Rapeseed oils high in single fatty acid contents for oleochemical uses

Röbbelen, G.; Kräling, K.

doi:10.1016/0926-6690(92)90032-q

Cited by 7 publications

(7 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether the microspore-derived embryoid system described can also be used for in vitro selection of high-stearic-acid (Knutzon et al 1992), high-oleic, or low-linolenic lines (Auld et al 1992, Robbelen andKraling 1993) needs to be demonstrated with appropriate cross progenies. Optimization of the in vitro culture conditions for maximum expression of desaturated fatty acids should also allow, in this case, a better distinction of lines with different contents of polyenoic fatty acids and should increase the selection efficiency for specific genotypes in segregating microspore-derived embryoid populations.…”

Section: Discussionmentioning

confidence: 99%

Selection in vitro for erucic‐acid content in segregating populations of microspore‐derived embryoids of Brassica napus

Albrecht¹,

Möllers²,

Röbbelen³

1995

Plant Breeding

View full text Add to dashboard Cite

Microspore culture of Brassica napus under optimized conditions leads to the regeneration of microspore-derived embryoids that, at the late cotyledonary stage, contain large amounts of storage lipids, equal or similar in composition to those found in seeds of the homozygous donor plants. At that stage, the microspore-derived embryoids are large enough to allow the dissection of one cotyledon under aseptic conditions and the determination cf its fatty-acid composition. The remaining part of the embryoid can be cultured further and regenerated to give a plant. This offers the possibility of early selection for fatty-acid composition in segregating populations of microspore-derived embryoids. In order to verify this hypothesis, embryoids were generated from microspores of F| plants derived from a cross between doubled haploid lines of the low-erucic-acid cv. 'Duplo' and the high-erucic-acid cv. 'Janetzki'. The contents of eicosenoic acid (C20: 1) and erucic acid (C22: 1) in the cotyledons and in the seeds derived from plants regenerated from the remaining parts of the embryoids were highly correlated (rs = 0.85**, P = 0.01). This indicates that, in breeding programmes for high erucic acid, the majority of the microspore-derived embryoids can be discarded at an early stage in vitro. Only microspore-derived embryoids with a high content of C20 : 1 +C22 : 1 in the cotyledons need to be transferred to the greenhouse. This report also deals with the addition of abscisic acid (ABA) to the embryoid culture medium to increase the correlation, and discusses the possible application of this system for the selection of high-oleic or low-linolenic types in corresponding microspore-derived embryoid populations.

show abstract

Section: Discussionmentioning

confidence: 99%

Selection in vitro for erucic‐acid content in segregating populations of microspore‐derived embryoids of Brassica napus

Albrecht¹,

Möllers²,

Röbbelen³

1995

Plant Breeding

View full text Add to dashboard Cite

show abstract

“…In general, rapeseed oils contain about 65% oleic acid (C18:1), 20% linoleic acid (C18:2), and 10% linolenic acid (C18:3). A balanced fatty acid mixture of C16/C18 chain lengths is good for nutritional uses, whereas oleochemical applications prefer vegetable oils that contain a single fatty acid only (Röbbelen & Kräling, 1993). Erucic acid has traditionally been the required constituent in rapeseed oil, but rapeseed varieties with high oleic acid have recently been developed (Röbbelen & Kräling, 1993).…”

mentioning

confidence: 99%

“…A balanced fatty acid mixture of C16/C18 chain lengths is good for nutritional uses, whereas oleochemical applications prefer vegetable oils that contain a single fatty acid only (Röbbelen & Kräling, 1993). Erucic acid has traditionally been the required constituent in rapeseed oil, but rapeseed varieties with high oleic acid have recently been developed (Röbbelen & Kräling, 1993). The high level of oleic acid and corresponding high degree of oxidative stability in oilseeds is valuable for high-cost uses in the manufacture of certain cosmetics and pharmaceuticals (Röbbelen & Kräling, 1993).…”

mentioning

confidence: 99%

“…Erucic acid has traditionally been the required constituent in rapeseed oil, but rapeseed varieties with high oleic acid have recently been developed (Röbbelen & Kräling, 1993). The high level of oleic acid and corresponding high degree of oxidative stability in oilseeds is valuable for high-cost uses in the manufacture of certain cosmetics and pharmaceuticals (Röbbelen & Kräling, 1993). The development of rapeseed cultivars with high oleic and low linolenic acid, which confers good low-temperature fluidity, is highly desirable for biodiesel Lee et al, 2010).…”

mentioning

confidence: 99%

“…Mutation breeding has become a successful tool for manipulating the composition of fatty acids in oilseeds (Bühsenschüz-Nothdurft et al, 1998;Röbbelen & Kräling, 1993;Savant & Kothekar, 2011). Ethyl methanesulfonate (EMS) has been shown to be one of the more potent chemical mutagens available (Jacobs, 1969).…”

mentioning

confidence: 99%

See 2 more Smart Citations

EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.)

Lee¹,

Kim²,

Jang³

et al. 2014

Korean Journal of Crop Science

View full text Add to dashboard Cite

Rapeseed (Brassica napus L.) oil with high oleic acid content is of great interest for both food and non-food uses. The 'Tamla' variety, characterized by oleic acid content of approximately 69%, was treated with 1% ethyl methane sulfonate (EMS) to induce mutations in the fatty acid biosynthesis pathway. M 1 plants were selfed and subsequent generations (M2, M3, and M4 mutants) were analyzed to identify mutants having increased levels of oleic acid. M 2 mutants showed oleic acid content ranging from 13.5% to 76.9% with some mutants (TR-458 and TR-544) having up to 74.7% and 76.9% oleic acid, which was an increase of nearly 5% and 7%, respectively, compared to untreated cv 'Tamla'. We selected two M3 mutants with >75% oleic acid content. One mutant (TR-458-2) had increased oleic acid (75.9%) and decreased linoleic acid (12.5%) and linolenic acid (4.4%) contents. The other (TR-544-1) showed increased oleic acid content (75.7%) and decreased linoleic acid (13.5%) and linolenic acid (3.3%) contents. The accumulation or reduction of oleic acid content in the selected M 4 mutants was also accompanied by a simultaneous decrease or increase in linoleic and linolenic acid contents. The high-oleic lines could be utilized further in breeding programs for improvement of rapeseed oil quality.

show abstract

Comparative Study of Fatty Acid Composition, Total Phenolics, and Antioxidant Capacity in Rapeseed Mutant Lines

Channaoui¹,

Hssaini²,

Velasco

et al. 2020

J Americ Oil Chem Soc

View full text Add to dashboard Cite

There is limited variability within rapeseed germplasm in Morocco. Induced mutation was recently used to generate novel genetic variability and develop mutant lines combining desirable traits. In this context, nine rapeseed promising advanced M2 mutants lines and the wild-type variety 'INRA-CZH2' were evaluated for their seed oil content, fatty acid composition, total phenolics content (TPC), and free radical scavenging activity (FRSA) determined by 2,2-diphenyl-1picrylhydrazyl (DPPH) and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. The results showed significant variability, among all mutants, in seed oil content (from 38.14 to 42.04%) and in fatty acids (SAFA = 5.49 -10.99%, MUFA = 50.33% -71.62%, PUFA = 22.89% -38.68%). The mutant H2M-5 exhibited the highest fraction of MUFA and lowest proportion of SAFA and PUFA, while the mutant H2M-4 showed the highest SAFA and PUFA amounts and lowest MUFA level. TPC varied from 2.16 to 4.35 mg GAE/100 g. The highest amount was found in the mutant H2M-1, which is about twice, compared to all other mutants and the wild-type variety. Free radical scavenging activity (FRSA) differed significantly among samples and the variations observed for DPPH and ABTS methods were 40.5-59.28% and 40.31-59.86%, respectively. FRSA was positively correlated to TPC in sampled oils (r = 0.801 and 0.802, p < 0.01). This is the first report emphasizing biochemical potentialities of rapeseed varieties and novel mutants in Morocco. H2M-1, H2M-4 and H2M-5 were proposed for rapeseed national breeding program as they showed higher levels in some biochemical traits of interest.

show abstract

Rapeseed oils high in single fatty acid contents for oleochemical uses

Cited by 7 publications

References 5 publications

Selection in vitro for erucic‐acid content in segregating populations of microspore‐derived embryoids of Brassica napus

Selection in vitro for erucic‐acid content in segregating populations of microspore‐derived embryoids of Brassica napus

EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.)

Comparative Study of Fatty Acid Composition, Total Phenolics, and Antioxidant Capacity in Rapeseed Mutant Lines

Contact Info

Product

Resources

About