RAPD and RFLP markers tightly linked to the locus controlling carnation (Dianthus caryophyllus) flower type

Scovel, G.; Ben-Meir, Hagit; Ovadis, Marianna; Itzhaki, Hanan; Vainstein, Alexander

doi:10.1007/s001220050717

Cited by 54 publications

(25 citation statements)

References 17 publications

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“…Double flowers are often preferred by consumers because they are larger, more floriferous, and more showy than single flowers. A double flower is a dominant trait in carnations (Scovel et al, 1998;Yagi et al, 2014), petunias (Liu et al, 2016), and roses (Crespel et al, 2002). In Matthiola incana (stock), a double flower is a recessive trait, and the allele for the double-flower phenotype is closely linked to a lethal allele (Ecker et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Development of DNA Markers Linked to Double-Flower and Hortensia Traits in <i>Hydrangea macrophylla</i> (Thunb.) Ser.

et al. 2018

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Double flower and hortensia (mophead) hydrangea (Hydrangea macrophylla (Thunb.) Ser.) traits are recessively inherited. Cross breeding of these traits in hydrangea is difficult because it takes about two years from crossing to flowering. In this study, we aimed to obtain DNA linkage markers that would allow accelerated selection of these traits. We used next-generation sequencing to comprehensively collect DNA sequences from the 'Kirakiraboshi' with a double flower and lacecap inflorescence and the 'Frau Yoshimi' with a single flower and hortensia inflorescence, and designed simple sequence repeat (SSR) primer pairs for map construction. We screened 768 SSR primer pairs in 93 F 2 progeny derived from 'Kirakiraboshi' and 'Frau Yoshimi'. We identified 147 loci, which were expanded to 18 linkage groups with a total map length of 980 cM. Linkage analysis identified that both the double flower trait from 'Kirakiraboshi' (d Kira ) and the hortensia trait from 'Frau Yoshimi' (h Frau ) were located on linkage group KF_4. Detailed linkage analysis using 351 F 2 progeny revealed a 34.8 cM map length between the two loci and identified two tightly linked SSR markers, STAB045 for d Kira and HS071 for h Frau . Genetic analysis suggested that double flower and hortensia traits are each controlled by a single recessive gene. Together, the linkage map, SSR markers, and genetic information obtained in this study will be useful for future hydrangea breeding.

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Section: Discussionmentioning

confidence: 99%

Development of DNA Markers Linked to Double-Flower and Hortensia Traits in <i>Hydrangea macrophylla</i> (Thunb.) Ser.

et al. 2018

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“…For example, some standard-and spray-type varieties do not have easily distinguishable flower characteristics due to phenotypic overlaps caused by environmental conditions, even if standard-and spray-type varieties usually have double and semi-double flower characteristics, respectively (Scovel et al, 1998).…”

Section: Genetic Variation Evaluated By Ssr Markersmentioning

confidence: 99%

“…Recently, molecular markers have been developed and used to identify several characteristics controlling flower longevity (De Beneditti et al, 2003, 2005, resistance to disease (Onozaki et al, 1999(Onozaki et al, , 2004Scovel et al, 2001;Yagi et al, 2006), and double flowers (Scovel et al, 1998) in carnation breeding programs. Molecular markers have been also utilized to confirm interspecific hybrids (Nimura et al, 2003(Nimura et al, , 2006 and variety identification (Smulders et al, 2000(Smulders et al, , 2003.…”

Section: Introductionmentioning

confidence: 99%

Development of SSR Markers in Carnation (Dianthus caryophyllus)

Kimura

Yagi

Nishitani

et al. 2009

J. Japan. Soc. Hort. Sci.

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Thirteen SSR (simple sequence repeat) markers were successfully developed in carnation using an enriched genomic library method. All 13 primer pairs designed could successfully amplify target fragments of the expected size for the original variety 'Francesco' or 'Barbara'. Thirty-nine of 41 carnation varieties, except for mutant varieties, were successfully distinguishable using 32 putative alleles and 15 polymorphic bands produced from 13 SSR markers. 'U Conn Sim' and 'White Sim', which are bud mutants originated from 'William Sim', showed an identical genotype to 'William Sim'. Three (CB026a, CB057a, and CB060a) of 13 SSR markers were thought to be derived from multi-locus because the 3 SSR markers produced more than 3 discrete amplified fragments. The number of alleles per locus ranged from 1 (CB020a and CB041a) to 9 (CB003a), with an average value of 3.2. The values of the observed heterozygosity (H O ) and the expected heterozygosity (H E ) ranged from 0 to 0.59 (mean 0.30) and 0 to 0.68 (mean 0.34), respectively. The power of discrimination (PD) ranged from 0 (CB020a and CB041a) to 0.93 (CB026a) (mean 0.49). The parentage of 2 carnation varieties from intraspecific crosses was analyzed using 10 SSR markers, and the hybridity of the progenic varieties was confirmed. SSR markers were very effective for variety discrimination and determination of carnation parentage.

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“…Figueira et al (1992) reported that the nuclear DNA content of carnation was 1.23 pg/2C, which indicates that the carnation has a comparatively small nuclear genome of about 4-fold size of Arabidopsis thaliana (0.30 pg/2C) (Arumuganathan and Earle, 1991). In carnation, a few molecular markers linked to traits, such as flower type (Scovel et al, 1998), resistance to Fusarium oxysporum f. sp. dianthi (Scovel et al, 2001), and post-harvest longevity of cut flowers (De Benedetti et al, 2003) have been reported previously by using random amplified polymorphic DNA (RAPD) analysis.…”

Section: Introductionmentioning

confidence: 99%

Construction of a Genetic Linkage Map for the Carnation by Using RAPD and SSR Markers and Mapping Quantitative Trait Loci (QTL) for Resistance to Bacterial Wilt Caused by Burkholderia caryophylli

雅史¹,

隆²,

光泰³

et al. 2006

J. Japan. Soc. Hort. Sci.

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5Tochigi Prefectural Minaminasu Agriculture Promotion Office, Karasuyama, Tochigi, 321-0621, Japan We constructed a genetic linkage map for the carnation (Dianthus caryophyllus L.) on the basis of random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) by using a resistance-segregating population of 134 progeny lines that were derived from a cross between 'Carnation Nou No. 1' (a carnation breeding line resistant to bacterial wilt) and 'Pretty Favvare' (a susceptible cultivar). The linkage map consisted of 137 RAPD and 9 SSR markers. Linkage analysis revealed that 124 loci could be mapped to 16 linkage groups that extended for 605.0 centiMorgans (cM). The average interval between two loci was 4.9 cM. Quantitative trait loci (QTL) analysis was applied to evaluations of resistance to bacterial wilt that were replicated 8 times. The QTL that we reported previously with a large effect on resistance was detected on group 6 which accounted for 60.5% of the total phenotypic variance with a LOD score 23.46. Two other QTLs with small effects were detected on groups 2 and 5 with LOD scores of 2.32 and 2.87, respectively. These results suggest that resistance to bacterial wilt in carnation is related to one major and at least two minor genes. This study is the first report on the construction of a linkage map of the carnation.

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RAPD and RFLP markers tightly linked to the locus controlling carnation (Dianthus caryophyllus) flower type

Cited by 54 publications

References 17 publications

Development of DNA Markers Linked to Double-Flower and Hortensia Traits in <i>Hydrangea macrophylla</i> (Thunb.) Ser.

Development of DNA Markers Linked to Double-Flower and Hortensia Traits in <i>Hydrangea macrophylla</i> (Thunb.) Ser.

Development of SSR Markers in Carnation (Dianthus caryophyllus)

Construction of a Genetic Linkage Map for the Carnation by Using RAPD and SSR Markers and Mapping Quantitative Trait Loci (QTL) for Resistance to Bacterial Wilt Caused by Burkholderia caryophylli

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