RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas

Guerra, J. C.; Issa, Marcia Regina Cavichio; Carneiro, Francisco Estevão; Strapazzon, Roger; Moretto, Geraldo

doi:10.4238/vol9-1gmr696

Cited by 22 publications

(22 citation statements)

References 10 publications

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“…However, with Africanized honeybee queens, Souza et al (2013) found values of 180 to 200 mg, while Tarpy et al (2011; reported values of 206.6 and 218.7 mg for European honeybee queens. Even with lower weight of European honeybee queens, they showed a more hygienic behavior, once their infestation index by Varroa destructor is lower compared with the European queen (Guerra Júnior et al, 2010).…”

Section: Methodsmentioning

confidence: 92%

<b>Morphometric measurements of Africanized honeybee queens kept in an incubator or in queen banking

Metorima

Costa-Maia

Halak

et al. 2015

Acta Sci. Anim. Sci.

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ABSTRACT. This study aimed to estimate live weight, the length and the width of wing and abdomen and the length, the width and the height of thorax in newly emerged Africanized honeybee queens up to 192 hours and their influence on final quality regarding appropriate storage techniques in queen banking or incubator and to verify the possibility of using these parameters as selection criteria in honeybee breeding programs. Queen honeybees were produced in summer, autumn and winter. The queen production method used throughout the experiments was adapted from Doolittle, and 68 the queens were weighed at emergence. Data were analyzed in R software by regression analysis of queen weight at emergence according to life time after emergence, in two types of storage. The weight of queens emerged in queen banking and incubator was significantly different (p < 0.05), with mean values of 165.61 and 157.04 mg, respectively. Queens kept in an incubator and in queen banking showed no significant differences (p > 0.05) in wing length, abdomen length and thorax height. The weight of queen at emergence can be used as a selection criterion in honeybee breeding programs.Keywords: queen size, emergence, honeybee breeding, queen production.Medidas morfométricas de rainhas africanizadas mantidas em estufa ou colmeia banco de rainhas RESUMO. O objetivo do trabalho foi estimar peso vivo, comprimento e largura de asa e abdome e comprimento, largura e altura do tórax de rainhas Apis mellifera africanizadas recém-emergidas até 192h e sua influência na qualidade final com relação às técnicas apropriadas de armazenamento em banco de rainhas ou estufa e verificar se estes parâmetros podem ser utilizados como critério de seleção. Foram produzidas nas seguintes estações do ano: verão, outono e inverno. O método utilizado durante todo o experimento foi o adaptado de Doolitlle para a produção de rainhas e 68 rainhas foram pesadas à emersão. Os dados foram analisados no software R pela análise de regressão do peso da rainha à emergência em função do tempo de vida após a emersão, nos dois tipos de alojamento. Para peso, os efeitos da colmeia banco de rainhas e estufa foram significativos (p < 0,05), médias de 165,61 e 157,04 mg, respectivamente. As rainhas mantidas em estufa e colmeia banco de rainhas não apresentaram diferenças em relação ao comprimento da asa, ao comprimento do abdome e à altura do tórax. O peso de rainhas à emergência mantidas em colmeia banco de rainhas foi maior que o peso das rainhas mantidas em estufa. O peso das rainhas à emergência pode ser utilizado como critério de seleção.Palavras-chave: tamanho de rainhas, emersão, melhoramento de abelhas, produção de rainhas.

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Section: Methodsmentioning

confidence: 92%

<b>Morphometric measurements of Africanized honeybee queens kept in an incubator or in queen banking

Metorima

Costa-Maia

Halak

et al. 2015

Acta Sci. Anim. Sci.

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“…Among the methodologies available, the RAPD-PCR technique has been amply utilized in different organisms, due to its aforementioned characteristics, with results considered important for this objective (Garcia et al, 1998;Iqbal et al, 2007;Camargo Jr. et al, 2007;Roratto et al, 2008;Guerra Jr. et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR

Guerra

Lima²,

Taddei³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We analyzed three species of this genus (M. jelskii, M. amazonicum and M. brasiliense) using RAPD-PCR to assess their genetic variability, genetic structure and the phylogenetic relationship between them and to look for molecular markers that enable separation of M. jelskii and M. amazonicum, which are closely related syntopic species. Ten different random decamer primers were used for DNA amplification, yielding 182 fragments. Three of these fragments were monomorphic and exclusive to M. amazonicum or M. jelskii and can be used as specific molecular markers to identify and separate these two species. Similarity indices and a phylogenetic tree showed that M. amazonicum and M. jelskii are closest to each other, while M. brasiliense was the most differentiated species among them; this may be attributed to the different habitat conditions to which these species have been submitted. This information will be useful for further studies on these important crustacean species.

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“…It thereafter spread on A. mellifera into Europe in the 1960s (Crane 1978), and then around the world, arriving in North America in 1987(De Guzman et al 1997Oldroyd 1999;Sammataro et al 2000). Latest studies detected the presence of the Korean haplotype in Argentina (Anderson and Trueman 2000;Guerra et al 2010), México, Cuba, Venezuela, Cuba, Chile, Uruguay and Colombia (Guerra et al 2010). Varroa destructor and specially the Korean haplotype, represents the major honey bee pest infesting and destroying millions of commercial beehives around the world (Beetsma 1994;Sammataro et al 2000).…”

Section: Abstract Varroa Destructor á Dna á Haplotypes á Argentinamentioning

confidence: 95%

Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina

Maggi

Medici

Quintana³

et al. 2012

Exp Appl Acarol

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Although mitochondrial DNA mapping of Varroa destructor revealed the presence of several haplotypes, only two of them (Korean and Japanese haplotypes) were capable to infest Apis mellifera populations. Even though the Korean haplotype is the only one that has been reported in Argentina, these conclusions were based on mites sampled in apiaries from a specific geographical place (Buenos Aires province). To study mites from several sites of Argentina could reveal the presence of the Japanese genotype, especially considering sites near to Brazil, where Japanese haplotype was already detected. The aim of this work was to study the genetic structure of V. destructor populations from apiaries located in various provinces of Argentina, in order to determine the presence of different haplotypes. The study was carried out between January 2006 and December 2009. Phoretic adult Varroa mites were collected from honey bee workers sampled from colonies of A. mellifera located in Entre Ríos, Buenos Aires, Corrientes, Río Negro, Santa Cruz and Neuquén provinces. Twenty female mites from each sampling site were used to carry out the genetic analysis. For DNA extraction a nondestructive method was used. DNA sequences were compared to Korean haplotype (AF106899) and Japanese haplotype (AF106897). All DNA sequences obtained from mite populations sampled in Argentina, share 98% of similitude with Korean Haplotype (AF106899). Taking into account these results, we are able to conclude that Korean haplotype is cosmopolite in Argentina.

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RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas

Cited by 22 publications

References 10 publications

<b>Morphometric measurements of Africanized honeybee queens kept in an incubator or in queen banking

<b>Morphometric measurements of Africanized honeybee queens kept in an incubator or in queen banking

Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR

Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina

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