Rapid adaptive and acute stimulatory responses to low salinity stress in Pacific white shrimp (Litopenaeus vannamei): Insights from integrative transcriptomic and proteomic analysis

Lu, Huijie; Chen, Wenchun; Peng, Kai; Huang, Minwei; Zhao, Jichen; Chen, Xiaoying; Sun, Yuping; Ruan, Zhuohao; Li, Chaozheng; Liu, Ding; Liu, Zhenxing; Li, Huo; Zhang, Yong; Huang, Wen

doi:10.1016/j.cbd.2023.101149

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…Although previous studies have examined the adaptive transcriptomic response of F. chinensis to low salinity [3,14,31,32], we used LC-MS-based metabolomics to determine its rapid adaptive and acute stimulatory response to low-salinity stress. Our study indicated that some DEMs were steadily down-regulated at the early stage of stress in S3 and S7, and these DEMs were more than the up-regulated metabolites during salinity stress, suggesting that acute low salinity exposure induced a drastic change in the metabolite content of the intestine.…”

Section: Discussion 41 Low-salinity Stress Induced Micromorphic and M...mentioning

confidence: 99%

“…The expression of up-regulated L-Pcar can increase the levels of palmitate and triacylglycerides and decrease the levels of free cholesterol, which can modulate signal transduction pathways [39]. This suggests that shrimp require extra energy from fatty acids to adapt and survive under low-salinity stress [32,39].…”

Section: Effects Of Low-salinity Stress On Fatty Acid and Retinol Met...mentioning

confidence: 99%

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Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine of Fenneropenaeus chinensis

Tian,

Wang,

Gao

et al. 2024

Animals

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Metabolomics has been used extensively to identify crucial molecules and biochemical effects induced by environmental factors. To understand the effects of acute low-salinity stress on Fenneropenaeus chinensis, intestinal histological examination and untargeted metabonomic analysis of F. chinensis were performed after exposure to a salinity of 15 ppt for 3, 7, and 14 d. The histological examination revealed that acute stress resulted in most epithelial cells rupturing, leading to the dispersion of nuclei in the intestinal lumen after 14 days. Metabolomics analysis identified numerous differentially expressed metabolites (DEMs) at different time points after exposure to low-salinity stress, in which some DEMs were steadily downregulated at the early stage of stress and then gradually upregulated. We further screened 14 overlapping DEMs, in which other DEMs decreased significantly during low-salinity stress, apart from L-palmitoylcarnitine and vitamin A, with enrichments in phenylalanine, tyrosine and tryptophan biosynthesis, fatty acid and retinol metabolism, and ABC transporters. ABC transporters exhibit significant abnormalities and play a vital role in low-salinity stress. This study provides valuable insights into the molecular mechanisms underlying the responses of F. chinensis to acute salinity stress.

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Section: Discussion 41 Low-salinity Stress Induced Micromorphic and M...mentioning

confidence: 99%

Section: Effects Of Low-salinity Stress On Fatty Acid and Retinol Met...mentioning

confidence: 99%

Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine of Fenneropenaeus chinensis

Tian,

Wang,

Gao

et al. 2024

Animals

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Comprehensive analysis of butyric acid impact on immunology, histopathology, gene expression, and metabolomic responses in pacific shrimp experiencing cold stress

Yohana,

Ray,

Yang

et al. 2024

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Regulatory Effects of Myo‐Inositol on Carbohydrate Metabolism and Low‐Salinity Adaptation in Pacific White Shrimp (Penaeus vannamei)

Lin,

Han,

et al. 2024

Aquaculture Nutrition

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This study investigated how dietary myo‐inositol affects osmoregulation, carbohydrate and lipid metabolism, immunity, and antioxidant properties in Penaeus vannamei under long‐term low‐salinity conditions. A two‐factor (2 × 3) orthogonal approach was used with three different concentrations of dietary myo‐inositol (0, 1,200, and 2,000 mg/kg) at two dietary carbohydrate levels: normal carbohydrate or high carbohydrate (HC). Six experimental diets were formulated, and a feeding trial was carried out for 42 days. The interaction of myo‐inositol with an HC diet significantly improved shrimp survival, weight gain, and specific growth rates. Dietary myo‐inositol increased the contents of Na+, K+, and Ca2+ in the serum and reduced the ion loss caused by low‐salinity stress. In addition, a lack of myo‐inositol can lead to loosening of gill filament connections and thinning or disappearance of the cuticle. However, myo‐inositol supplementation protected the structural and functional integrity of the shrimp gills and significantly enhanced the ion transport capacity (P < 0.05). Two‐factor analysis showed that myo‐inositol concentration was affected by dietary carbohydrate levels, and an HC diet enhanced myo‐inositol biosynthesis in shrimp gills (P < 0.05). The concentrations of myo‐inositol are highest in the gills and lowest in the liver and pancreas. Moreover, dietary myo‐inositol can improve the immune function and antioxidant capacity of P. vannamei. The results also indicated that under sustained low‐salinity stress, high levels of carbohydrates cannot relieve the stress caused by low‐salinity conditions and can even affect the healthy growth of P. vannamei. Under HC levels, adding dietary myo‐inositol significantly improved glycogen and lipid deposition in hepatopancreas tissue and enhanced carbohydrate utilization and osmotic adjustment ability in P. vannamei under chronic low‐salinity stress.

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Rapid adaptive and acute stimulatory responses to low salinity stress in Pacific white shrimp (Litopenaeus vannamei): Insights from integrative transcriptomic and proteomic analysis

Cited by 3 publications

References 54 publications

Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine of Fenneropenaeus chinensis

Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine of Fenneropenaeus chinensis

Comprehensive analysis of butyric acid impact on immunology, histopathology, gene expression, and metabolomic responses in pacific shrimp experiencing cold stress

Regulatory Effects of Myo‐Inositol on Carbohydrate Metabolism and Low‐Salinity Adaptation in Pacific White Shrimp (Penaeus vannamei)

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