RAPD profiling in biological conservation: An application to estimating clonal variation in rare and endangered Iliamna in Virginia

Stewart, C. N.; Porter, Duncan M.

doi:10.1016/0006-3207(95)00023-w

Cited by 56 publications

(40 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The first 10 primers yielding reproducible patterns (primers A1, A2, A5, A8, A10, A11, A14, A15, A17 and A18) were selected for the RAPD analysis of all 80 sampled plants. The presence or absence of bands was scored for 74 band positions in the range of intermediate molecular weight (Stewart & Porter 1995).…”

Section: Methodsmentioning

confidence: 99%

Genetic structure of the annual weedSenecio vulgarisin relation to habitat type and population size

Müller‐Schärer

Fischer

2001

Molecular Ecology

View full text Add to dashboard Cite

Throughout the world, the highly selfing annual common groundsel, Senecio vulgaris (Asteraceae) is a common weed. Recently, it has also colonized ecological compensation areas in agro-ecosystems. We investigated the genetic structure of S. vulgaris using random amplified polymorphic DNA (RAPD) profiles of 80 plants from nine populations representing three habitat types in two regions in Switzerland. RAPD variation among regions (19.8%), among populations within regions (19.2%) and within populations (61.1%) was highly significant (ANOVA; P < 0.001). Gene flow estimated from the observed differentiation among populations (PhiST = 0.382) was low (assuming Wright's island model, Nem = 0.404). Genetic distances between pairs of populations were significantly correlated with geographical distances (Mantel test; r = 0.37, P < 0.03). Molecular variance obtained with AMOVA was lowest in the small populations in compensation areas (1.13), intermediate in vineyard populations (2.49), all located in northern Switzerland and highest in the larger vegetable field populations from western Switzerland (3.41; P < 0.05). Overall, there was a positive correlation of molecular variance and population size (P < 0.05), as expected under genetic drift. However, molecular variance was negatively correlated with population size among populations in ecological compensation areas, suggesting that selection was also important. We also applied triazine herbicide to leaves of three offspring of each of the 80 plants. Plants from populations of compensation areas showed higher mean levels and reduced variation in the resistance to triazine herbicide than plants from vineyards and vegetable fields. This suggests that compensation areas were colonized from adjacent corn fields, in which there has been selection for herbicide resistance. We discuss the implications of our results for the biological control of S. vulgaris.

show abstract

Section: Methodsmentioning

confidence: 99%

Genetic structure of the annual weedSenecio vulgarisin relation to habitat type and population size

Müller‐Schärer

Fischer

2001

Molecular Ecology

View full text Add to dashboard Cite

show abstract

“…Allozymes (reviewed in Ellstrand and Roose 1987; see also Parks and Werth 1993;Montalvo et al 1997;Mayes et al 1998;Pappert et al 2000), anonymous DNA markers (Hsiao and Rieseberg 1994;Stewart and Porter 1995;Stiller and Denton 1995;Steinger et al 1996;Olfelt et al 1998;Suzuki et al 1999;Corradini et al 2002) and a combination of both methods (Waycott 1995;Ayres and Ryan 1997;Esselman et al 1999;Ge and Sun 1999) have been effectively used in studies of clonal plant populations. Molecular methods are also valuable tools for identifying levels of genetic variation within and among plant populations, providing information that can be used to infer their reproductive systems and demographic histories.…”

Section: Introductionmentioning

confidence: 99%

The relative importance of sexual reproduction versus clonal spread in an aridland bunchgrass

et al. 2003

View full text Add to dashboard Cite

Festuca idahoensis (Idaho fescue) is a perennial caespitose grass, common in semi-arid rangelands of the Intermountain West. To determine how individuals are recruited into a population, we studied two long-term monitoring plots that were established in 1937 at the Northern Great Basin Experimental Range in southeastern Oregon. The plots measured 3.05x3.05 m, and were located approximately 30 m apart. One plot was ungrazed, the other was subject to moderate levels of cattle grazing. The number of F. idahoensis plants in both plots increased ten-fold between 1937 and 1996, but whether this was due primarily to reproduction by seed or clonal fragmentation was unknown. In 1996, we mapped and sampled 160 plants of F. idahoensis. We used dominant inter-simple sequence repeat (ISSR) markers and codominant allozyme markers in order to identify genetic individuals and measure genetic diversity. Both plots were characterized by high levels of genetic and clonal diversity. When information from ISSRs, allozymes and sample location were combined, 126 genets were recognized, each consisting of one to four samples (ramets). By measuring the diameter of clones surrounding plants that were present in 1937, we estimated that clonal spread occurred at a rate of approximately 3.7 cm per decade, and thus was of secondary importance in the maintenance and increase of F. idahoensis stands. Sexual reproduction, rather than clonal fragmentation, accounted for most of the recruitment of new plants into these plots. The grazed plot had fewer ramets, genotypes, and clones than the ungrazed plot, but the ramets were significantly larger. Levels of genetic diversity did not differ in the grazed and ungrazed plots, but there was some evidence for a small, but significant level of genetic differentiation between the two. The results also indicate that F. idahoensis has the potential to be a long-lived species with some individuals persisting in excess of 60 years. This study demonstrates how long-term monitoring can be supplemented by genetic analysis to obtain detailed information on the population dynamics of plants. In the case of this community dominant species, this provides essential information for understanding succession and developing management and restoration strategies.

show abstract

“…Different molecular methods can be efficiently used to study the gene pool of rare species. Nevertheless, for practical reasons, RAPD (random amplified polymorphic DNA; Williams et al, 1990) markers have been frequently used to determine the genetic variability of endangered, relict and/or endemic taxa (eg Stewart and Porter, 1995;Bauert et al, 1998;Liao and Hsiao, 1998;Las Heras et al, 1999;Maki and Horie, 1999). In this paper, the RAPD technique is applied to A. subbaeticum; more specifically, the following questions are addressed: (a) are levels of genetic variation present in this species similar to those reported in congeneric taxa?…”

Section: Introductionmentioning

confidence: 99%

Genetic variability in a narrow endemic snapdragon (Antirrhinum subbaeticum, Scrophulariaceae) using RAPD markers

et al. 2002

View full text Add to dashboard Cite

Antirrhinum subbaeticum is an endangered species inhabiting fragmented limestone cliffs. In the last 3 years, a drastic population decline has been observed in three of four known populations and the estimated number of surviving individuals is now close to 400. A RAPD study was conducted to evaluate the levels of genetic variation present in this species to improve conservation guidelines. Thity-nine polymorphic products identified 66.1% of the samples by unique RAPD multilocus profiles. A cluster analysis grouped the samples into two broad groups corresponding to northern or southern provenances. AMOVA analysis showed that only 17.7% of the genetic diversity was partitioned within populations. These results are in contrast to data available for

show abstract

RAPD profiling in biological conservation: An application to estimating clonal variation in rare and endangered Iliamna in Virginia

Cited by 56 publications

References 14 publications

Genetic structure of the annual weedSenecio vulgarisin relation to habitat type and population size

Genetic structure of the annual weedSenecio vulgarisin relation to habitat type and population size

The relative importance of sexual reproduction versus clonal spread in an aridland bunchgrass

Genetic variability in a narrow endemic snapdragon (Antirrhinum subbaeticum, Scrophulariaceae) using RAPD markers

Contact Info

Product

Resources

About