Rapid Actin Transport During Cell Protrusion

Zicha, Daniel; Dobbie, Ian M.; Holt, Mark R.; Monypenny, James; Soong, Daniel; Gray, Colin; Dunn, Graham

doi:10.1126/science.1082026

Cited by 158 publications

(145 citation statements)

References 9 publications

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“…Among these we should mention NCOA1 (nuclear receptor coactivator (1), a transcriptional coactivator belonging to the SRC family which is deregulated in breast and prostatic cancer and may potentiate gene expression by acting as a coactivator for nuclear hormone receptors and other transcription factors (TF) [44][45][46][47][48][49][50][51][52][53][54][55]; and ROCK2, a serine/threonine kinase member of the Rho pathway, involved in cell adhesion, migration, invasion, and mitosis [56][57][58][59], which may be a potential therapeutic target in human cancer cells and animal models [60][61][62][63][64][65][66][67][68].…”

Section: Discussionmentioning

confidence: 99%

Chromosome 2p gain in monoclonal B‐cell lymphocytosis and in early stage chronic lymphocytic leukemia

et al. 2012

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Recent studies have described chromosome 2p gain as a recurrent lesion in chronic lymphocytic leukemia (CLL). We investigated the 2p gain and its relationship with common prognostic biomarkers in a prospective series of 69 clinical monoclonal B-cell lymphocytosis (cMBL) and 218 early stage (Binet A) CLL patients. The 2p gain was detected by FISH in 17 patients (6%, 16 CLL, and 1 cMBL) and further characterized by single nucleotide polymorphism-array. Overall, unfavorable cytogenetic deletions, i.e., del(11)(q23) and del(17)(p13) (P 5 0.002), were significantly more frequent in 2p gain cases, as well as unmutated status of IGHV (P < 1 3 10 24 ) and CD38 (P < 1 3 10 24 ) and ZAP-70 positive expression (P 5 0.003). Furthermore, 2p gain patients had significantly higher utilization of stereotyped B-cell receptors compared with 2p negative patients (P 5 0.009), and the incidence of stereotyped subset #1 in 2p gain patients was significantly higher than that found in the remaining CLLs (P 5 0.031). Transcriptional profiling analysis identified several genes significantly upregulated in 2p gain CLLs, most of which mapped to 2p. Among these, NCOA1 and ROCK2 are known for their involvement in tumor progression in several human cancers, whereas among those located in different chromosomes, CAV1 at 7q31.1 has been recently identified to play a critical role in CLL progression. Thus, 2p gain can be present since the early stages of the disease, particularly in those cases characterized by other poor prognosis markers. The finding of genes upregulated in the cells with 2p gain provides new insights to define the pathogenic role of this lesion. Am. J. Hematol. 88:24-31, 2013. V

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Section: Discussionmentioning

confidence: 99%

Chromosome 2p gain in monoclonal B‐cell lymphocytosis and in early stage chronic lymphocytic leukemia

et al. 2012

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“…[29]. Briefly, we assume exponentially decaying concentration profiles for Arp2=3 and G-actin away from the leading edge, with decay lengths z arp [30] and D=v t , respectively, where D is the G-actin diffusion constant and v t is the transport velocity of monomers towards the surface [31]. We assume an inverted exponential with rise length z s for severing protein to model the nucleotide dependence of severing.…”

Section: Prl 99 058103 (2007) P H Y S I C a L R E V I E W L E T T E mentioning

confidence: 99%

Branching, Capping, and Severing in Dynamic Actin Structures

et al. 2007

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Branched actin networks at the leading edge of a crawling cell evolve via protein-regulated processes such as polymerization, depolymerization, capping, branching, and severing. A formulation of these processes is presented and analyzed to study steady-state network morphology. In bulk, we identify several scaling regimes in severing and branching protein concentrations and find that the coupling between severing and branching is optimally exploited for conditions {\it in vivo}. Near the leading edge, we find qualitative agreement with the {\it in vivo} morphology.Comment: 4 pages, 2 figure

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“…The quantification of small-amplitude fluctuations observed as a result of subtle metabolic-dependent remodelling in nonmuscle cells is a promising application of AFM in cell biology. Fluctuations in intracellular cytoskeletal structures at the protruding edge of motile cells have been observed using various optical techniques [55,56]. Although these activities can be optically detected, the quantification of nanoscaled events and their association with specific cellular mechanical activities is problematic, mainly because of the low resolution of the optical approaches.…”

Section: Mechanical Sensing Of Cellular Processesmentioning

confidence: 99%

AFM as a tool to probe and manipulate cellular processes

Lamontagne

Cuerrier

Grandbois

2007

Pflugers Arch - Eur J Physiol

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The exploration of molecular processes governing physiological functions has significantly benefited from the emergence of novel nanoscaled techniques. Atomic force microscopy in force measurement mode can be used to investigate a multitude of cellular events in individual living cells with great sensitivity. Precise regions of the plasma membrane can be examined in relation to particular signalling pathways activated by a mechanical stimulus. Similarly, subtle cellular movements induced by biochemical activation of specific membrane receptors can be detected in real time with excellent temporal and spatial resolution. The possibility to challenge locally and mechanically cell surface receptors also provides information on the control exerted by a cell over individual adhesion sites. Overall, this information is vital for an in-depth understanding of mechanisms related to cellular movement and morphological regulation. Lastly, atomic force microscope-based nanomanipulations on living cells have recently been proposed as a tool to influence and monitor cellular homeostasis by introducing specific molecular entities into or extracting them from the cytoplasm of individual cells. This review provides detailed examples on how such atomic force microscopy experiments can be conducted to investigate processes relevant to cell physiology.

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Rapid Actin Transport During Cell Protrusion

Cited by 158 publications

References 9 publications

Chromosome 2p gain in monoclonal B‐cell lymphocytosis and in early stage chronic lymphocytic leukemia

Chromosome 2p gain in monoclonal B‐cell lymphocytosis and in early stage chronic lymphocytic leukemia

Branching, Capping, and Severing in Dynamic Actin Structures

AFM as a tool to probe and manipulate cellular processes

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