RAPD based genetic diversity among different isolates of Fusarium oxysporum f. sp. lycopersici and their comparative biocontrol

Mishra, Kundan K.; Kumar, Ashish; Pandey, Koshlendra Kumar

doi:10.1007/s11274-009-0273-2

Cited by 13 publications

(8 citation statements)

References 9 publications

(9 reference statements)

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“…P values are expressed as mg of P 2 O 5 per kg of soil, and the conversion factor for P is 0.44. There were significant differences among plantations for 6 . Unfortunately, critical value of P is not available for asparagus.…”

Section: Soil Propertiesmentioning

confidence: 93%

Identification of phosphate-solubilizing fungi from the asparagus rhizosphere as antagonists of the root and crown rot pathogen Fusarium oxysporum

Ruangsanka¹

2014

ScienceAsia

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Identification of phosphate solubilizing fungi in the root zone of asparagus that can inhibit the growth of root and crown rot pathogen (Fusarium oxysporum) is important for the development of bio-fertilizer and bio-control strategies for organic production of asparagus. This study aimed to identify fungi with the ability to solubilize phosphate and inhibit the growth of F. oxysporum in vitro. Twenty five soil samples were collected from the asparagus rhizosphere in the planting areas of Dan Ma Kham Tea district, Kanchanaburi province, Thailand. A total of 59 fungi that could solubilize phosphate in Pikovskaya medium were isolated from 25 soil samples. Fourteen out of 59 fungal isolates were further screened and four isolates were selected. These isolates were tested for growth inhibition of F. oxysporum. One isolate was identified as Penicillium oxalicum and had the highest phosphate solubilizing ability (556 mg P/l). Another isolate was identified as Aspergillus niger and had the highest pathogen inhibition percentage (64%). These isolates are promising for the development of bio-fertilizer and bio-control of F. oxysporum for organic production of asparagus.

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Section: Soil Propertiesmentioning

confidence: 93%

Identification of phosphate-solubilizing fungi from the asparagus rhizosphere as antagonists of the root and crown rot pathogen Fusarium oxysporum

Ruangsanka¹

2014

ScienceAsia

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“…causing wilt disease and found the DNA band size ranged from 200 bp to 5090 bp and 300bp to 2kb respectively. Mishra et al (2010) also reported up to 20% genetic diversity by RAPD profiles of ten Fusarium oxysporum f. sp. lycopersici strains.…”

Section: Genetic Variability Of Fusarium Isolatesmentioning

confidence: 89%

“…A set of seven random primers used by Bahmani et al (2012) revealed a total of 36 alleles in 24 fumonisic F. verticillioides. Gupta (2012) and Mishra et al (2010) tested 30 and 25 RAPD primers respectively with the genome of Fusarium spp. causing wilt disease and found the DNA band size ranged from 200 bp to 5090 bp and 300bp to 2kb respectively.…”

Section: Genetic Variability Of Fusarium Isolatesmentioning

confidence: 99%

“…Many workers have extensively studied PCR Based genetic variability or polymorphism. The Randomly Amplified Polymorphic DNA (RAPD) assay in which DNA segments are amplified by arbitrary primers, has been used for genetic characterisation of Fusarium species and other mycotoxigenic fungi (Nelson et al, 1997;Mostafa et al, 2002;Belabid et al, 2004;Sharma et al, 2006;Lourenço et al, 2007;Gupta et al, 2009;Mishra et al, 2010;Mohammadian et al, 2011;Gupta, 2012;Bahmani et al, 2012;Bonde et al, 2013;Ebadi et al, 2013). RAPD-genetic characterisation of Fusarium species from animal feed have already been carried out based on fumonisin production.…”

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confidence: 99%

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Molecular detection and genetic characterisation of fumonisin producingfusariumisolates from rice cultivars

Nayak¹,

Dhua²,

Samanta³

et al. 2014

Intern. Jour. of Agricul., Environ. and Biotech.

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Fusarium species mainly produce fumonisins group of mycotoxins which are classified as Group 2B human carcinogen by International Agency for Research on Cancer (IARC). In poor storage conditions, Fusarium species producing fumonisins can infect rice or paddy (Oryza sativa L.) which is the highest produced and consumed staple food in India. A rapid molecular method using primer Fum5F and Fum6R detected 85% fumonisin producers among 28 Fusarium isolates from Indian rice cultivars. Genetic variability of the isolates was studied by PCR based RAPD assay using 13 random primers. A total of 169 polymorphic bands were obtained by 13 markers with an average polymorphism information content (PIC) of 0.665 and overall polymorphism of 88%. Primer 3B showed a polymorphism of 96% with PIC value of 0.66 and it amplified 26 scorable fragments hence may be useful for the analysis of genetic variation among Fusarium isolates. Four strains (F47, F90, F92 and F96) in which fum gene wasn't amplified by Fum5F and Fum6R and supposed to be non producer of fumonisin have been consistently placed in one separate group by RAPD primers. Genetic variation of toxic Fusarium in rice from India is less studied. RAPD proved to be a suitable tool for depicting Polymorphism among the isolates. The high genetic variability among the Fusarium isolates used in the current study is a matter of concern considering the importance of Rice in India. Highlights• Fumonisins are Group 2B human carcinogen produced by Fusarium species.• Fum5F and Fum6R primer pair detected fumonisin producers.• RAPD primers deciphered 88% polymorphism among 26 Fusarium isolates.

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“…The use of resistant varieties is the most economical and effective way to manage the disease. However, new races of pathogen have been emerged that overcome resistance in currently growing tomato cultivars (Mishra et al, 2010). Therefore, knowledge of the genetic variation within and among populations is an important component to understand the population biology of F. oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 99%

Computational Mining and Genome Wide Distribution of Microsatellite in Fusarium oxysporum f. sp. lycopersici

et al. 2012

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Simple sequence repeat (SSR) is currently the most preferred molecular marker system owing to their highly desirable properties viz., abundance, hyper-variability, and suitability for high-throughput analysis. Hence, in present study an attempt was made to mine and analyze microsatellite dynamics in whole genome of Fusarium oxysporum f. sp. lycopersici. The distribution pattern of different SSR motifs provides the evidence of greater accumulation of tetra-nucleotide (3837) repeats followed by tri-nucleotide (3367) repeats. Maximum frequency distribution in coding region was shown by mono-nucleotide SSR motifs (34.8%), where as minimum frequency is observed for penta-nucleotide SSR (0.87%). Highest relative abundance (1023 SSR/Mb) and density of SSRs (114.46 bp/Mb) were observed on chromosome 1, while least density of SSR motifs was recorded on chromosome 11 (7.40 bp/Mb) and 12 (7.41 bp/Mb), respectively. Maximum trinucleotide (34.24%) motifs code for glutamic acid (GAA) while GT/CT were the most frequent repeat of dinucleotide SSRs. Most common and highly repeated SSR motifs were identified as (A)64, (T)48, (GT)24, (GAA)31, (TTTC)24, (TTTCT)28 and (AACCAG)27. Overall, the generated information may serve as baseline information for developing SSR markers that could find applications in genomic analysis of F. oxysporum f. sp. lycopersici for better understanding of evolution, diversity analysis, population genetics, race identification and acquisition of new virulence.

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RAPD based genetic diversity among different isolates of Fusarium oxysporum f. sp. lycopersici and their comparative biocontrol

Cited by 13 publications

References 9 publications

Identification of phosphate-solubilizing fungi from the asparagus rhizosphere as antagonists of the root and crown rot pathogen Fusarium oxysporum

Identification of phosphate-solubilizing fungi from the asparagus rhizosphere as antagonists of the root and crown rot pathogen Fusarium oxysporum

Molecular detection and genetic characterisation of fumonisin producingfusariumisolates from rice cultivars

Computational Mining and Genome Wide Distribution of Microsatellite in Fusarium oxysporum f. sp. lycopersici

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