Rapid alcohol determination in plasma and urine by column liquid chromatography with biosensor detection

Lidén, Helena; Vijayakumar, A; Gorton, Lo; Markó-Varga, György

doi:10.1016/s0731-7085(98)00077-6

Cited by 51 publications

(23 citation statements)

References 44 publications

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“…Since the oxidase step involves biological recognition and not electrical transduction, it was considered plausible that G2 would contribute with beneficial activating effects on P2O, but not to the background current. The graphite powder (from Fluka) used in most of the previous publications concerning carbon paste from our laboratory [12,[15][16][17]19], including this work, was shown to give rise to medium sensitivity and background current [41], and was thus used as a comparison to the graphite powders mentioned above. The responses to glucose and hydrogen peroxide as well as the observed background currents are listed in Table 2.…”

Section: Graphitesmentioning

confidence: 99%

“…Of particular interest in this context of finding reliable enzyme based detection systems for monitoring and control of ethanol bioprocesses based on lignocellulose hydrolyzates, has been the construction of an alcohol sensor based on alcohol oxidase coimmobilized with horseradish peroxidase [16][17][18][19]. A similar approach was therefore used to construct a sensor to monitor the various saccharides present in lignocellulose hydrolyzates by the use of the enzyme pyranose oxidase (P2O, EC 1.1.3.10) in carbon paste.…”

Section: Introductionmentioning

confidence: 99%

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Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

et al. 1998

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An amperometric biosensor for the detection of some monosaccharides was developed with co-immobilized pyranose oxidase (purified from the basidiomycete fungus Phanerochaete chrysosporium) and horseradish peroxidase in carbon paste. Due to rather low selectivity of pyranose oxidase, the biosensor could detect both predominating anomers of D-glucose as well as the substrates D-xylose, D-galactose, and d-gluconolactone. The best sensor performance was obtained from a carbon paste with covalently immobilized enzymes together with the additives lactitol and polyethylenimine, resulting in a sensitivity for glucose of 30.2 mA cm ¹2 mM ¹1 . The enzyme modified electrode was investigated in an automated flow injection system at the operating potential of ¹50 mV (vs. Ag/AgCl) with an injection frequency of 40 h ¹1 . The biosensor was also integrated as the detection unit in a liquid chromatographic system for the detection of monosaccharides to show its potential use in real applications.

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Section: Graphitesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

et al. 1998

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“…Recently, new perspectives for the development of novel enzyme immobilization strategies for biosensor applications that use dendrimer are reported in the literature. [36][37][38][39][40][41] This article has been organized in two sections; initially various parameters effecting biosensor efficiency were optimized and characterized in batch system for both GOx and PyOx such as optimum pH, enzyme loading, linear range, operational stability, and shelf life. In the next section, offline FIA experiments were investigated.…”

Section: Introductionmentioning

confidence: 99%

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Yüksel

Akin

Geyik

et al. 2011

Biotechnology Progress

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This article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer (50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R(2) = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R(2) = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements.

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“…Thereby, the accurate measurement of ethanol concentration in human breath and blood is very important for medicine and lawsuit. Chromatographic and spectrometric methods were usually employed to achieve the tasks mentioned above, especially for complicated samples, such as serum [1,2]. However, those methods always suffered from several shortcomings such as relatively expensive instrumentation, time consuming, sophisticated operation procedures and laborious sample pre-treatment, and are being substituted by enzyme based electrochemical biosensors because of their inexpensive, rapid, reliable and specific characteristics [3 -5].…”

Section: Introductionmentioning

confidence: 99%

Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode

et al. 2009

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An amperometric ethanol biosensor was fabricated by integration of alcohol dehydrogenase (ADH) with meldolas blue (MB)/ordered mesoporous carbon (OMC) composite modified glassy carbon electrode (MB/OMC/GCE). The MB/OMC/GCE was highly sensitive for nicotinamide adenine dinucleotide (NADH) measurement (9.1 AE 0.25 mA/ mM) and gave a low detection limit of 0.21 AE 0.02 mM. The ethanol biosensor exhibited a wide linear range up to 6 mM with a lower detection limit of 19.1 AE 0.58 mM as well as a high sensitivity of 34.58 AE 2.43 nA/mM without suffering any interference from some common electroactive compounds.

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Rapid alcohol determination in plasma and urine by column liquid chromatography with biosensor detection

Cited by 51 publications

References 44 publications

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode

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