Rapeseed calcineurin B-like protein CBL4, interacting with CBL-interacting protein kinase CIPK24, modulates salt tolerance in plants

Liu, Wu-Zhen; Deng, Min; Li, Liang; Yang, Bo; Li, Hongwei; Deng, Hui; Jiang, Yuan‐Qing

doi:10.1016/j.bbrc.2015.10.034

Cited by 23 publications

(12 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results clearly lend support to the involvement of the SOS pathway in K + nutrition under salt stress as well as the impact of ROS in K + uptake. The SOS pathway in K + nutrition would likely operate as follows: the Ca 2+ sensor CBL, isoform CBL4 (SOS3), recruits the kinase CIPK24 (SOS2) to the plasma membrane where CIPK24 activates SOS1 via phosphorylation to extrude Na + in exchange for H + influx (Liu et al, 2015). This pathway aids in inhibiting membrane depolarization under salt stress; a condition favorable for K + uptake.…”

Section: Mechanisms Favoring Root K+ Uptakementioning

confidence: 99%

The Role of Na+ and K+ Transporters in Salt Stress Adaptation in Glycophytes

et al. 2017

View full text Add to dashboard Cite

Ionic stress is one of the most important components of salinity and is brought about by excess Na+ accumulation, especially in the aerial parts of plants. Since Na+ interferes with K+ homeostasis, and especially given its involvement in numerous metabolic processes, maintaining a balanced cytosolic Na+/K+ ratio has become a key salinity tolerance mechanism. Achieving this homeostatic balance requires the activity of Na+ and K+ transporters and/or channels. The mechanism of Na+ and K+ uptake and translocation in glycophytes and halophytes is essentially the same, but glycophytes are more susceptible to ionic stress than halophytes. The transport mechanisms involve Na+ and/or K+ transporters and channels as well as non-selective cation channels. Thus, the question arises of whether the difference in salt tolerance between glycophytes and halophytes could be the result of differences in the proteins or in the expression of genes coding the transporters. The aim of this review is to seek answers to this question by examining the role of major Na+ and K+ transporters and channels in Na+ and K+ uptake, translocation and intracellular homeostasis in glycophytes. It turns out that these transporters and channels are equally important for the adaptation of glycophytes as they are for halophytes, but differential gene expression, structural differences in the proteins (single nucleotide substitutions, impacting affinity) and post-translational modifications (phosphorylation) account for the differences in their activity and hence the differences in tolerance between the two groups. Furthermore, lack of the ability to maintain stable plasma membrane (PM) potentials following Na+-induced depolarization is also crucial for salt stress tolerance. This stable membrane potential is sustained by the activity of Na+/H+ antiporters such as SOS1 at the PM. Moreover, novel regulators of Na+ and K+ transport pathways including the Nax1 and Nax2 loci regulation of SOS1 expression and activity in the stele, and haem oxygenase involvement in stabilizing membrane potential by activating H+-ATPase activity, favorable for K+ uptake through HAK/AKT1, have been shown and are discussed.

show abstract

Section: Mechanisms Favoring Root K+ Uptakementioning

confidence: 99%

The Role of Na+ and K+ Transporters in Salt Stress Adaptation in Glycophytes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…These two kinase families form a dynamic complex to plant responses to many environmental signals and in regulating ion fluxes under salt stress (Yu et al, 2014). In A. thaliana, CBL4 (orthologs of Unigene104333_CL32) (salt overly sensitively 3, SOS3) interacts with CIPK24 (SOS2, orthologs of Unige-ne82254_CL32), and this interaction activates the plasma membrane-localized SOS1 and vacuolar H + -ATPase to promote salt tolerance (Liu et al, 2015). The CBL10-CIPK24 (orthologs of Unigene91258_CL32 and Unigene82254_CL32) complex is associated with vacuolar compartments and functions in protecting shoots from salt stress (Kim et al, 2007), whereas the interaction of CBL1 or CBL9 (orthologs of Unigene91258_CL32) with CIPK23 phosphorylates and activates the K + channel AKT1 to modulate K + homeostasis (Grefen and Blatt, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…AKT1 is present in the roots of A. thaliana and is responsible for participating in the absorption and transport of K + (Lagarde et al, 1996). At present, there are many experiments to prove that the transgenic AKT1 gene can enhance plant salt tolerance and improve K + utilization efficiency (Ardie et al, 2010;Liu et al, 2015). AKT2 is mainly localized in phloem cells, and potassium starvation and exogenous ABA can increase its expression level (Deeken et al, 2002;Pilot et al, 2003aPilot et al, , 2003b.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Chrysanthemum lavandulifolium Response to Salt Stress and Overexpression a K+ Transport ClAKT Gene-enhanced Salt Tolerance in Transgenic Arabidopsis

He¹,

Liu²,

Pu³

et al. 2019

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Plant growth and development are significantly affected by salt stress. Chrysanthemum lavandulifolium is a halophyte species and one of the ancestors of chrysanthemum (C. ×morifolium). Understanding how this species tolerates salt stress could provide vital insight for clarifying the salt response systems of higher plants, and chrysanthemum-breeding programs could be improved. In this study, salt tolerance was compared among C. lavandulifolium and three chrysanthemum cultivars by physiological experiments, among which C. lavandulifolium and Jinba displayed better tolerance to salt stress than the other two cultivars, whereas Xueshan was a salt-sensitive cultivar. Using the transcriptome database of C. lavandulifolium as a reference, we used digital gene expression technology to analyze the global gene expression changes in C. lavandulifolium seedlings treated with 200 mm NaCl for 12 hours compared with seedlings cultured in normal conditions. In total, 2254 differentially expressed genes (DEGs), including 1418 up-regulated and 836 down-regulated genes, were identified. These DEGs were significantly enriched in 35 gene ontology terms and 29 Kyoto Encyclopedia of Genes and Genomes pathways. Genes related to signal transduction, ion transport, proline biosynthesis, reactive oxygen species scavenging systems, and flavonoid biosynthesis pathways were relevant to the salt tolerance of C. lavandulifolium. Furthermore, comparative gene expression analysis was conducted using reverse transcription polymerase chain reaction to compare the transcriptional levels of significantly up-regulated DEGs in C. lavandulifolium and the salt-sensitive cultivar Xueshan, and species-specific differences were observed. The analysis of one of the DEGs, ClAKT, an important K+ transport gene, was found to enable transgenic Arabidopsis thaliana to absorb K+ and efflux Na+ under salt stress and to absorb K+ under drought stress. The present study investigated potential genes and pathways involved in salt tolerance in C. lavandulifolium and provided a hereditary resource for the confinement of genes and pathways responsible for salt tolerance in this species. This study provided a valuable source of reference genes for chrysanthemum cultivar transgenesis breeding.

show abstract

“…Furthermore, OsCIPK24 (SOS2) in Chr6:M-QTL3 and OsCIPK10 in Chr3:M-QTL2 were upregulated in the seedlings (Table S6, Fig.4). CIPK (CBL-Interacting Protein Kinases) pathway has emerged as a main signaling pathway and adjusts the salt tolerance in rice [42,43]. A generic signal transduction pathway starts with signal perception, followed by the generation of the second messengers )e.g., inositol phosphates and Reactive Oxygen Species (ROS)) and the transcription factors controlling the speci c sets of stress-regulated genes [44].…”

Section: Sensing and Signalingmentioning

confidence: 99%

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

Mansuri

Shobbar

Jelodar

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Salinity, as one of the main abiotic stresses, critically threatens growth and fertility of main food crops including rice in the world. To get insight into the molecular mechanisms by which tolerant genotypes responds to the salinity stress, we propose an integrative meta-analysis approach to find the key genes involved in salinity tolerance. Herein, a genome-wide meta-analysis, using microarray and RNA-seq data was conducted which resulted in the identification of differentially expressed genes (DEGs) under salinity stress at tolerant rice genotypes. DEGs were then confirmed by meta-QTL analysis and literature review. Results: A total of 3449 DEGs were detected in 46 meta-QTL positions, among which 1286, 86, 1729 and 348 DEGs were observed in root, shoot, seedling, and leaves tissues, respectively. Moreover, functional annotation of DEGs located in the meta-QTLs suggested some involved biological processes (e.g., ion transport, regulation of transcription, cell wall organization and modification as well as response to stress) and molecular function terms (e.g., transporter activity, transcription factor activity and oxidoreductase activity). Remarkably, 23 potential candidate genes were detected in Saltol and hotspot-regions overlying original QTLs for both yield components and ion homeostasis traits; among which, there were many unreported salinity-responsive genes. Some promising candidate genes were detected such as pectinesterase, peroxidase, transcription regulator, high-affinity potassium transporter, cell wall organization, protein serine/threonine phosphatase, and CBS domain cotaining protein. Conclusions: The obtained results indicated that, the salt tolerant genotypes use qualified mechanisms particularly in sensing and signalling of the salt stress, regulation of transcription, ionic homeostasis, and Reactive Oxygen Species (ROS) scavenging in response to the salt stress.

show abstract

Rapeseed calcineurin B-like protein CBL4, interacting with CBL-interacting protein kinase CIPK24, modulates salt tolerance in plants

Cited by 23 publications

References 25 publications

The Role of Na+ and K+ Transporters in Salt Stress Adaptation in Glycophytes

The Role of Na+ and K+ Transporters in Salt Stress Adaptation in Glycophytes

Transcriptome Analysis of Chrysanthemum lavandulifolium Response to Salt Stress and Overexpression a K+ Transport ClAKT Gene-enhanced Salt Tolerance in Transgenic Arabidopsis

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

Contact Info

Product

Resources

About