Background and ObjectivesMeticulous dissection and identification of nerves during head and neck surgery are crucial for preventing nerve damage. At present, nerve identification relies heavily on the surgeon's knowledge of anatomy, optionally combined with intraoperative neuromonitoring. Recently, optical techniques such as Mueller polarimetric imaging (MPI) have shown potential to improve nerve identification.Study Design/Materials and MethodsWith institutional approval, seven 25–35 kg Yorkshire pigs underwent cervical incision in the central neck. Intraoperative images were obtained using our in‐house MPI system. Birefringence maps from the MPI system were processed to quantify the values between 0 and 255 from different tissue types; an active contour model was applied to further improve nerve visualization on the corresponding color images.ResultsAmong the seven pigs, the vagus nerves and recurrent laryngeal nerves were successfully differentiated with a mean intensity of 130.954 ± 20.611, which was significantly different (P < 0.05) from those of arteries (78.512 ± 27.78) and other surrounding tissues (82.583 ± 35.547). There were no imaging‐related complications during the procedure. © 2021 Wiley Periodicals LLC.ConclusionsMPI is a potentially complementary intraoperative tool for nerve identification in adjacent tissues.