Rapid amplification for the detection of Mycobacterium tuberculosis using a non-contact heating method in a silicon microreactor based thermal cycler

Ke, Cathy; Berney, Helen; Mathewson, A.; Sheehan, Michelle M.

doi:10.1016/j.snb.2004.04.083

Cited by 25 publications

(16 citation statements)

References 23 publications

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“…Sputum smear microscopy has been used for TB diagnosis with relatively lower sensitivity in resource-limited settings [90,91]. Sheehan et al reported a polymerase chain reaction (PCR)-based Mycobacterium tuberculosis diagnostic device designed as a microfluidic device for DNA amplification [92]. They described a noncontact thermo-cycling approach using low power halogen lamp for DNA amplification from the pathogen.…”

Section: Current Challenges and Future Perspectivesmentioning

confidence: 99%

Nano/Microfluidics for diagnosis of infectious diseases in developing countries

Lee

Kim

Chung

et al. 2010

Advanced Drug Delivery Reviews

306

220

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Nano/microfluidic technologies are emerging as powerful enabling tools for diagnosis and monitoring of infectious diseases in both developed and developing countries. Miniaturized nano/microfluidic platforms that precisely manipulate small fluid volumes can be used to enable medical diagnosis in a more rapid and accurate manner. In particular, these nano/microfluidic diagnostic technologies are potentially applicable to global health applications, because they are disposable, inexpensive, portable, and easy-to-use for detection of infectious diseases. In this paper, we review recent developments in nano/microfluidic technologies for clinical point-of-care applications at resource-limited settings in developing countries.

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Section: Current Challenges and Future Perspectivesmentioning

confidence: 99%

Nano/Microfluidics for diagnosis of infectious diseases in developing countries

Lee

Kim

Chung

et al. 2010

Advanced Drug Delivery Reviews

306

220

View full text Add to dashboard Cite

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“…106 The device uses a low-cost and low-power halogen lamp heat source that, together with the need for smaller volumes of required sample and reagents, will allow the reduction of the thermocycling step from hours to minutes and therefore enhance usefulness for the development of a portable field device. 10 Given the extreme ease of contamination with the TB pathogen, especially within health care settings, there is a strong demand for a rapid TB test in suspected decedents, as the most common test requires one month (at least) for results.…”

Section: Tuberculosismentioning

confidence: 99%

Biosensors as rapid diagnostic tests for tropical diseases

Teles

Tavira

Fonseca

2010

Critical Reviews in Clinical Laboratory Sciences

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Effective diagnosis of infectious pathogens is essential for disease identification and subsequent adequate treatment, to prevent drug resistance and to adopt suitable public health interventions for the prevention and control of epidemic outbreaks. Particular situations under which medical diagnostics operate in tropical environments make the use of new easy-to-use diagnostic tools the preferred (or even unique) option. These diagnostic tests and devices, usually based on biosensing methods, are being increasingly exploited as promising alternatives to classical, "heavy" lab instrumentation for clinical diagnosis, allowing simple, inexpensive and point-of-care testing. However, in many developing countries the lack of accessibility and affordability for many commercial diagnostic tests remains a major cause of high disease burden in such regions. We present a comprehensive overview about the problems of conventional medical diagnosis of infectious pathologies in tropical regions, while pointing out new methods and analytical tools for in-the-field and decentralized diagnosis of current major infectious tropical diseases. The review includes not only biosensor-based rapid diagnostic tests approved by regulatory entities and already commercialized, but also those at the early stages of research.

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“…Available methods that can provide the required temperature variations include thin-film heating elements made of platinum (Pt), indium tin oxide (ITO), and non-metallic polysilicon Kaigala et al 2008;Liao et al 2005); heating/cooling blocks (Ross and Locascio 2002;Balss et al 2004;Sommer et al 2007); embedded resistive wires or silver-filled epoxy Vigolo et al 2010); external Peltier modules (Ross and Locascio 2002;Huber and Santiago 2007;Liu et al 2002); localized convective heating (Lee et al 2004, Wang et al 2003; infrared, visible, or microwave radiation (Duhr and Braun 2006;Krishnan et al 2009;Oda et al 1998;Ke et al 2004;Issadore et al 2009); chemical reactions (Guijt et al 2003); and Joule heating (Sommer et al 2007;Erickson et al 2003). Among the abovementioned methods, heating/cooling blocks (Ross and Locascio 2002;Balss et al 2004;Sommer et al 2007), Peltier elements (Huber and Santiago 2007;Matsui et al 2007), and Joule heating in a variable cross-section microchannel (Ross and Locascio 2002;Sommer et al 2007) have been used for analyte preconcentration with TGF.…”

Section: Introductionmentioning

confidence: 99%

Optothermal sample preconcentration and manipulation with temperature gradient focusing

Akbari

Bahrami

Sinton

2011

Microfluid Nanofluid

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In this article, we present an optothermal analyte preconcentration method based on temperature gradient focusing. This approach offers a flexible, noninvasive technique for focusing and transporting charged analytes in microfluidics using light energy. The method uses the optical field control provided by a digital projector as established for particle manipulation, to achieve analogous functionality for molecular analytes for the first time. The optothermal heating system is characterized and the ability to control of the heated zone location, size, and power is demonstrated. The method is applied to concentrate a sample model analyte, along a microcapillary, resulting in almost 500-fold local concentration increase in 15 min. Optically controlled upstream and downstream transport of a focused analyte band is demonstrated with a heater velocity of *170 lm/min.

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Rapid amplification for the detection of Mycobacterium tuberculosis using a non-contact heating method in a silicon microreactor based thermal cycler

Cited by 25 publications

References 23 publications

Nano/Microfluidics for diagnosis of infectious diseases in developing countries

Nano/Microfluidics for diagnosis of infectious diseases in developing countries

Biosensors as rapid diagnostic tests for tropical diseases

Optothermal sample preconcentration and manipulation with temperature gradient focusing

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