Rapid adaptation of predation resistance in bacteria isolated from a seawater microcosm

Mathisen, Peter; Thelaus, Johanna; Luna, Sara Sjöstedt de; Andersson, Agneta

doi:10.3354/ame01802

Cited by 8 publications

(5 citation statements)

References 45 publications

(48 reference statements)

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“…However, at both our sampling sites, the salinity was <5 psu, indicating that other environmental factors could have been of importance. Previous studies have shown that Duganella as well as Shewanella occur in both freshwater and brackish water ( Duganella : Rappé et al, 2000 ; Lin et al, 2019 ) ( Shewanella : Deng et al, 2014 ; Mathisen et al, 2016 ; Castillo et al, 2018 ). Both bacteria were isolated from brackish water, at a salinity of 0.9 and 4.4 psu, respectively, and the experiment was performed at a salinity of 3.5–4 psu.…”

Section: Discussionmentioning

confidence: 99%

Response of Coastal Shewanella and Duganella Bacteria to Planktonic and Terrestrial Food Substrates

et al. 2022

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Global warming scenarios indicate that in subarctic regions, the precipitation will increase in the future. Coastal bacteria will thus receive increasing organic carbon sources from land runoff. How such changes will affect the function and taxonomic composition of coastal bacteria is poorly known. We performed a 10-day experiment with two isolated bacteria: Shewanella baltica from a seaside location and Duganella sp. from a river mouth, and provided them with a plankton and a river extract as food substrate. The bacterial growth and carbon consumption were monitored over the experimental period. Shewanella and Duganella consumed 40% and 30% of the plankton extract, respectively, while the consumption of the river extract was low for both bacteria, ∼1%. Shewanella showed the highest bacterial growth efficiency (BGE) (12%) when grown on plankton extract, while when grown on river extract, the BGE was only 1%. Duganella showed low BGE when grown on plankton extract (< 1%) and slightly higher BGE when grown on river extract (2%). The cell growth yield of Duganella was higher than that of Shewanella when grown on river extract. These results indicate that Duganella is more adapted to terrestrial organic substrates with low nutritional availability, while Shewanella is adapted to eutrophied conditions. The different growth performance of the bacteria could be traced to genomic variations. A closely related genome of Shewanella was shown to harbor genes for the sequestration of autochthonously produced carbon substrates, while Duganella contained genes for the degradation of relatively refractive terrestrial organic matter. The results may reflect the influence of environmental drivers on bacterial community composition in natural aquatic environments. Elevated inflows of terrestrial organic matter to coastal areas in subarctic regions would lead to increased occurrence of bacteria adapted to the degradation of complex terrestrial compounds with a low bioavailability.

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Section: Discussionmentioning

confidence: 99%

Response of Coastal Shewanella and Duganella Bacteria to Planktonic and Terrestrial Food Substrates

et al. 2022

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“…The ability to rapidly gain predation resistance traits has been reported to be plastic and common among bacteria (Mathisen et al, 2016). Because the loss-of-function mutants of yjiC in predation-resistant B. licheniformis strains could not be obtained, an alternative strategy was used to investigate whether the predation resistance could be induced in the predation-sensitive strains Y071 and Y322 under the predatory pressure (Fig.…”

Section: Resultsmentioning

confidence: 99%

Bacillus licheniformis escapes from Myxococcus xanthus predation by deactivating myxovirescin A through enzymatic glucosylation

Zhang

Wang

et al. 2019

Environmental Microbiology

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Summary Myxococcus xanthus kills susceptible bacteria using myxovirescin A (TA) during predation. However, whether prey cells in nature can escape M. xanthus by developing resistance to TA is unknown. We observed that many field‐isolated Bacillus licheniformis strains could survive encounters with M. xanthus, which was correlated to their TA resistance. A TA glycoside was identified in the broth of predation‐resistant B. licheniformis J32 co‐cultured with M. xanthus, and a glycosyltransferase gene (yjiC) was up‐regulated in J32 after the addition of TA. Hetero‐expressed YjiC‐modified TA to a TA glucoside (TA‐Gluc) by conjugating a glucose moiety to the C‐21 hydroxyl group, and the resulting compound was identical to the TA glycoside present in the co‐culture broth. TA‐Gluc exhibited diminished bactericidal activity due to its weaker binding with LspA, as suggested by in silico docking data. Heterologous expression of the yjiC gene conferred both TA and M. xanthus‐predation resistance to the host Escherichia coli cells. Furthermore, under predatory pressure, B. licheniformis Y071 rapidly developed predation resistance by acquiring TA resistance through the overexpression of yjiC and lspA genes. These results suggest that M. xanthus predation resistance in B. licheniformis is due to the TA deactivation by glucosylation, which is induced in a predator‐mediated manner.

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“…We tested whether the predation-pressure on bacteria was a key factor for the occurrence of PRB. Predation-pressure on bacteria was calculated in two different ways: Ratio between the carbon biomasses of heterotrophic protozoa+eukaryotes and heterotrophic bacteria: Ratio between the carbon biomasses of heterotrophic protozoa and heterotrophic bacteria 72 , 73 : …”

Section: Methodsmentioning

confidence: 99%

Predators and nutrient availability favor protozoa-resisting bacteria in aquatic systems

Andersson

Ahlinder

Mathisen

et al. 2018

Sci Rep

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The long co-existence of bacteria and protozoa has led to the development of bacterial protozoa resistance strategies, which are suggested to serve as drivers for the evolution of pathogenic bacteria. However, the ecological mechanisms underpinning selection for protozoa-resistance in aquatic bacteria are poorly known. To assess the role of nutrient availability and predation-pressure on selection for protozoa-resisting bacteria (PRB), an enrichment-dilution experiment was designed using laboratory microcosms containing natural lake water. PRB was monitored by screening 16S rRNA amplicon sequence data for reads assigned to bacteria that previously has been shown to resist degradation by amoebae. To estimate the effects of the microbial food web dynamics (microscopy of; heterotrophic bacteria, phytoplankton, protozoa and rotifers) and physicochemical variables on the PRB abundance in the study system, a joint species distribution modelling approach was used. The predation-pressure (ratio between predator and bacterial biomass) had a positive effect on the abundance of the PRB genus Mycobacterium, while perturbation (enrichment and dilution) favored the PRB genus Pseudomonas that dominated the bacterial community in the disturbed systems. Our results show that PRB with different ecological strategies can be expected in water of high and intermediate nutrient levels and after major disturbances of an aquatic system.

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Rapid adaptation of predation resistance in bacteria isolated from a seawater microcosm

Cited by 8 publications

References 45 publications

Response of Coastal Shewanella and Duganella Bacteria to Planktonic and Terrestrial Food Substrates

Response of Coastal Shewanella and Duganella Bacteria to Planktonic and Terrestrial Food Substrates

Bacillus licheniformis escapes from Myxococcus xanthus predation by deactivating myxovirescin A through enzymatic glucosylation

Predators and nutrient availability favor protozoa-resisting bacteria in aquatic systems

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