Rapid and accurate amperometric determination of acetaminophen in pharmaceutical preparations and spiked human blood serum samples at cadmium pentacyanonitrosylferrate modified glassy carbon electrode

Abstract

The present work describes a rapid and accurate amperometric technique for the determination of acetaminophen (ACT) in pharmaceutical preparations and human blood serum, based on electrocatalytic oxidation of ACT at a glassy carbon electrode modified by cadmium pentacyanonitrosylferrate (CdPCNF) film. The electrocatalytic response of the modified GC electrode was linear over the concentration of 1.64-52.90 μM. The limit of detection was found to be 2.04 μM by amperometric technique. The method was successfully utilized for the determination of ACT in various pharmaceutical preparations and the results have been statistically compared with those obtained by the official method. The interference of some pharmaceutical and biological compounds was investigated. The results of interference study showed that the Nafion-coated CdPCNF|GC electrode can be utilized as a selective amperometric sensor for acetaminophen determination in human blood serum. The mean value of rate constant k for catalytic reaction, and the diffusion coefficient of ACT (D) in the phosphate buffer solution of pH 7.2 were found to be 4.27 10 2 M -1 s -1 , and (4.25 ± 0.33) 10 -6 cm 2 s -1 , respectively.

Electrochemical behavior of a glassy-carbon electrode chemically modified with cadmium pentacyanonitrosylferrate in the presence of tetrahydrothiophene

“…Fig. 1 shows that, during the formation process of the film, there is an increase in the anodic and cathodic currents in E pa = 580 mV and E pc = 563 mV, which is characteristic of the formation of CdPCNF [26,28]. The Cd 2+ cations cannot undergo reduction during the procedure, so the used potential range was À0.…”

“…The preparation of the CdPCNF/GC-modified electrode was performed using cyclic voltammetry (CV) [26,28]. GC was immersed in a solution containing 1 Â 10 À3 mol dm À3 CdCl 2 , 1 Â 10 À2 mol dm À3 PCNF and 0.5 mol dm À3 KNO 3 with the pH adjusted to be in the range of 2-3.…”

Electrochemical Behavior of a Glassy Carbon Electrode Chemically Modified with Nickel Pentacyanonitrosylferrate in Presence of Sulfur Compounds

“…According to the literature, the PCNF complexed to metals (MPCNF) has been used for electrocatalytic oxidation of various compounds such as: sulfite [1], thiosulfate [12], hydrazine [13], ascorbic acid [14], dopamine [15], iodine [16] and nitrite [17]. However, studies of CMEs with MPCNF have been conducted using carbon composites electrodes (CCE) [1,18,19] and films on various substrates: glassy carbon (GC) [11][12][13][14]20], aluminum [3,16,21], mercury [22] and platinum [23], while the latter two substrates are not used very often.…”

“…However, studies of CMEs with MPCNF have been conducted using carbon composites electrodes (CCE) [1,18,19] and films on various substrates: glassy carbon (GC) [11][12][13][14]20], aluminum [3,16,21], mercury [22] and platinum [23], while the latter two substrates are not used very often.…”

Graphite oxide film-modified electrode as an electrochemical sensor for acetaminophen

“…Acetaminophen contains phenolic hydroxyl group, which is electrochemical active and can be oxidized. Thus, various materials including carbon nanotube [8][9][10][11][12], carbon nanoparticles [13], poly (3,4-ethylenedioxythiophene) [14], thionine immobilized multi-walled carbon nanotube [15], gold nanoparticle [16], nano-TiO 2 /polymer [17], boron-doped diamond [18], cadmium pentacyanonitrosylferrate [19], carbon-coated nickel nanoparticles [20] and poly(taurine)/carbon nanotube [21], were used to prepare electrochemical sensors for the detection of acetaminophen. However, electrochemical detection of acetaminophen using GO has not been reported.…”