RAPD markers and black pine (Pinus nigra Arnold) intraspecies taxonomy - Evidence from the study of nine populations

Abstract

Although intraspecies researches within the black pine (Pinus nigra Arnold) have a long tradition, the intraspecies taxonomy, classification and chorology are still unclear. Among the numerous reasons that have caused this situation the most important are: the absence of a study that would completely cover the whole range of this species, the impossibility of connection of results of the existing detailed studies of certain areas, and the high variability of traits which have been used so far. Since the characteristics of the molecular systematic techniques could make possible the research free of the mentioned shortages, the intention of this study was to determine the relationships among nine populations of black pine using the random amplified polymorphic DNA (RAPD). The obtained results were compared to the recent results of the morphological and anatomical analysis of the leaves of the same populations. The RAPD results clearly divided the Croatian populations from populations of Austria (subsp. nigra) and Turkey (subsp. pallasiana), while among Croatian populations, as in previous study, the existence of several groups (subsp. illyrica, subsp. dalmatica and transitional population between them) was noticed. It is assumed that the optimisations conducted in this study will finally make possible estimating the relationships on the level of the whole range of the black pine and the classification based on molecular traits that are probably less dependent on environmental influences than it has been the case with the characteristics mostly used so far.

Geographically Related Variation in Epicuticular Wax Traits of Pinus nigra Populations from Southern Carpathians and Central Balkans – Taxonomic Considerations

“…It is interesting that Liber et al . , using the random amplified polymorphic DNA (RAPD), also pointed to the existence of several P. nigr a groups on the territory of western Balkans (subsp. illyrica , subsp.…”

Different karyotype patterns among allopatric Pinus nigra (Pinaceae) populations revealed by molecular cytogenetics

“…Biochemical and molecular markers recognise the P. nigra subspecies, but with low taxonomical resolution (Nikolić & Tucić 1983; Aguinagalde et al. 1997; Liber et al. 2003).…”

“…Biochemical and molecular markers recognise the P. nigra subspecies, but with low taxonomical resolution (Nikolić & Tucić 1983;Aguinagalde et al 1997;Liber et al 2003). The recent cpSSR study of Raffi & Dodd (2007) showed high within-and low among-population differences across the western range of European black pine, differentiating it into six geographic regions of molecular variation.…”

First report of Eulachnus tuberculostemmatus (Theobald, 1915) on Pinus nigra subsp. mauretanica stands in Algeria

“…) is the southernmost subspecies of black pine, and is endemic along the Western Moroccan Rif and the Algerian Tell Atlas (Liber et al, 2003;Qu ezel & M edail, 2003). mauretanica (Maire et Peyerimh.…”

“…mauretanica (Maire et Peyerimh. ) is the southernmost subspecies of black pine, and is endemic along the Western Moroccan Rif and the Algerian Tell Atlas (Liber et al, 2003;Qu ezel & M edail, 2003). Currently, an extremely limited number of plants still persist within the natural range (M edail & Diadema, 2009), giving this taxon an outstanding ecological importance (Arbez & Millier, 1971;Seigue, 1985).…”