RAPD and Protein Analyses Revealed Polymorphism in Mutated Potato Cultivars

Abstract

Genomic DNA of the mutant lines of the three potato cultivars, Cardinal, Diamant and Desiree, with respect to controls were isolated and analyzed for polymorphisms by using random amplified polymorphic DNA (RAPD) markers. Four 10 bp random fragment primers, S-13, S-18, S-19 and R-17 were studied and all of them gave the amplification of genomic DNA. All of the mutant lines gave different banding pattern against different primers with respect to control plants of the three varieties, and bands are present at 50 bp to 1500 bp. All these primers with specific banding pattern were unique in their polymorphic behavior. Different banding pattern of total protein contents were also observed by PAGE analysis of all the mutant lines as compared with the control plants. It is therefore suggested that RAPD and protein analyses would be important tools to detect the polymorphism in mutated lines of potato.

Molecular characterization and diversity analysis of some local potato (Solanum tuberosum L.) genotypes of Bangladesh by RAPD markers

“…AL-Salihy et al (2014) screened 6 RAPD primers on four potato genotypes and successfully studied genetic diversity with five primers. 'Band size range' proximate to this analysis was found byPattanayak et al (2004) andAhmad et al (2013). Moreover, Verma and Singh (2019) reported 1.9 bands per potato cultivar and 4.55 bands per primer, which were lower than the present study regarding bands per genotypes and bands per primer.…”

Molecular Diversity Analysis of Somaclonal Variants of Potato (Solanum tuberosum L.) by Random Amplified Polymorphic DNA Markers

“…This variation may be due to the difference in genotypes and primers used. Ahmad et al [28] studied 4 RAPD primers in mutant lines of Cardinal, Diamant and Desiree and found bands were present between 50 bp to 1500 bp. Which is less than our overall band size range but close to majority band size range.…”