RAPD PCR discloses high genetic heterogeneity among Vibrio parahaemolyticus from various environments along the southwest coast of India

Silvester, Reshma; Alexander, Deborah; Sudha, S.; Hatha, Mohamed

doi:10.1007/s13213-015-1164-4

Cited by 4 publications

(1 citation statement)

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“…RAPD-PCR is a common method to perform genotyping among Vibrio spp. as this procedure is known to be able to reveal a high level of DNA diversity within common bacterial isolates, and thus is useful in discriminating the isolates (Silvester, Alexander, Santha, & Hatha, 2016). Vincent et al (2015) conducted a study to assess the rapid molecular identification and characterization of 45 Vibrio parahaemolyticus isolates from 15 samples of 3 different types of fish.…”

Section: Randomly Amplified Polymorphic Dnas (Rapd)mentioning

confidence: 99%

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Mısır¹

2018

GenAqua

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Global seafood consumption has significantly increased due to the health concerns of populations. One of the basic requirements to meet the increasing demand for seafood is security and safety. To meet these requirements, detection and identification of seafood pathogens are of importance. For this purpose, besides conventional methods a variety of rapid, sensitive and reliable techniques have been developed. In general, rapid detection methods are generally time-efficient, sensitive, specific and labor-saving. This review emphasizes on the principles and applications of recent rapid methods for the detection of seafood pathogens; including polymerase chain reaction (PCR), multiplex PCR, Random Amplified Polymorphic DNA (RAPD), Real Time Polymerase Chain Reaction or Quantitative Polymerase Chain Reaction (qPCR), Denaturation Gradient Gel Electrophoresis (DGGE), loop-mediated isothermal amplification (LAMP).

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Section: Randomly Amplified Polymorphic Dnas (Rapd)mentioning

confidence: 99%