Rapid analysis of ionic and nonionic surfactant homologs by high performance liquid chromatography

Abstract

Several ionic and nonionic surfactants were separated into their homologs and their homologous distributions were determined by high performance liquid chromatography on an octadecyl-silica (TSK Gel LS-410) column with a water-methanol-sodium chloride mixture as a mobile phase. The recommended condition for the separation was as follows: column size, 4 mmid x 250 mm; mobile phase, water/methanol (15/85, v/v) containing 0.4 M/L sodium chloride; column temp., 50 C. Under this condition, three nonionic, four anionic and one cationic surfactants were efficiendy separated into their respective homologs. The addition of sodium chloride affected particularly the separation of strongly ionogenic surfactants such as sodium alkylsulfates and alkylbenzyldimethylammonium chlorides. Analytical results of homologous distributions of several commercial surfactants were in good accordance with those obtained by the conventional gas chromatography (GC) method. / JAOCSJanuary 1981 (SD&C 2) (SD&C 3) JAOCS January 1981 / 73

Analysis of ionogenic surfactants by HPLC with ion‐pair extraction detector

“…Therefore, for quantitative analysis, it was necessary to use the calibration curves of n-dodecyl, n-tetradecyl, n-hexadecyl and n-octadecyl derivatives. The results were in good agreement with those obtained by conventional GC methods (5).…”

“…Nakae and Tsuji (3) and Kawase et al (4) reported the application of this detector to reversed-phase HPLC for the homolog separation of anionic and cationic surfactants. However, there are few reports on the We have been studying the separation of surfactant homologs by HPLC using a reversed-phase packing containing an octadecyl silane group chemically bonded to silica gel (ODS/Silica) (5)(6)(7). In our previous paper (6), nonionic, anionic, cationic and amphoteric surfactants were separated into their individual homologs and simultaneously distinguished from each other using a mixture of methanol, water, sodium perchlorate (NaC104) and phosphoric acid as the mobile phase.…”

Determination of Benzalkonium Chloride from Biocide Products: Selectivity Study

“…Benzalkonium chloride (BAC) is a compound of the class of quaternary ammonium compounds (QAC) used as a cationic surfactant in personal hygiene, cosmetic and skin disinfection products [1]. BAC is a mixture of alkylbenzyldimethylammonium chlorides [C6H5CH2N(CH3)2RCl], in which R = C12, C14, C16, C18 are the main compounds, and the antibacterial effects being given by the alkyl chain length and the quaternary ammonium groups [1,2].In this study we compare two methods of analysis for quantitative determination: chemical titration [3] and liquid chromatography (HPLC-UV) [4]. Chemical titration is an iodometric method in a strongly acidic medium, in which the 0.05M potassium iodate solution is used as titrant (titration with extracted end point) [3].…”

“…In this study we compare two methods of analysis for quantitative determination: chemical titration [3] and liquid chromatography (HPLC-UV) [4]. Chemical titration is an iodometric method in a strongly acidic medium, in which the 0.05M potassium iodate solution is used as titrant (titration with extracted end point) [3].…”

“…HPLC determinations were performed using an Agilent 1100 Series HPLC, equipped with a binary pump, a degasser, an autosampler and a spectrophotometric detector in UV-Vis, and Agilent Chemstation software was used for data acquisition and analysis. Chromatographic parameters were injection volume, 10 μL; analysis time, 25 min; a Zorbax Eclipse XDB-C18 column (5 μm, 4.6 mm × 150 mm); and flow rate, 1.5 mL/min, isocratic conditions 20:80 A:B (v/v), where A = 0.4 M/L NaCl solution and B = methanol [4]. BAC monitoring was performed at a wavelength of 210 nm with the help of a UV-Vis spectrophotometric detector.…”