RAPD and VNTR analyses demonstrate genotypic heterogeneity of Mycoplasma hyopneumoniae isolates from pigs housed in a region with high pig density

Nathues, Heiko; Beilage, Elisabeth große; Kreienbrock, Lothar; Rosengarten, Renate; Spergser, Joachim

doi:10.1016/j.vetmic.2011.05.029

Cited by 31 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results agree with those described previously for isolates obtained from pneumonic lungs (NATHUES et al, 2011;CHARLEBOIS et al, 2014). To our knowledge, this is the first study that shows MLVA genotypes of M. hyopneumoniae present in swine lungs without pneumonic lesions in regard to the MLVA genotypes present in pneumonic lungs.…”

Section: Discussionsupporting

confidence: 92%

“…Genetic heterogeneity between M. hyopneumoniae strains recovered from clinical samples of different or the same farms has been shown using different molecular methodologies (CALUS et al, 2007;VICCA et al, 2003;MAYOR et al, 2007;NATHUES et al, 2011;STAKENBORG et al, 2005;VRANCKX et al, 2011). The multiple loci variable number of tandem repeats (VNTR) analysis (MLVA) (VRANCKX et al, 2011), overcome the limitations of other typing techniques like random amplified polymorphic DNA (RAPD) (ARTIUSHIN; , amplified fragment length polymorphism (AFLP) (KOKOTOVIC et al, 1999) and pulsed-field gel electrophoresis (PFGE) (STAKENBORG et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection, quantification and genetic variability of Mycoplasma hyopneumoniae from apparently healthy and pneumonic swine

Pulgarón¹,

Marques

Campos

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

Molecular differences among Mycoplasma hyopneumoniae strains present in pneumonic lungs of swine have been largely studied. However, no comparative studies concerning the strains present in apparently healthy pigs have been carried out. This study aimed to detect, quantify and perform molecular analysis of M. hyopneumoniae strains in pig lungs with and without pneumonic lesions. The detection of M. hyopneumoniae was performed using multiplex PCR (YAMAGUTI, 2008), real-time PCR (STRAIT et al., 2008) and multiple VNTR amplification (VRANCKX et al., 2011). Molecular characterization of the strains was achieved by analysis of the VNTR copy number in P97R1, P146R3, H2R1 and H4. M. hyopneumoniae was detected in samples from healthy and pneumonic pigs and the amount of M. hyopneumoniae positive samples detected varied with the type of assay. The greater number of positive samples was identified by the multiple VNTR amplification combined with capillary electrophoresis. Using real-time PCR, 4.9*10 4 M. hyopneumoniae genome copies/mL was detected in apparently healthy lungs. A mean quantity of 3.9*10 6 M. hyopneumoniae genome copies/mL was detected in pneumonic lungs. The analysis of VNTR copy number demonstrated a high genetic variability of the M. hyopneumoniae strains present in apparently healthy and pneumonic lungs. Strains having 3 VNTR copy number in P97R1, were detected only in pneumonic lungs and strains having 40 and 43 VNTR copy number in P146R3 were detected only in apparently healthy lungs. Despite the genetic variability of M. hyopneumoniae, predominant strains in the swine farms could be identified.Keywords: M. hyopneumoniae. Enzootic pneumonia. Healthy. Quantification. VNTR. ResumoAs diferenças moleculares entre as estirpes de Mycoplasma hyopneumoniae presentes em pulmões de suínos com pneumonia têm sido estudadas. Porém, estudos comparativos relativos às estirpes presentes nos suínos aparentemente saudáveis não foram levados a cabo. O objetivo do estudo foi a detecção, quantificação e análise molecular de M. hyopneumoniae nos pulmões suínos com e sem lesões pneumônicas. Para a detecção de M. hyopneumoniae usaramse o PCR Múltiplo (YAMAGUTI, 2008), o PCR a Tempo Real (STRAIT et al., 2008) e a amplificação de múltiplo VNTR (VRANCKX et al., 2011). A caracterização molecular das estirpes foi realizada mediante a análise do número de cópias de VNTR em P97R1, P146R3, H2R1 e H4. O M. hyopneumoniae foi detectado em amostras de suínos saudáveis e pneumônicos e a quantidade de M. hyopneumoniae nas amostras positivas variou com o tipo de ensaio. O maior número de amostras positivas foi identificado pela amplificação de múltiplas VNTR combinado com a eletroforese de capilares. Usando o PCR a Tempo Real, 4.9*10 4 cópias de genoma/mL de M. hyopneumoniae foram detectadas em pulmões aparentemente saudáveis. Uma quantidade média de 3.9*10 6 cópias de genoma/mL de M. hyopneumoniae foi detectada em pulmões pneumônicos. A análise do número de cópias de VNTR demonstrou uma elevada variabilidade genética das estirpes d...

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Detection, quantification and genetic variability of Mycoplasma hyopneumoniae from apparently healthy and pneumonic swine

Pulgarón¹,

Marques

Campos

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…Therefore, it cannot be excluded that only the dominant strains in the herd were detected [9]. Although it is known from previous studies that pigs may be infected with more than one strain [9, 11, 12], the results of the present study in vaccinated herds document a higher diversity of M. hyopneumoniae strains than shown by previous authors [6, 22–24]. The results also suggest that vaccination of piglets against M. hyopneumoniae does not lead to an important decrease in the diversity of M. hyopneumoniae strains in slaughter pigs.…”

Section: Discussionmentioning

confidence: 99%

Impact of diversity of Mycoplasma hyopneumoniae strains on lung lesions in slaughter pigs

Michiels

Vranckx²,

Piepers

et al. 2017

Vet Res

View full text Add to dashboard Cite

The importance of diversity of Mycoplasma hyopneumoniae (M. hyopneumoniae) strains is not yet fully known. This study investigated the genetic diversity of M. hyopneumoniae strains in ten pig herds, and assessed associations between the presence of different strains of M. hyopneumoniae and lung lesions at slaughter. Within each herd, three batches of slaughter pigs were investigated. At slaughter, from each batch, 20 post mortem bronchoalveolar lavage fluid samples were collected for multiple locus variable-number tandem repeat analysis (MLVA), and lung lesions (Mycoplasma-like lesions, fissures) were examined. Multivariable analyses including potential risk factors for respiratory disease were performed to assess associations between the number of different strains per batch (three categories: one strain, two–six strains, ≥seven strains), and the lung lesions as outcome variables. In total, 135 different M. hyopneumoniae strains were found. The mean (min.–max.) number of different strains per batch were 7 (1–13). Batches with two–six strains or more than six strains had more severe Mycoplasma-like lesions (P = 0.064 and P = 0.012, respectively), a higher prevalence of pneumonia [odds ratio (OR): 1.30, P = 0.33 and OR: 2.08, P = 0.012, respectively], and fissures (OR = 1.35, P = 0.094 and OR = 1.70, P = 0.007, respectively) compared to batches with only one strain. In conclusion, many different M. hyopneumoniae strains were found, and batches of slaughter pigs with different M. hyopneumoniae strains had a higher prevalence and severity of Mycoplasma-like lung lesions at slaughter, implying that reducing the number of different strains may lead to less lung lesions at slaughter and better respiratory health of the pigs.

show abstract

“…Finally, the model does neither account for multiple infections with several strain of M. hyopneumoniae nor for virulence of the one or more strains in particularly infected herds. Infections with more than one strain have been reported [34] and also virulence might vary significantly [1], but lack of detailed information about impact on parameters and a tremendous increase of computing power needed to account for these factors in the model precluded further consideration. It is assumed that in herds infected with a low virulent strain, all effects will be smaller, whereas in a herd infected with a high virulent strain all effects might be more sever.…”

Section: Discussionmentioning

confidence: 99%

Modelling the within-herd transmission of Mycoplasma hyopneumoniae in closed pig herds

Fournié

Wieland²,

Pfeiffer

et al. 2016

Porc Health Manag

Self Cite

View full text Add to dashboard Cite

BackgroundA discrete time, stochastic, compartmental model simulating the spread of Mycoplasma hyopneumoniae within a batch of industrially raised pigs was developed to understand infection dynamics and to assess the impact of a range of husbandry practices. A ‘disease severity’ index was calculated based on the ratio between the cumulative numbers of acutely and chronically diseased and infectious pigs per day in each age category, divided by the length of time that pigs spent in this age category. This is equal to the number of pigs per day, either acutely or chronically infectious and diseased, divided by the number of all pigs per all days in the model. The impact of risk and protective factors at batch level was examined by adjusting ‘acclimatisation of gilts’, ‘length of suckling period’, ‘vaccination of suckling pigs against M. hyopneumoniae’, ‘contact between fattening pigs of different age during restocking of compartments’ and ‘co-infections in fattening pigs’.ResultsThe highest ‘disease severity’ was predicted, when gilts do not have contact with live animals during their acclimatisation, suckling period is 28 days, no vaccine is applied, fatteners have contact with pigs of other ages and are suffering from co-infections. Pigs in this scenario become diseased/infectious for 26.1 % of their lifetime. Logistic regression showed that vaccination of suckling pigs was influential for ‘disease severity’ in growers and finishers, but not in suckling and nursery pigs. Lack of contact between gilts and other live pigs during the acclimatisation significantly influenced the ‘disease severity’ in suckling pigs but had less impact in growing and finishing pigs. The length of the suckling period equally affected the severity of the disease in all age groups with the strongest association in nursery pigs. The contact between fatteners of different groups influenced the course of infection among finishers, but not among other pigs. Finally, presence of co-infections was relevant in growers and finishers, but not in younger pigs.ConclusionThe developed model allows comparison of different prevention programmes and strategies for controlling transmission of M. hyopneumoniae.Electronic supplementary materialThe online version of this article (doi:10.1186/s40813-016-0026-1) contains supplementary material, which is available to authorized users.

show abstract

RAPD and VNTR analyses demonstrate genotypic heterogeneity of Mycoplasma hyopneumoniae isolates from pigs housed in a region with high pig density

Cited by 31 publications

References 31 publications

Detection, quantification and genetic variability of Mycoplasma hyopneumoniae from apparently healthy and pneumonic swine

Detection, quantification and genetic variability of Mycoplasma hyopneumoniae from apparently healthy and pneumonic swine

Impact of diversity of Mycoplasma hyopneumoniae strains on lung lesions in slaughter pigs

Modelling the within-herd transmission of Mycoplasma hyopneumoniae in closed pig herds

Contact Info

Product

Resources

About