Rapid acousto-optic focus tuning for improvement of imaging performance in confocal microscopy [Invited]

Szulzycki, Krzysztof; Savaryn, V.; Grulkowski, Ireneusz

doi:10.1364/ao.57.000c14

Cited by 14 publications

(6 citation statements)

References 30 publications

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“…To generate the refractive index distribution inside the complex medium, we used a piezoelectric cylindrical cavity. When filled with a liquid and driven on resonance (4 MHz), radial standing ultrasonic waves are obtained in the cavity 21 , which induce a periodic change in the liquid refractive index given by: 22 where corresponds to the static refractive index of the liquid, the amplitude of the refractive index change that depends on the driving amplitude voltage, the Bessel function of first kind, is the acoustic wave vector, and the driving frequency. This refractive index acts as a time-dependent gradient index of refraction (GRIN) lens 23 , 24 .…”

Section: Resultsmentioning

confidence: 99%

Enhanced light focusing inside scattering media with shaped ultrasound

Mestre-Torà,

Duocastella

2023

Sci Rep

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Light focusing is the primary enabler of various scientific and industrial processes including laser materials processing and microscopy. However, the scattering of light limits the depth at which current methods can operate inside heterogeneous media such as biological tissue, liquid emulsions, and composite materials. Several approaches have been developed to address this issue, but they typically come at the cost of losing spatial or temporal resolution, or increased invasiveness. Here, we show that ultrasound waves featuring a Bessel-like profile can locally modulate the optical properties of a turbid medium to facilitate light guiding. Supported by wave optics and Monte Carlo simulations, we demonstrate how ultrasound enhances light focusing a factor of 7 compared to conventional methods based on placing optical elements outside the complex medium. Combined with point-by-point scanning, images of samples immersed in turbid media with an optical density up to 15, similar to that of weakly scattering biological tissue, can be reconstructed. The quasi-instantaneous generation of the shaped-ultrasound waves, together with the possibility to use transmission and reflection architectures, can pave the way for the real-time control of light inside living tissue.

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Section: Resultsmentioning

confidence: 99%

Enhanced light focusing inside scattering media with shaped ultrasound

Mestre-Torà,

Duocastella

2023

Sci Rep

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“…When combined with color or time encoding, information from the two planes acquired in single or consecutive frames was extracted in a post-processing step 84,85 . In another example of confocal 72,86 and two-photon 59,79,87,88 microscopes, pixel-by-pixel multiplane capture has been made possible with an ultra-high-speed liquid lens and synchronized detection. In this case, the high z-focus translation allowed one or multiple axial scans to be performed during the pixel dwell time.…”

Section: Implications Of the Ultra-high-speed Variable Focus Optics I...mentioning

confidence: 99%

Variable optical elements for fast focus control

2020

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In this article, we survey recent developments in the emerging field of high-speed variable z-focus optical elements, which are driving important innovations in advanced imaging and materials processing applications. 3D biomedical imaging, high-throughput industrial inspection, advanced spectroscopies, and other optical characterization and materials modifications methods have made great strides forward in recent years due to precise and fast axial control of light. Three state-of-the-art key optical technologies that enable fast z-focus modulation are reviewed along with a discussion on the implications of what the new variable optical element developments entail and the resulting impact on technologically relevant applications. Box figure. Strategies for axial focus control using a variable optical element. a) Focusing with the varifocal lens as the last focusing element. b) Normal configuration for high-resolution imaging or tight focusing. The varifocal lens is placed at a conjugate plane of the high NA focusing lens.

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“…In some applications, such as fluorescence live cell imaging, it is important to decrease the imaging time in order to follow the behavior of cells. To reduce the imaging time, faster scanners can be chosen, such as resonant [11] and microelectromechanical Systems (MEMS) scanners [12,13], or acoustic scanners like tunable acousto-optic lenses [14][15][16]. One remarkable way is sparse sampling of the field of view (FOV) using compressed sensing methods to speed up the imaging and also reduce photobleaching [17,18], which is a substantial issue in fluorescence microscopy [19].…”

Section: Introductionmentioning

confidence: 99%

Utilizing phase-modulated Lissajous to enhance spatial and temporal resolution of laser scanning microscopy

Qazvini¹,

Latifi²,

Asadollahi³

2022

J. Opt.

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One of the main challenges in scanning microscopy is increasing the scanning speed without deteriorating the spatial resolution of the image. With Lissajous scanning, the image can be available in a fraction of the time since it gathers scattered data from the entire FOV. However, this method reduces the density of scan lines at the center of the image, thereby reducing the imaging resolution. This problem can be solved by phase modulating the motion function of the scanner; however, the density of lines created by such designs is not always controllable. In this paper, simple relationships for determining the desired density over a large area are presented for selecting the appropriate phase modulation, especially for microscopy applications. In addition, a confocal microscope was employed to image a biological sample using the proposed relations to obtain a scanning density two times higher than conventional Lissajous. The results demonstrated that the imaging speed can be significantly increased by using phase modulation accompanied by interpolation methods for image reconstruction.

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Rapid acousto-optic focus tuning for improvement of imaging performance in confocal microscopy [Invited]

Cited by 14 publications

References 30 publications

Enhanced light focusing inside scattering media with shaped ultrasound

Enhanced light focusing inside scattering media with shaped ultrasound

Variable optical elements for fast focus control

Utilizing phase-modulated Lissajous to enhance spatial and temporal resolution of laser scanning microscopy

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