Rapid Analysis of Hemoglobins in Whole Blood by a Light Scattering Method

Lisenko, S. A.; Kugeiko, M. M.

doi:10.1007/s10812-013-9783-7

J Appl Spectrosc

2013

DOI: 10.1007/s10812-013-9783-7

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Rapid Analysis of Hemoglobins in Whole Blood by a Light Scattering Method

S. A. Lisenko

M. M. Kugeiko

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Cited by 3 publications

(8 citation statements)

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“…The accuracy of this calculation is comparable with the accuracy of the Monte Carlo method, but the calculation times for the methods differ by several orders of magnitude (on personal computers with average technical specifi cations by today's standards, the calculation of the radiation density distribution over the depth of the skin by the method in [23] takes milliseconds). Knowing the total bilirubin concentration in tissues of the dermis, the radiation density distribution over the depth of the dermis, and the illumination of the skin of the patient E(0, λ), using formula (3) we can calculate the rate of transformation of ZZ-bilirubin to the nontoxic and water-soluble isomer LR that is provided by the specifi c phototherapy system.…”

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confidence: 98%

“…The possibility for noninvasive determination of the radiation density in the tissue E(z, λ) was demonstrated for the fi rst time in [17,23], where it was proposed to use measurements of the spectral diffuse refl ection coeffi cient for the tissue, R(λ), representing the ratio of the diffuse radiation fl ux refl ected by the medium to the directional radiation fl ux incident on it. The basis for this is the dependence of the penetration depth of light into the tissue on λ.…”

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confidence: 99%

“…The probing radiation with different λ values penetrates into different layers of the tissue, and therefore contains information about these layers. Speed and accuracy of recovery of E(z, λ) from the R(λ) spectrum is achieved as a result of using a semi-analytical method for solving the radiation transport equation in an optically dense multilayer tissue [23], allowing us to calculate in a fraction of a second E(z, λ) and R(λ) in the spectral regions of strong (λ < 0.6 μm) and weak (λ = 0.6-1.0 μm) absorption of light by the tissue.…”

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confidence: 99%

“…In order to rapidly calculate the function E(z, λ), we can use the semi-analytical method in [23]. The accuracy of this calculation is comparable with the accuracy of the Monte Carlo method, but the calculation times for the methods differ by several orders of magnitude (on personal computers with average technical specifi cations by today's standards, the calculation of the radiation density distribution over the depth of the skin by the method in [23] takes milliseconds).…”

mentioning

confidence: 99%

“…1a. The radiation density distributions over depth in the skin and the wavelength of the light were calculated according to the values of x * found by the method in [23]. Illumination of the skin was assumed to be independent of λ and equal to 1 mW/cm 2 .…”

mentioning

confidence: 99%

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Method for Estimating Bilirubin Isomerization Efficiency in Phototherapy to Treat Neonatal Jaundice

Lisenko

Kugeiko

2014

J Appl Spectrosc

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We propose a method for quantitative assessment of the effi cacy of phototherapy to treat neonatal jaundice using the diffuse refl ectance spectrum for the newborn's skin, based on the analytical dependence of the measured spectrum on the structural and morphological parameters of the skin, affecting the optical conditions in the medium, and an algorithm for rapid calculation of the bilirubin photoisomerization rate in the skin tissues as a function of the structural and morphological parameters of the skin and the wavelength of the exciting radiation. From the results of a numerical simulation of the process of radiation transport in the skin, we assess the stability of our method to variations in the scattering properties of the skin and the concentrations of its optically active chromophores (melanin, oxyhemoglobin, deoxyhemoglobin). We show that in order to achieve the maximum effi cacy of phototherapy, we should use light from the range 484-496 nm. In this case, the intensity of the exciting radiation should be selected individually for each newborn according to the bilirubin photoisomerization rate characteristic for it.Introduction. Hyperbilirubinemia (jaundice) develops in newborns as a result of accumulation of excess ZZbilirubin molecules, a toxic pigment formed from hemoglobin as a result of breakdown of erythrocytes, in the blood (and consequently in the skin tissues). The most widely used method for treating hyperbilirubinemia is phototherapy. When the skin of a newborn is exposed to optical radiation, isomerization of the ZZ-bilirubin molecules found in the skin occurs, with formation of low-toxicity photoisomers: ZE-bilirubin, EZ-bilirubin (confi gurational photoisomers), and lumirubin (LR, structural photoisomer) [1]. EZ-bilirubin, by absorbing a photon of radiation, then is also transformed to LR. LR molecules are considerably more hydrophilic than ZZ-bilirubin molecules and its confi gurational isomers, and so LR dissolves well in water and is easily excreted from the body. ZZ-bilirubin molecules from the blood replace the isomerized bilirubin in the subcutaneous tissue and also undergo isomerization. Treatment is accompanied by periodic blood draws from the patient, and is continued until the blood bilirubin level is reduced to a value safe for the patient.Currently in medical practice there is no standardized method for phototherapy to treat neonatal jaundice. Phototherapy systems are diverse, as are the radiation sources used in them (fl uorescent lamps, halogen lamps, light-emitting diodes, etc.). In a number of cases, modern phototherapy is characterized by low effi ciency (slow reduction of the blood bilirubin level), and is associated with harmful side effects of overdosing [2]. A large number of theoretical and experimental papers have been published that are devoted to selecting the light source that will provide the highest rate of decrease in the ZZbilirubin content in skin tissue and blood of newborns with the lowest possible exposure dose. The results of most of the studies show th...

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confidence: 98%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Method for Estimating Bilirubin Isomerization Efficiency in Phototherapy to Treat Neonatal Jaundice

Lisenko

Kugeiko

2014

J Appl Spectrosc

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Calculation of the Optical Properties of Pentaerythritol Tetranitrate–Cobalt Nanoparticle Composites

et al. 2015

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Aduev, a UDC 535.36:620.3 and M. V. Ananyeva bCoeffi cients of transmission, absorption, and refl ectance of a composite based on pentaerythritol tetranitrate and spherical cobalt nanoparticles were calculated as functions of sample thickness and mass fraction of metal particles using Mie theory and radiative transfer theory (spherical harmonics method). It was shown that the coeffi cient of refl ectance of light normally incident on the half-space with the plane boundary increased as the Co-particle radius increased. This was because of increases of both the single-scattering albedo and the modulus of the scattering anisotropy factor. The developed method for calculating optical properties of composites based on a transparent matrix with metal nanoparticle inclusions could be used to process experimental light-scattering spectroscopy data.Introduction. The development of light-scattering spectroscopic methods is a high-priority problem because a large number of natural and industrial objects, the rapid and non-destructive monitoring of which is required for technical applications, can both scatter and absorb light. Several studies focused on the solution of this problem [1][2][3][4][5]. A method for obtaining diffuse-refl ectance and transmission spectra was developed and applied to the study of gastric mucous membranes [1]. A method for obtaining diffuse-refl ectance spectra of biological samples using an integrating sphere was proposed [2]. The researchers showed that the developed spectrometer could be used to investigate human skin samples. The use of scattering spectroscopic methods for blood analysis was examined [3,4]. Thus, a rapid blood-analysis method with determination of the concentrations of hemoglobin and its derivatives that did not require pretreatment, including centrifugation, was proposed [3]. The method was based on obtaining diffuse-transmission spectra and their approximation using a Monte-Carlo method and several approximations describing blood spectral properties as a function of composition. These ideas were further developed [4] by proposing and validating a non-invasive blood-analysis method based on obtaining the diffuse-refl ectance spectrum of lip mucous membrane using a fi ber-optic detector. A method for interpreting multi-spectral images of biological tissues that could produce the spatial distribution of their biophysical parameters was proposed [5].Theoretical methods for processing results are insuffi ciently developed and limit the use of a photometric sphere and other procedures for studying light-scattering systems. The principal theories such as dual-beam and diffusion approximations invoke several assumptions that muddle the results and make them more qualitative than quantitative. The Monte-Carlo method often requires long computations to reach the required accuracy. Therefore, theoretical methods for processing experimental results, including the use of radiative transfer theory, are needed in order to utilize as much as possible the advantages of optical measurements w...

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Blood species identification using Near-Infrared diffuse transmitted spectra and PLS-DA method

Zhang

Sun

et al. 2016

Infrared Physics & Technology

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Rapid Analysis of Hemoglobins in Whole Blood by a Light Scattering Method

Cited by 3 publications

References 13 publications

Method for Estimating Bilirubin Isomerization Efficiency in Phototherapy to Treat Neonatal Jaundice

Method for Estimating Bilirubin Isomerization Efficiency in Phototherapy to Treat Neonatal Jaundice

Calculation of the Optical Properties of Pentaerythritol Tetranitrate–Cobalt Nanoparticle Composites

Blood species identification using Near-Infrared diffuse transmitted spectra and PLS-DA method

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