RAPD and virulence analyses of Colletotrichum capski isolates from chilli (Capsicum annuum)

Madhavan, S.; Paranidharan, V.; Velazhahan, R.

doi:10.1007/bf03356370

Cited by 13 publications

(7 citation statements)

References 7 publications

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“…Toth et al (2004) also reported that no correlation could be observed between clustering of the isolates of Fusarium culmorum based on RAPD, restriction analysis of the intergenic spacer region of sequence data and mycotoxin-producing abilities or aggressiveness. Madhavan et al (2010) also reported no relationship between clustering in a dendrogram based on RAPD data and virulence of C. capsici isolates from chillies. Regular epiphytotics of red rot have been attributed to the appearance of new virulent isolates of pathogen.…”

Section: Resultsmentioning

confidence: 93%

“…Pathogen diversity plays a major role in disease dynamics and consequently, in the success of disease management strategies, including the development of cultivars resistant to diseases. Random Amplified Polymorphic DNA (RAPD) analysis has been used successfully to assess diversity among isolates of other Colletotrichum species (Kelemu et al 1999;Denoyes-Rothan et al 2003;Padder et al 2007;Madhavan et al 2010). In the present study, different isolates of C. falcatum from Tamil Nadu, India were analysed for their ability to produce toxin and genetic diversity using RAPD analysis.…”

Section: Introductionmentioning

confidence: 99%

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Variations in random amplified polymorphic DNA profile and toxin production among isolates ofColletotrichum falcatumWent from sugarcane in Tamil Nadu, India

Nithya

Bukhari

Valluvaparidasan

et al. 2012

Archives Of Phytopathology And Plant Protection

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Red rot, caused by Colletotrichum falcatum Went, is one of the most important diseases of sugarcane (Saccharum officinarum L.). The pathogen shows a great diversity in virulence as a number of pathotypes are known to occur in nature. In the present study, the toxin producing ability and genetic variability among isolates of C. falcatum collected from major sugarcane growing areas of Tamil Nadu, India were analysed. The C. falcatum isolates differed significantly in their ability to produce toxin in vitro. The toxin from C. falcatum isolate Cf 671a induced the maximum electrolyte leakage (300 mS) from sugarcane leaf tissues. The genetic relatedness of the isolates of C. falcatum differing in toxin production potential was investigated by using RAPD analysis. Analysis of the genetic coefficient matrix derived from the scores of RAPD profiles showed that minimum and maximum percent similarities among the tested C. falcatum isolates were in the range of 19 to 95% respectively. The phylogenetic analysis by the UPGMA identified two main clusters. Cluster A contains only one isolate (Cf 98061) and all the other isolates were placed in Cluster B confirming high genetic diversity among the isolates. No correlation was observed between clustering of the C. falcatum isolates in the dendrogram and their toxin producing abilities.

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Section: Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Variations in random amplified polymorphic DNA profile and toxin production among isolates ofColletotrichum falcatumWent from sugarcane in Tamil Nadu, India

Nithya

Bukhari

Valluvaparidasan

et al. 2012

Archives Of Phytopathology And Plant Protection

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“…The fungus, Colletotrichum capsici was isolated from chilli fruits showing symptoms of anthracnose disease and maintained on potato dextrose agar (PDA) medium under laboratory conditions (Madhavan et al, 2010). Burkholderia sp.…”

Section: Materials and Methods Fungal And Bacterial Culturesmentioning

confidence: 99%

Foliar application of Burkholderia sp. strain TNAU-1 leads to activation of defense responses in chilli (Capsicum annuum L.)

Madhavan

Paranidharan

Velazhahan

2011

Braz. J. Plant Physiol.

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Induction of phenolics, peroxidase (PO), polyphenol oxidase (PPO), chitinase and thaumatin-like proteins (TLP) in leaves of chilli (Capsicum annuum L.) in response to foliar application with a biocontrol agent Burkholderia sp. strain TNAU-1 was studied. Chilli plants, when sprayed with Burkholderia sp. strain TNAU-1 showed increase in phenolic content one day after application and the maximum accumulation was observed seven days after treatment. Three new peroxidase isozymes (PO-1, PO-2 and PO-3) were induced in chilli leaves upon treatment with Burkholderia sp. strain TNAU-1. The activity of all the peroxidases was at the maximum level three days after treatment and subsequently decreased. Protein extracts obtained from Burkholderia sp. strain TNAU-1 treated plants exhibited a major polyphenol oxidase (PPO-1) one day after treatment. The activity of PPO-1 subsequently decreased, but continuously present throughout the experimental period of 15 days. Western blot analysis of protein extracts from Burkholderia sp. strain TNAU-1 treated chilli leaves revealed that two TLPs with sizes of 23 and 25 kDa and a chitinase with an apparent molecular weight of 28 kDa were induced three days after treatment

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“…, ; Madhavan et al . ). This study reports the variability of Colletotrichum isolates correlated with the chilli anthracnose in north‐eastern India using RAPD and ISSR markers.…”

Section: Discussionmentioning

confidence: 97%

Molecular, phenotypic and pathogenic variability inColletotrichumisolates of subtropical region in north-eastern India, causing fruit rot of chillies

2014

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RAPD and virulence analyses of Colletotrichum capski isolates from chilli (Capsicum annuum)

Cited by 13 publications

References 7 publications

Variations in random amplified polymorphic DNA profile and toxin production among isolates ofColletotrichum falcatumWent from sugarcane in Tamil Nadu, India

Variations in random amplified polymorphic DNA profile and toxin production among isolates ofColletotrichum falcatumWent from sugarcane in Tamil Nadu, India

Foliar application of Burkholderia sp. strain TNAU-1 leads to activation of defense responses in chilli (Capsicum annuum L.)

Molecular, phenotypic and pathogenic variability inColletotrichumisolates of subtropical region in north-eastern India, causing fruit rot of chillies

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