Rapid and accurate viral diagnosis

Landry, Marie L.; Mayo, Donald R.; Hsiung, G. D.

doi:10.1016/0163-7258(89)90098-3

Cited by 5 publications

(2 citation statements)

References 325 publications

(251 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16 The clinical significance of positive results for such a highly sensitive method remains unclear. 17 Queipo-Ortuno et al 4 did not find a single positive case in their control group, perhaps due to the difference in exposure and protective measures in the two groups. The intermittent exposure of these people might have led to a localized infection or represent a very early stage of the disease not sufficient to produce detectable antibodies, 16 however, the possibility of inadequately treated or self-limited past infection 18 cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of PCR, Culture and Serology for the Diagnosis of Acute Human Brucellosis

et al. 2000

View full text Add to dashboard Cite

Background:The diagnosis of brucellosis is frequently difficult to establish. This is not only because clinically, the disease can mimic any infectious and noninfectious disease, but also because the established diagnostic methods are not always successful. In this study, we have tried to evaluate PCR techniques in the diagnosis of brucellosis in comparison to conventional techniques. Patients and Methods: Fifty peripheral blood samples from the following groups were collected: patients with brucellosis (17); patients with febrile illnesses due to factors other than brucella etiology (19); symptomatic occupationally exposed persons (9); and healthy volunteers (5). The last three groups were considered controls. Among the 17 Brucella samples, only 14 were obtained before treatment was begun. The samples were tested by serology, using the standard tube agglutination method (STA), blood culture using Bactec machines, and PCR using primer pair to amplify a 223-bp region within a gene coding for a 31-kD Brucella antigen. Diagnosis of brucellosis was based on compatible clinical picture in addition to positive blood culture and/or positive serology. Results: Of the 17 blood samples from patients with brucellosis, eight were culture positive for Brucella species, and all showed high titer antibrucella antibodies. Only 14 of them were positive by PCR, and these were the samples submitted before initiation of therapy, representing 100% sensitivity. Among the 33 controls, blood culture was negative for Brucella in all of them, while one sample showed high-titer antibrucella antibodies. The latter was from the febrile illnesses group. PCR-based assay was able to detect four bands in the controls, all of which were from the occupationally exposed asymptomatic group. Conclusion: In view of the several advantages of PCR over the conventional methods for the diagnosis of brucellosis, such as speed, safety, high sensitivity and specificity, the technique might be considered for laboratory diagnosis of brucellosis. However, for the evaluation of asymptomatic highly exposed persons, PCR might be considered complementary to the traditional methods and followed up by serology and/or culture.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of PCR, Culture and Serology for the Diagnosis of Acute Human Brucellosis

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Through the pioneering efforts of clinical virologists such as G. D. Hsiung, a large body of information about the suit-ability of particular cell lines for detecting and propagating particular human pathogenic viruses has been accumulated over the past three to four decades. In addition, Hsiung has championed an ongoing search for more sensitive cell culture systems for detecting pathogenic viruses including those currently not cultivable (38,39,43).…”

Section: History Of Virus Culturementioning

confidence: 99%

Transgenic cell lines for detection of animal viruses

Olivo

1996

Clin Microbiol Rev

View full text Add to dashboard Cite

Rapid diagnostic assays based on direct detection of viral antigen or nucleic acid are being used with increasing frequency in clinical virology laboratories. Virus culture, however, remains the only way to detect infectious virus and to analyze clinically relevant viral phenotypes, such as drug resistance. Growth of viruses in cell culture is labor intensive and time-consuming and requires the use of many different cell lines. Transgenic technology, together with increasing knowledge of the molecular pathways of virus replication, offers the possibility of using genetically modified cell lines to improve virus growth in cell culture and to facilitate detection of virus-infected cells. Genetically modifying cells so that they express a reporter gene only after infection with a specific virus can allow the detection of infectious virus by rapid and simple enzyme assays such as beta-galactosidase assays without the need for antibodies. Although transgenic cells have recently been successfully used for herpes simplex virus detection, much more work needs to be done to adapt this technology to other human viral pathogens such as cytomegalovirus and respiratory viruses. This review offers some strategies for applying this technology to a wide spectrum of animal viruses.

show abstract

Genetically Engineered Cells in Diagnostic Virology

Olivo

Infectious Agents and Pathogenesis

View full text Add to dashboard Cite

Rapid and accurate viral diagnosis

Cited by 5 publications

References 325 publications

Evaluation of PCR, Culture and Serology for the Diagnosis of Acute Human Brucellosis

Evaluation of PCR, Culture and Serology for the Diagnosis of Acute Human Brucellosis

Transgenic cell lines for detection of animal viruses

Genetically Engineered Cells in Diagnostic Virology

Contact Info

Product

Resources

About