RAPD Profile Analysis of Single and Multigrain Aman Rice (Oryza sativa L.) Varieties Available in Bangladesh

Abstract

Genetic variation in 14 local Aman rice varieties of Bangladesh including single grain and multigrain rice was investigated at the DNA level by RAPD molecular typing. Fifteen random primers were initially screened against DNA from five individuals that generated highly reproducible RAPD fragments, which were then subjected to further population analysis. A total 191 RAPD fragments were generated with these primers and 153 (80.1%) were polymorphic, which indicated high level of polymorphism existed within these populations. The size of amplified fragments ranged between 450 and 6000 bp. Pair-wise distance estimated the range of 0.03 to 0.59 between single and multigrain Aman rice varieties. Results illustrated the potential of RAPD markers to distinguish improved varieties and grain specificity at DNA level.

Effects of potassium on panicle structure and spikelet morphology of a double grained rice cultivar

“…1). Mitra et al (2017) reported the lowest genetic distance between two multi-grain cultivars, Biram sundari and Double rice, which indicates close relations within multi-grain varieties. Guarino (2012) found that the clustered spikelets may be borne directly on the long primary branches of the panicle or on short secondary branches in varying proportions.…”

“…2.5 -3.0 t/ha and suitable for cultivation in both Aman and Boro seasons (Anon. 2012, Mitra et al 2017. It can be used as a potential genetic resource for further breeding programme to ensure the food security in future (Ren et al 2018).…”

“…It can be used as a potential genetic resource for further breeding programme to ensure the food security in future (Ren et al 2018). Although the genetic/molecular basis of multiple florets(/grains) spikelets development in rice has been described since long ago (Parthasarathy 1936, Morinaga and Tajiri 1941, Kasahara 1944, Li et al 2007, Mitra et al 2017, Zhang et al 2017, Ren et al 2019, Zheng et al 2019, reports on morphology and/or developmental anatomy are scanty (Nasiruddin 1963, Shimizu and Kuno 1966, Sarwar and Chanda 2018. Moreover, multiple-seeded rice sometime identified as a separate species of Oryza, O. plena (Prain) Chowdhury (Chowdhury 1949).…”

Performance of double grained rice cultivar under different plant spacing

“…Biram sundari, a double grained rice cultivar with a grain yield of 2.5 -3.0 t ha -1 was reported from Birampur Upazila of Dinajpur district in Bangladesh, previously (Anon., 2012; Mitra et al, 2017). The multiple pistils are responsible for multiple grains within a single rice spikelet which is a very rare and unique feature in rice morphology (Sarwar and Chanda, 2018).…”