Rapid analysis and identification of dianthrone glycosides in Polygoni Multiflori Caulis based on enrichment of macroporous absorbent resin and UPLC‐Q‐TOF‐MS/MS

Abstract

Introduction Polygoni Multiflori Caulis (PMC) has been used as a traditional Chinese medicine for a long time in China. However, hepatotoxic events of PMC have been reported in recent years, but the potential toxic compounds have remained unclear. Dianthrones as the secondary plant metabolites were revealed to potential hepatotoxicity in a previous study. However, no reports focused on dianthrones in PMC. Objective In the quest for exploring potential hepatotoxic compounds in PMC, the aim of this work was to undertake a comprehensive characterisation of dianthrones in PMC. Methods A simple and effective macroporous absorbent resin column chromatography method was established in this study to enrich the minor dianthrones from PMC extracts. Exploration and characterisation of dianthrones in PMC was conducted by an ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐QTOF‐MS/MS) method and information dependent acquisition (IDA) mode. The aglycones of dianthrone glycosides were further verified by acid hydrolysis method. Results Seventy‐two dianthrone glycosides and their five aglycones were discovered and tentatively characterised in PMC for the first time, of which 29 dianthrones were identified as potential new compounds. Dianthrone glycosides could be classified into three types according to their aglycone structures, and their fragmentation pathway rules and diagnosed ions were also summarised comprehensively. Conclusion This was the first comprehensive investigation on dianthrones in PMC. The result would help to fully understand the phytochemical constituents and toxic components in PMC, and highlight the need for further toxicological investigations of the dianthrones in PMC due to their potential hepatotoxicity correlation.

Exploring potential mechanism of ciwujia tablets for insomnia by UPLC-Q-TOF-MS/MS, network pharmacology, and experimental validation

“…Due to its high selectivity and sensitivity, the UPLC-Q-TOF-MS/MS technique is considered an appropriate means to accurately analyze the ingredients of CWT. Recently, this technique has been used to analyze TCM and other natural medicines ( Bo et al, 2021 ; Cheng et al, 2021 ; El-Sabagh et al, 2021 ; Lin et al, 2021 ), revealing details of unknown components that are considered groundbreaking discoveries in the field. In this experiment, the UPLC-Q-TOF-MS/MS technique was adopted to analyze the ingredients of CWT, and the methodology was combined with other network pharmacology techniques used to examine the potential targets and metabolic pathways of CWT ingredients in the treatment of insomnia.…”

Beware of the Potential Risks for Polygoni Multiflori Caulis-Induced Liver Injury

“…Polygoni Multiflori Caulis (PMC) is the dried stem of PM, which is also included in ChP (Zhao et al, 2013;Wang et al, 2017;Bo et al, 2021). According to ChP, PMC is used to treat sleeping disorder, rheumatic arthralgia, and skin pruritus.…”

“…According to ChP, PMC is used to treat sleeping disorder, rheumatic arthralgia, and skin pruritus. Pharmacological studies have indicated its hypnosis, antidiabetic, and antioxidant effects (Bo et al, 2021). Although PMC has a larger amount of usage in clinical practice than RPM and PMP, fewer cases on liver injury induced by PMC are reported.…”