé publique pour les industriels et les pouvoirs publics concernés. Parmi ces pathogènes, certains sont d’origine fécale (Cryptosporidium, Campylobacter ou bien les rotavirus), alors que d’autres vivent dans l’environnent naturel (Legionella, Pseudomonas, Aeromonas ou bien les mycobactéries). Dans l’optique de mettre en place une analyse des risques liés à la présence de ces pathogènes, il est important d’accroître nos connaissances sur l’écologie de ces microorganismes et de développer des outils d’analyse afin de réaliser une meilleure surveillance sanitaire. Par conséquent, l’écologie microbienne du réseau de distribution d’eau potable doit être étudiée en détail, particulièrement vis-à-vis des propriétés physiologiques et la diversité des espèces microbiennes présentes, afin de mieux comprendre les interactions entre les espèces communément rencontrées et celles pathogènes.The emergence of pathogens in water intended for human consumption is a major concern in terms of the public health industry and the public authorities concerned. Among these pathogens, some are of faecal origin (Cryptosporidium, Campylobacter, or rotavirus), while others live in the natural environment (Legionella, Pseudomonas, Aeromonas or mycobacteria). In order to establish a risk analysis related to the presence of these pathogens, it is important to increase our knowledge on the ecology of these microorganisms and to develop analytical tools to achieve better health monitoring. Therefore, the microbial ecology of drinking water distribution networks must be studied in detail, especially with respect to the properties and physiological diversity of the microbial species present, in order to better understand the interactions between species commonly encountered and those that are pathogens

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Rapid and automated detection of fluorescent total bacteria in water samples

Cited by 15 publications

References 9 publications

Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Rapid evaluation of the efficacy of microbial cell removal from fabrics

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

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