RAPD markers linked to a gene for resistance to pine needle gall midge in Japanese black pine (Pinus thunbergii)

Kondo, Tohru; Terada, Kinya; Hayashi, Eiji; Kuramoto, Noritsugu; Okamura, Masanori; Kawasaki, Hideki

doi:10.1007/s001220050051

Cited by 29 publications

(23 citation statements)

References 12 publications

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“…It is known that AFLP markers reveal a larger number of polymorphic bands than other techniques like RAPDs. In tree species the average number of fragments per primer combination ranged from six (Salix, Tsarouhas et al 2002) to 26 (Larix, Arcade et al 2000), while for RAPD polymorphic fragments per assay ranged from 1.3 (Pinus thunbergii, Kondo et al 2001) to 4.8 (Pinus pinaster, Costa et al 2000). In our study the average number of fragments per primer combination was 7.4 and 1.7 for AFLPs and RAPDs, respectively.…”

Section: Qtl Analysismentioning

confidence: 89%

A RAPD, AFLP and SSR linkage map, and QTL analysis in European beech (Fagus sylvatica L.)

et al. 2003

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The genetic linkage map of European beech ( Fagus sylvatica L.) that we report here is the first to our knowledge. Based on a total of 312 markers (28 RAPDs, 274 AFLPs, 10 SSRs) scored in 143 individuals from a F(1) full-sib family. Two maps (one for each parent) were constructed according to a "two-way pseudo-testcross" mapping strategy. In the male map 119 markers could be clustered in 11 major groups (971 cM), while in the female map 132 markers were distributed in 12 major linkage groups (844 cM). In addition, four and one minor linkage groups (doublets and triplets) were obtained for the male and female map respectively. The two maps cover about 82% and 78% of the genome. Based on the position of 15 AFLP and 2 SSR loci segregating in both parents, seven homologous linkage groups could be identified. In the same pedigree we investigated the association with genetic markers of several quantitative traits: leaf area, leaf number and shape in 2 different years, specific leaf area, leaf carbon-isotope discrimination and tree height. A composite interval-mapping approach was used to estimate the number of QTLs, the amount of variation explained by each of them, and their position on the genetic linkage maps. Eight QTLs associated with leaf traits were found that explained between 15% and 35% of the trait variation, five on the female map and three on the male map.

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Section: Qtl Analysismentioning

confidence: 89%

A RAPD, AFLP and SSR linkage map, and QTL analysis in European beech (Fagus sylvatica L.)

et al. 2003

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“…A study of the Pinus genome is underway as part of ongoing efforts to improve the growth and quality of wood and the resistance of trees to disease and harmful insects. Consequently, gene linkage maps have been constructed in several species (Remington et al 1999;Sewell et al 1999;Costa et al 2000;Kondo et al 2000;Kubisiak et al 2000;Li and Yeh 2001). However, elucidation of the connections between linkage groups and individual chromosomes in different species has been hampered by problems with the identification of chromosomes using cytological methods.…”

Section: Introductionmentioning

confidence: 99%

Chromosome identification and comparative karyotypic analyses of four Pinus species

et al. 2002

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Chromosomal landmarks in four Pinus species: P. densiflora, P. thunbergii, P. sylvestris, and P. nigra were identified by fluorescence in situ hybridization (FISH) using hapten- or fluorochrome-labeled probes for the plant telomere repeat, centromeric repeat ( PCSR), and rDNA. FISH landmarks were located at the interstitial and proximal regions of chromosomes and allowed us to identify nearly all of the homologous chromosomes in each species. A comparative analysis of the FISH karyotypes among the four species showed that the interstitial FISH signals obtained by hybridization with the telomere and rDNA sequences were stable and could be used to identify homologous chromosomes among species. The identification of homologous chromosomes among species facilitated a detailed comparative karyotype analysis. The results suggest that the degree of chromosomal differentiation among the four Pinus species is very low and that the proximal regions vary in their DNA sequences. The similarities and differences among FISH karyotypes are discussed in relation to phylogeny.

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“…Particularly, Bundock et al (2000) verified the usefulness of the RAPD markers in association with microsatellites for the construction of E. globulus maps. This approach to the use of different markers was also proposed by Kondo et al (2000) who mapped a resistance gene to pine needle gall midge based on RAPD markers, understanding the need for more closely linked markers; localized mapping could equally contribute to the saturation of the linkage map close to the gene of interest.…”

Section: Resultsmentioning

confidence: 99%

“…Molecular markers at the DNA level have allowed the construction of genetic linkage maps of forest species such as Eucalyptus (Grattapaglia and Sederoff 1994, Byrne et al 1995, Bundock et al 2000, Populus (Liu andFurnier 1993, Bradshaw et al 1994) and conifers , Sewell et al 2000, Kondo et al 2000, Li and Yeh 2001 as well as the localization of genomic regions that control economically important traits in these species (Groover et al 1994, Benet et al 1995, Bradshaw et al 1995, Grattapaglia et al 1995, Plomion 1995, Sewell et al 2000. 1International Paper do Brasil, Rodovia SP340, km171, C. P. 10, 13.840-970, Mogi Guaçu, SP, Brazil 2Laboratório de Bioquímica e de Biologia Molecular, UNESP, Via de Acesso Prof. Paulo Donato Castelane, Km 05, 14.884-900, Jaboticabal, SP, Brasil 3Cenagen, Embrapa, Av.…”

Section: Introductionmentioning

confidence: 99%

Localized mapping of RAPD markers in Eucalyptus grandis

Campinhos

Laia

Grattapaglia

et al. 2005

CBAB

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RAPD markers linked to a gene for resistance to pine needle gall midge in Japanese black pine (Pinus thunbergii)

Cited by 29 publications

References 12 publications

A RAPD, AFLP and SSR linkage map, and QTL analysis in European beech (Fagus sylvatica L.)

A RAPD, AFLP and SSR linkage map, and QTL analysis in European beech (Fagus sylvatica L.)

Chromosome identification and comparative karyotypic analyses of four Pinus species

Localized mapping of RAPD markers in Eucalyptus grandis

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