RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagated Dendrobium fimbriatum Lindl. var. oculatum Hk. f.- an important endangered orchid

Tikendra, Leimapokpam; Potshangbam, Angamba Meetei; Dey, Abhijit; Devi, Tongbram Roshini; Sahoo, Manas Ranjan; Nongdam, Potshangbam

doi:10.1007/s12298-021-00939-x

Cited by 47 publications

(17 citation statements)

References 56 publications

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“…One of these is the random amplified polymorphic DNA (RAPD) PCR technique, which is a rapid, inexpensive, and simple method for detecting genetic differences as it does not require any previous information about the plant genome [13]. RAPD-PCR was used to determine the genetic stability in micropropagated plants of Prunus salicina [14], Echinacea purpurea [15], Dendrobium fimbriatum [16], and Rhynchostylis retusa [17]. A more specific technique than RAPD is the inter-simple sequence repeats (ISSR) PCR technique.…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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Lycium schweinfurthii is a Mediterranean wild shrub rich in plant secondary metabolites. In vitro propagation of this plant may support the production of valuable dietary supplements for humanity, introduction of it to the world market, and opportunities for further studies. The presented study aimed to introduce an efficient and reproducible protocol for in vitro micropropagation of L. schweinfurthii and assess the genetic stability of micropropagated plants (MiPs) as well as to estimate phenolic, flavonoid, ferulic acid contents, and the antioxidant activity in leaves of micropropagated plants. Two DNA-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), and one biochemical technique, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), were used to assess the genetic stability in MiPs. Spectrophotometric analysis was performed to estimate total phenolic and flavonoid contents and antioxidant activity of MiPs leaves, while ferulic acid content was estimated using high-performance thin-layer chromatography (HPTLC). Sufficient shoot proliferation was achieved at MS (Murashige and Skoog) medium supplemented with 0.4 mg L−1 kinetin and rooted successfully on half-strength MS medium fortified with 0.4 mg L−1 Indole-3-butyric acid (IBA). The Jaccard’s similarity coefficients detected in MiPs reached 52%, 55%, and 82% in the RAPD, ISSR, and SDS-PAGE analyses, respectively. In the dried leaves of MiPs, the phenolic, flavonoid, and ferulic acid contents of 11.53 mg gallic acid equivalent, 12.99 mg catechin equivalent, and 45.52 mg were estimated per gram, respectively. However, an IC50 of 0.43, and 1.99 mg mL−1 of MiP dried leaves’ methanolic extract was required to scavenge half of the DPPH, and ABTS free radicals, respectively. The study presented a successful protocol for in vitro propagation of a valued promising plant source of phenolic compounds.

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Section: Introductionmentioning

confidence: 99%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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“…The average sizes of the bands were ranged from 200-1500 bp ( g 3a & 3b). RAPD analysis showed no polymorphism in several plant species such as Prunus dulcis (Martins et al 2004), Phoenix dactylifera (Saker et al 2006), Jatropha curcas (Sharma et al 2011), Thalictrum foliolosum (Mishra et al 2020), Solanum viarum (Pandey et al 2020), Solanum erianthum (Sarkar and Banarjee 2020), Rhododendron mucronulatum (Novikova et al 2020) and Dendrobium mbriatum (Tikendra et al 2021). In ISSR markers, the banding patterns were ranged from 2 (UBC-804) to 8 (UBC-812) with an average of 5 bands per primer and size range of 200-1000 bp (Fig 3c & 3d).…”

Section: Main Textmentioning

confidence: 99%

“…In ISSR markers, the banding patterns were ranged from 2 (UBC-804) to 8 (UBC-812) with an average of 5 bands per primer and size range of 200-1000 bp (Fig 3c & 3d). In modern years, ISSR markers deemed as a reliable marker to assess the genetic stability of plantlets for instance Haloxylon persicum (Kurup et al 2018), Eucalyptus nitens (Ayala et al 2019), Albizia lebbeck (Saeed et al 2019), Sapium sebiferum (Hou et al 2020), Cicer arietinum (Sadhu et al 2020), Pterocarpus marsupium (Ahmad et al 2020), Ficus carica (Abdolinejad et al 2020), Rhododendron mucronulatum (Novikova et al 2020) and Dendrobium mbriatum (Tikendra et al 2021). As a result, the monomorphic banding pattern produced by RAPD and ISSR markers con rms the genetic stability among the regenerants and the mother plants.…”

Section: Main Textmentioning

confidence: 99%

Efficient in vitro plant regeneration from leaf-derived callus and genetic fidelity assessment of an endemic medicinal plant Ranunculus wallichianus Wight & Arnn by using RAPD and ISSR markers

Srinivasan

Raja

Tamilvanan

2021

Plant Cell Tiss Organ Cult

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Ranunculus wallichianus is a medicinally important plant and an endemic species to Western Ghats of South India. An e cient and reliable indirect regeneration protocol system for R. wallichianus was developed from leaf explants in the present investigation. Leaf explants were cultured on both fullstrength and half-strength MS (Murashige & Skoog) medium supplemented with different concentrations (1.0 mg L − 1 to 3.0 mg L − 1 ) of 2,4-D and NAA. Among the different concentrations tested, the highest percentage of yellowish green compact nodular callus formation was observed on half-strength MS medium with 2.0 mg L − 1 of 2, 4-D. Then, the in vitro raised organogenic callus was cultured on half strength MS medium containing various concentrations (1.0 mg L − 1 to 3.0 mg L − 1 ) of BA, KIN and TDZ with 0.5 mg L − 1 NAA and 10% CW for in vitro shoot regeneration. The highest percentage of regeneration response (97%) and maximum number of shoots formation (11.1 ± 0.13 shoots/culture with 9.2 ± 0.35 cm mean shoot length) were obtained from MS medium containing 2.5 mg L − 1 BA with 0.5 mg L − 1 NAA and 10% CW. The well elongated in vitro raised shoots were rooted in half strength MS medium with 2.5 mg L − 1 IBA + 250 mg L − 1 activated charcoal shows high frequency of root formation. The well rooted plantlets were successfully hardened and acclimatized with the survival rate of 94%. Clonal delity of in vitro raised plantlets was assessed by using DNA based RAPD and ISSR molecular markers. The total of 56 and 47 monomorphic bands were obtained from RAPD and ISSR markers respectively. This present in vitro propagation protocol system could be an effective for the conservation of R. wallichianus with their genetic purity and its further investigations.

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“…Then, only 1.52%, 1.19% and 3.97% polymorphisms were observed with RAPD, ISSR and SCoT markers respectively when the plants were grown in hormone enriched medium. This considerably high genetic stability indicated a successful in vitro propagation of the orchid species (Tikendra et al, 2021).…”

Section: Resultsmentioning

confidence: 97%

RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

Susanto

Romadhoni

Dwiati

2022

molekul

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Rhynchostylis gigantea (Lindl.) Ridl. is an orchid species spread over Southeast Asia countries. This species is very popular among ornamental plant collectors, especially due to its densely pack inflorescences. Hence, it is commercially found in many ornamental plant nursuries, such as Puspa Nirmala Orchids Banyumas, Central Java. Further development of the species should be supported by scientific data, particularly regarding the genetic variation. One of the molecular markers commonly used to study genetic variation is Random Amplified Polymorphic DNA (RAPD). This study aims to assess genetic variation of R. gigantea cultivars of Puspa Nirmala Orchids Banyumas collection by RAPD profiles. Genomic DNAs were extracted from leaf samples of eight R. gigantea individuals, while RAPD markers were amplified using five random primers (OPA-15, OPK-16, OPP-15, OPP-08 and OPO-08). Descriptive analysis was employed on the data obtained. It was revealed that all of the primers resulted in a 100% monomorphism. This indicates an extremely low genetic variation among R. gigantea population of Puspa Nirmala Orchids collection, which is probably due to the same origin from a selected hybrid of the same crosses.

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RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagated Dendrobium fimbriatum Lindl. var. oculatum Hk. f.- an important endangered orchid

Cited by 47 publications

References 56 publications

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Efficient in vitro plant regeneration from leaf-derived callus and genetic fidelity assessment of an endemic medicinal plant Ranunculus wallichianus Wight & Arnn by using RAPD and ISSR markers

RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

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