Rapid adaptation of <i>Chironomus yoshimatsui</i> to acetylcholinesterase inhibitors (pyraclofos and pirimicarb) in a multi-generation study

Ishimota, Makoto; Tajiki-Nishino, Risako; Fukuyama, Tomoki; Tomiyama, Naruto

doi:10.1080/03601234.2019.1708165

Cited by 11 publications

(17 citation statements)

References 43 publications

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“…In our previous study, the recovery rates for pirimicarb at 0.5 µg/L and 160 mg/L were 95% to 104% and the relative standard deviations (RSDs) were 1% to 2% in the same test medium and the chemical was very stable under these test conditions for 48 h (Ishimota et al, 2020a). Thus, the analytical accuracy, variation, and stability under the test conditions were checked only for diazepam in the present study.…”

Section: Chemical Analysismentioning

confidence: 85%

“…The chemicals were quanti ed by liquid chromatography-mass spectrometry (LC-MS/MS) at a ow rate of 0.3 mL/min using a column at 40 °C (LC: 1290 HPLC; MS/MS: 6460 Triple Quad LC-MS/MS, Agilent Technologies, CA, USA; column: Acquity UPLC HSS T3, 1.8 μm × 2.1 mm × 100 mm, Waters, MA, USA). The operating conditions and mobile phase were in accordance with the procedure outlined by Ishimota et al (2020a). The monitored precursor/product ions for pirimicarb and diazepam were at 239.1/72.1 and 285.0/193.1 m/z, respectively.…”

Section: Chemical Analysismentioning

confidence: 99%

“…In our previous multi-generational study of tolerance to chemicals in chironomids, we used < 24-h-old larvae; however, we used the > 24-h and < 48-h-old (hereafter, < 48-h-old) larvae for all experiments in the present study because the size of this aged larvae was large enough to detect each of the mRNA levels, whereas that of the < 24-h-old larvae was not adequate (Ishimota et al, 2020a). Chironomid larvae (< 48h-old) were exposed to low effective concentrations of chemicals and were cultured until an appropriate number of egg masses was obtained for each generation ( ve successive generations: F0-F4) (Fig.…”

Section: Multi-generational Experimentsmentioning

confidence: 99%

“…Pulsed exposure to such chemicals is expected for pharmaceutical chemicals because these chemicals enter the water via sewage e uent from urban areas, including domestic dwellings or hospitals, as well as sewage released to the aquatic environment when it rains (McLellan et al, 2018;Pascoe et al, 2003). To elucidate the realistic exposure of chemicals to aquatic organisms, we previously investigated whether short-term exposure (48 h) of successive ( rst and second) generations of Chironomus yoshimatsui (< 24-h-old) to acetylcholinesterase (AChE) inhibitor insecticides (pirimicarb and pyraclofos) would change the sensitivity over four generations (Ishimota et al, 2020a). The ndings from this previous study showed that the chironomids exposed to pirimicarb produced larvae that were less sensitive than those in the control group and the increased AChE activity might be related to this low sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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Generational sensitivity alteration in Chironomus yoshimatsui to carbamate and pharmaceutical chemicals and the effect on Catalase, CYP450, and hemoglobin gene transcription

Ishimota¹,

Tomiyama²

2021

Ecotoxicology

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To ascertain the tolerance mechanisms of aquatic organisms to arti cial chemicals, intergenerational sensitivity changes of Chironomus yoshimatsui to a carbamate pesticide (pirimicarb) and pharmaceutical chemical (diazepam) were investigated. The larvae (< 48-h-old) in each generation were exposed to both chemicals for 48 h and then the surviving chironomids were cultured until the fth generation (F0-F4) without chemical addition. The 48-h 50% effective concentration (EC 50 ) value of chironomids was determined for each generation. In the pirimicarb treatment group, the EC 50 values signi cantly increased in F3 and F4, and those in the diazepam treatment group slightly increased. Catalase, Cytochrome P450 and hemoglobin (Hb) mRNA levels were monitored to see whether these were related to the trans-generational sensitivity. Although the generalized linear model results showed that the sensitivity to diazepam was slightly increased in the diazepam treatment, we could not nd any mRNA levels related to sensitivity alteration. In contrast, the model approach showed that the chironomids exposed to pirimicarb trans-generationally became tolerant with increasing Hb mRNA levels. Therefore, they might decrease their oxidative chemical stress by modifying Hb gene transcription.

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Section: Chemical Analysismentioning

confidence: 85%

Section: Chemical Analysismentioning

confidence: 99%

Section: Multi-generational Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Generational sensitivity alteration in Chironomus yoshimatsui to carbamate and pharmaceutical chemicals and the effect on Catalase, CYP450, and hemoglobin gene transcription

Ishimota¹,

Tomiyama²

2021

Ecotoxicology

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View full text Add to dashboard Cite

show abstract

“…The chemicals were quanti ed by liquid chromatography-mass spectrometry (LC-MS/MS) at a ow rate of 0.3 mL/min using a column at 40 °C (LC: 1290 HPLC; MS/MS: 6460 Triple Quad LC-MS/MS, Agilent Technologies, CA, USA; column: Acquity UPLC HSS T3, 1.8 μm × 2.1 mm × mm, Waters, MA, USA). The operating conditions and mobile phase were in accordance with the procedure outlined by Ishimota et al (2020a). The monitored precursor/product ions for pirimicarb and diazepam were at 239.1/72.1 and 285.0/193.1 m/z, respectively.…”

Section: Chemical Analysismentioning

confidence: 99%

Generational Sensitivity Alteration in Chironomus Yoshimatsui to Carbamate and Pharmaceutical Chemicals and The Effect on Catalase, CYP450, and Hemoglobin Gene Transcription

Ishimota

Tomiyama

2021

Preprint

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To ascertain the tolerance mechanisms of aquatic organisms to artificial chemicals, intergenerational sensitivity changes of Chironomus yoshimatsui to a carbamate pesticide (pirimicarb) and pharmaceutical chemical (diazepam) were investigated. The larvae (< 48-h-old) in each generation were exposed to both chemicals for 48 h and then the surviving chironomids were cultured until the fifth generation (F0–F4) without chemical addition. The 48-h 50% effective concentration (EC50) value of chironomids was determined for each generation. In the pirimicarb treatment group, the EC50 values significantly increased in F3 and F4, and those in the diazepam treatment group slightly increased. Catalase, Cytochrome P450 and hemoglobin (Hb) mRNA levels were monitored to see whether these were related to the trans-generational sensitivity. Although the generalized linear model results showed that the sensitivity to diazepam was slightly increased in the diazepam treatment, we could not find any mRNA levels related to sensitivity alteration. In contrast, the model approach showed that the chironomids exposed to pirimicarb trans-generationally became tolerant with increasing Hb mRNA levels. Therefore, they might decrease their oxidative chemical stress by modifying Hb gene transcription.

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Chemical tolerance related to the ABC transporter gene and DNA methylation in cladocera (Daphnia magna)

Ishimota,

Kodama,

Tomiyama

et al. 2023

Environmental Toxicology

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We performed multigenerational tests to clarify the chemical tolerance mechanisms of a nontarget aquatic organism, Daphnia magna. We continuously exposed D. magna to a carbamate insecticide (pirimicarb) at lethal or sublethal concentrations (0, 3.8, 7.5, and 15 μg/L) for 15 generations (F0‐F14). We then determined the 48 h‐EC50 values and mRNA expression levels of acetylcholinesterase, glutathione S‐transferase, and ATP (Adenosine triphosphate)‐binding cassette transporter (ABCt) in neonates (<24 h old) from F0, F4, F9, and F14. To ascertain the effects of DNA methylation on pirimicarb sensitivity, we measured 5‐methylcytosine levels (DNA methylation levels) in neonates of parents in the last generation (F14). In addition, we cultured groups exposed to 0 and 7.5 μg/L (the latter of which acquired chemical tolerance to pirimicarb) with or without 5‐azacytidine (de‐methylating agent) and determined methylation levels and 48 h‐EC50 values in neonates (<24 h old) from the treated parents. The EC50 values (30.3–31.6 μg/L) in F14 of the 7.5 and 15 μg/L groups were approximately two times higher than that in the control (16.0 μg/L). A linear mixed model analysis showed that EC50 and ABCt mRNA levels were significantly increased with generational alterations; further analysis showed that the ABCt mRNA level was positively related to the EC50. Therefore, ABCt may be associated with altered pirimicarb sensitivity. In addition, the EC50 value and DNA methylation levels in pirimicarb‐tolerant clones decreased after exposure to 5‐azacytidine, suggesting that DNA methylation contributes to chemical tolerance. These findings improved our knowledge regarding the acquisition of chemical tolerance in aquatic organisms.

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Rapid adaptation of Chironomus yoshimatsui to acetylcholinesterase inhibitors (pyraclofos and pirimicarb) in a multi-generation study

Cited by 11 publications

References 43 publications

Generational sensitivity alteration in Chironomus yoshimatsui to carbamate and pharmaceutical chemicals and the effect on Catalase, CYP450, and hemoglobin gene transcription

Generational sensitivity alteration in Chironomus yoshimatsui to carbamate and pharmaceutical chemicals and the effect on Catalase, CYP450, and hemoglobin gene transcription

Generational Sensitivity Alteration in Chironomus Yoshimatsui to Carbamate and Pharmaceutical Chemicals and The Effect on Catalase, CYP450, and Hemoglobin Gene Transcription

Chemical tolerance related to the ABC transporter gene and DNA methylation in cladocera (Daphnia magna)

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