Rapid analytical procedure for neomycin determination in ointments by CE with direct UV detection

Huidobro, A.L.; Garcı́a, Antonia; Barbas, Coral

doi:10.1016/j.jpba.2009.03.005

Cited by 31 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Concretely neomycin is an aminoglycoside antibiotic naturally produced by actinomycete bacterium via fermentation process 79. Although many studies were reported in previous years describing new methodologies for the determination of aminoglycosides by CE, in the period of time covered in this review only one article by Huidobro et al 71 was published (Table 5). In this study, neomycin was determined in a pharmaceutical formulation by CZE‐UV.…”

Section: Aminoglycosidesmentioning

confidence: 99%

Recent advances in the analysis of antibiotics by CE and CEC

et al. 2011

View full text Add to dashboard Cite

This article reviews the recent developments achieved in the analysis of antibiotics by CE and CEC. CZE has mainly been employed although other separation modes have also been used. Different detection systems coupled to CE have been reported for antibiotic determination. Enhancement on sensitivity through the use of diverse offline, inline, and online preconcentration approaches is also described. Special attention has been paid to sample treatment steps and to the applications developed in different research areas such as the pharmaceutical, biological, food, and environmental. This manuscript covers the period of time from the previous review published in June 2009 until May 2011.

show abstract

Section: Aminoglycosidesmentioning

confidence: 99%

Recent advances in the analysis of antibiotics by CE and CEC

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Given the ionic character of the aminoglycosides over a wide pH range, CE can also be applied nm [75]. Netilmicin, tobramycin, lincomycin, kanamycin, and amikacin were determined simultaneously in pharmaceutical preparations using of 125 mM NaOH as a background electrolyte, an uncoated fused-silica capillary, and electrochemical detection involving a copper microparticle-modified carbon fiber microdisk array electrode.…”

Section: Capillary Electrophoresismentioning

confidence: 99%

Challenges in the determination of aminoglycoside antibiotics, a review

Farouk

Azzazy

Niessen

2015

Analytica Chimica Acta

120

View full text Add to dashboard Cite

“…A lot of researchers had employed various assay methods for determining some aminoglycosides including gentamicin in different delivery systems. These include: microbiological [5], polarographic [6], chemiluminescence [7], capillary electrophoresis [8], immunoassay [9], thin layer chromatographic [10], high performance liquid chromatographic [11], spectrofluorimetric [12], and spectrophotometric [13,14] methods.…”

Section: Introductionmentioning

confidence: 99%

Oral Gentamicin Preparation Using Solidified Lipid Particulate Delivery System

Adedokun

Jackson

Uchegbu

et al. 2017

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: Despite the broad pharmacological activity of gentamicin against a number of bacteria, it's very inadequate oral bioavailability due to poor intestinal membrane permeability has limited its formulation into oral dosage delivery system. This work was thus aimed at formulation and evaluation of gentamicin-loaded microemulsions based on preparation of lipid matrix for sustained release delivery. Methods:Oral gentamicin suspensions were prepared by emulsification method using Tween 80 as a mobile surfactant in the lipid matrix dispersion. The resultant oral suspensions were evaluated for mean particle size and morphology using a photomicrograph, encapsulation efficiency/entrapment, EE (%), dispersibility, pH and absolute drug content. Release study as a function of inhibition zone diameter (IZD) and in vitro release study was also carried out. The in vitro release study was performed in both simulated gastric fluid (pH 1.2) and simulated intestinal fluid (pH 7.2) respectively. The release data were analyzed mathematically according to zero order, first order and Higuchi equations. Results:The prepared suspensions were cream-white in colour, easily dispersed and well homogenized. Batch D, which had least amount of excipients incorporated into the lipid matrix showed clumped irregular-shaped and less free-flowing particles. The particle size was significantly influenced by lipid matrix combination ratio in the presence of a surfactant (p<0.05). The mean particle size diameters of the samples were 15.44 mm, 10.64 mm, 4.12 mm, and 2.70 mm for batches A, B, C and D respectively. The values of EE obtained varied between 47% and 59% with Batch B exhibiting the highest value. The Higuchi model gave the best release kinetics result followed by zero order kinetics. Conclusion:Oral gentamicin prepared exhibited antibacterial properties against Klebsiella spp., Escherchia coli, Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. The results suggest that a lipid matrix system could be useful as a sustained release oral delivery system of a poorly absorbable drug such as gentamicin.

show abstract

Rapid analytical procedure for neomycin determination in ointments by CE with direct UV detection

Cited by 31 publications

References 26 publications

Recent advances in the analysis of antibiotics by CE and CEC

Recent advances in the analysis of antibiotics by CE and CEC

Challenges in the determination of aminoglycoside antibiotics, a review

Oral Gentamicin Preparation Using Solidified Lipid Particulate Delivery System

Contact Info

Product

Resources

About